Molecular community analysis of microbial diversity

Gel Electrophoresis Based Genetic Fingerprinting Techniques on Environmental Ecology

“…Therefore 16S rRNA and its encoding gene have been widely used to investigate community diversity. The rapidly growing 16S rDNA sequence data bank, accessible (http://www.ebi.ac.uk/) provides the opportunity to get information about 16S rDNA sequences of the determined cultured and uncultured species (Dahllöf, 2002).…”

“…In spite of the advantages of using 16S rRNA molecule for phylogenetic analysis, the main limitations are that the heterogeneity between multiple copies of this molecule in one species interferes pattern analysis, confuses the explanation of diversity obtained from clone libraries and sequences retrieved from banding patterns (Dahllöf, 2002).…”

Peer Reviewed: Genomics Tools in Environmental Impact Assessment

“…Microbial communities are key driving forces through which essential ecological services are rendered to higher microorganisms of that ecosystem (9,17,18). The ecological services are part of the biogeochemical cycle, which is catalyzed by the biochemical potential of microbial communities (12).…”

Phylum- and Class-Specific PCR Primers for General Microbial Community Analysis

“…This implies that assays using these primers will detect any major shift in community structure. In practice, however, sampling issues make rare sequences difficult to detect, and there can be PCR bias toward organisms that are dominant or have preferentially amplified sequences (8,27). In genetic profiling methods, the ability to distinguish between taxa is lowered by broad primers because phylogenetically disparate organisms can have similar genetic markers (5,12).…”