Molecular comparative assessment of the microbial ecosystem in rumen and faeces of goats fed alfalfa hay alone or combined with oats

Mohammadzadeh, Hamid; Yáñez-Ruíz, D. R.; Fernández, Gonzalo Martínez; Abecia, Leticia

doi:10.1016/j.anaerobe.2013.11.012

Cited by 20 publications

(18 citation statements)

References 30 publications

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“…At genus level, Prevotella was shared by all samples and had the highest abundance both in CF and AF in our study (Table 4), which was in accordance with previous studies in other ruminants [3,9]. Analyzing the previous experiments, we found that the abundance of Prevotella did not always demonstrate regulated changes with the alteration of ration.…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, many kinds of ruminants including cows, cattle, buffalo, yak and sheep have been used as experimental subjects to determine the microbial diversity in rumen [2,6,7], while goats are rarely used in studies. And, the previous goat-related studies [8,9] mainly used conventional methods, such as molecular fingerprint, to evaluate the ruminal microbial community. These methods could only identify the dominant species instead of the complete composition of the microorganisms in sample when microbial diversity was investigated [10].…”

Section: Introductionmentioning

confidence: 99%

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The impact of diet on the composition and relative abundance of rumen microbes in goat

Wang

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveThis experiment was conducted to explore the impact of diet on the ruminal microbial community in goats.MethodsTwelve goats were divided into two groups and fed complete feed (CF) or all forage (AF) diet. The total microbial DNAs in the rumen liquid were extracted. The V4 region of microbial 16S rRNA genes was amplified and sequenced using high-throughput. Information of sequences was mainly analyzed by QIIME 1.8.0.ResultsThe results showed that Bacteroidetes and Firmicutes were the most predominant microbial phyla in the rumen of all goats. At genus level, the abundance of fiber-digesting bacteria such as Ruminococcus and Lachnospiracea incertae sedis was significantly higher in AF than that in CF, while the levels of fat-degrading bacterium Anaerovibrio and protein-degrading bacterium Pseudomonas were opposite. The core shared genera, Prevotella and Butyrivibrio were widespread in the rumen of goats and no significant difference was observed in relative abundance between groups.ConclusionWe concluded that the richness of fiber-, protein-, and fat-digesting bacteria was affected by diet and tended to increase with the rise of their corresponding substrate contents in the ration; some bacteria shared by all goats maintained stable despite the difference in the ration, and they might be essential in maintaining the normal function of rumen.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The impact of diet on the composition and relative abundance of rumen microbes in goat

Wang

et al. 2016

Asian-Australas J Anim Sci

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“…Although protozoa have not been found in the ruminant hindgut with microscopic techniques (Gressley et al, 2011), protozoal DNA can be detected in caecum, colon (Lin et al, 1997) and faeces of ruminants (Mohammadzadeh et al, 2014). It is thus likely that protozoal DNA found in the hindgut is derived from dead protozoal cells originating from the rumen.…”

Section: Differences In Microbial and Fermentation Characteristics Bementioning

confidence: 97%

Variability in microbial population and fermentation traits at various sites within the forestomach and along the digestive tract as assessed in goats fed either grass or browse

Zeitz

Ineichen

Soliva

et al. 2016

Small Ruminant Research

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“…Zhang et al (2017) revealed that partial replacement whole corn silage with ensiled 6 dried oat hay significantly affects the composition of the ruminal microflora. Mohammadzadeh et al (2014) compared the biomass and community structure of bacteria, protozoa and archaea communities of rumen and faeces of goats fed on alfalfa with or without oat and found that the diet influences the composition of rumen microbes. Wright et al (2004) demonstrated that rumen methanogen diversity of sheep offered oat hay diet was lower than that of sheep grazing in pasture.…”

Section: Introductionmentioning

confidence: 99%

Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai-Tibetan Plateau

et al. 2019

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Diet is the great determinant of bacterial composition in the rumen. However, little is known about the rumen bacterial community of Tibetan sheep living in the special ecological environment of the Qinghai-Tibetan Plateau (QTP) of China. In the present study, we used high-throughput sequencing to investigate the rumen bacterial community of Tibetan sheep associated with two primary diets: alpine pasture diet (a continuation of the sheep’s natural grazing diet) and oat (Avena sativa) hay diet on the QTP. The results showed that bacterial community richness and species diversity of the oat hay diet group were significantly greater than that of the native pasture diet group (p < 0.05). Principal co-ordinate analysis and analysis of similarities revealed that the bacterial community of the oat hay diet group was distinctly different from that of the native pasture diet group (p < 0.05). Bacteroidetes and Firmicutes were the predominant microbial phyla in the rumen. The rumen of oat-hay-fed sheep had higher proportions of Proteobacteria and novel bacteria species than the rumen of native-pasture-fed sheep. Actinobacteria, an uncommon bacterial phylum, occurred only in the oat-hay-fed group. At the genus level, Komagataeibacter, Ruminococcaceae_UCG-014, and Ruminococcaceae_NK4A214 showed significantly higher relative abundance in the oat-hay-fed sheep than in the native-pasture-fed sheep (p < 0.05). This study is the first of the QTP to employ high-throughput sequencing to examine the influence of diet on the rumen microbiome of Tibetan sheep.

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Molecular comparative assessment of the microbial ecosystem in rumen and faeces of goats fed alfalfa hay alone or combined with oats

Cited by 20 publications

References 30 publications

The impact of diet on the composition and relative abundance of rumen microbes in goat

The impact of diet on the composition and relative abundance of rumen microbes in goat

Variability in microbial population and fermentation traits at various sites within the forestomach and along the digestive tract as assessed in goats fed either grass or browse

Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai-Tibetan Plateau

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