Molecular comparison of Gasterophilus intestinalis and Gasterophilus nasalis from two distinct areas of Poland and Italy based on cox1 sequence analysis

Pawlas-Opiela, M.; Wojciech, Łukasz; Sołtysiak, Zenon; Otranto, Domenico; Ugorski, Maciej

doi:10.1016/j.vetpar.2009.12.030

Cited by 11 publications

(6 citation statements)

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“…A 632 bp fragment of the mitochondrial cox 1 gene was amplified with primers Gco1s (5'-CAG TTG GAA TAG ACG TTG ATA CTC-3') and Gco1an (5'-AGG AAG TTC AGA ATA GCA GTG TTC-3') [ 20 ], and a 688 bp fragment of the mitochondrial cox 2 gene was amplified with primers TL2-J-3037 (5'-ATG GCA GAT TAG TGC AAT GG-3') and TK-N-3785 (5'-GTT TAA GAG ACC AGT ACT TG-3') [ 28 ]. PCR amplification of cox 1 was performed according to a previously described protocol [ 20 ]. PCR conditions for cox 2 were as follows: 94 °C for 2 min, 35 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min, and 25 °C for 2 min.…”

Section: Methodsmentioning

confidence: 99%

“…More specifically, mitochondrial cytochrome c oxidase cox 1 and cox 2 genes have been used to examine inter- and intraspecific relationships in Diptera, Lepidoptera, Coleoptera, Hymenoptera and Hemiptera [ 15 – 19 ]. Previous investigations based on partial mitochondrial cox 1 gene sequences revealed a high degree of genetic diversity that enabled differentiation of the Italian and Polish populations by PCR-restriction fragment length polymorphism [ 20 ]. In G. intestinalis from Daqing (DQ) and Yili (YL), China, genetic information is available for three mitochondrial genes, including cox 1, NADH dehydrogenase subunit 5 gene ( nad 5) and 23S ribosomal RNA gene [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of common Gasterophilus spp. from distinct habitats in China

et al. 2018

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BackgroundGasterophilus species are widely distributed around the world. The larvae of these flies parasitize the digestive tract of equids and cause damage, hindering horse breeding and protection of endangered species. However, study of the genetic structure of geographically distinct Gasterophilus populations is lacking. Here, we analyzed the genetic diversity of Gasterophilus pecorum, G. intestinalis, G. nasalis and G. nigricornis from three typical grasslands (meadow, desert and alpine steppes) in China as compared to published sequences from Italy, Poland and China (Daqing and Yili), based on the mitochondrial cytochrome c oxidase cox1 and cox2 gene sequences.ResultsHaplotype diversity and nucleotide diversity of mitochondrial genes was generally high in all Gasterophilus populations. Due to the unique natural climatic conditions of the alpine steppe, there were high levels of genetic differentiation among different geographical populations of G. pecorum and G. nasalis, indicating that environmental variations influenced population genetic structure. Frequent exchanges between meadow and desert steppe Gasterophilus species resulted in low genetic differentiation. The highest exchange rates were found among G. intestinalis populations. Genetic differentiation was only observed on a large geographical scale, which was confirmed by analyzing population genetic structure. Three species, G. pecorum, G. intestinalis and G. nasalis, from meadow steppe showed a high emigration rate, indicating that the direction of Gasterophilus dispersal in China was from east to west.ConclusionsOur results show that the four Gasterophilus species have a high level of genetic diversity and different degrees of genetic differentiation and gene flow among different populations of the same species, reflecting their potential to adapt to the environment and the environmental impact on genetic structure. Knowledge of the genetic structure, population history, and migration will help understand the occurrence and prevalence of gasterophilosis and provide a basis for controlling the local spread of Gasterophilus spp.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3042-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of common Gasterophilus spp. from distinct habitats in China

et al. 2018

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“…Molecular methods accurately capture the differences among species (Bezeng et al, 2017) and complement morphological data in species identification. Molecular sequence data involving mtDNA COI gene has stronger patterns of genetic variability than those of nuclear regions (Li et al, 2014a), and the COI barcode region has been a verified effective and informative tool employed in animal identification (Hebert et al, 2010;Pawlas-Opiela et al, 2010;Li et al, 2014b) including ticks (Lv et al, 2014;Zhang and Zhang, 2014;Csordas et al, 2016;Ghosh et al, 2020;Davari et al, 2021). Integrative morphological and molecular identification of ticks gives a clear and more reliable result compared to single identification method (Ghosh et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Dna Barcoding of Ixodid Ticks Infesting Cattle and Sheep in Nsukka, Nigeria

Onah

Ngwu²,

Ngabuozor³

et al. 2023

FJS

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Ticks constitute one of the major health challenges to livestock production in Nigeria. They are particularly important due to their blood-feeding activity causing anaemia and as vectors of pathogens causing many livestock diseases. Accurate identification of ticks infesting livestock is fundamental for effective control of tick and tick-borne diseases. We integrated morphological identification and DNA barcoding to unambiguously identify the species of ticks and assessed their prevalence on cattle and sheep in Nsukka, Nigeria. All the animals in the farm: 37 cattle and 22 sheep were examined. All the cattle examined (100%) and 9 (40.09%) of the sheep were infested with ticks. The ticks Amblyomma variegatum and Rhipicephalus microplus were found on cattle and sheep in Nsukka. A total of 317 ticks were collected out of which 272 were on cattle and 45 on sheep. Of the total number of ticks collected, 152 (47.95%) were A. variegatum while 165 (52.05%) were R. microplus. Tick infestation was significantly higher on cattle than sheep (p<0.05, χ 2 = 3.902, p = 0.0482, n=59). The barcode region of COI gene was successfully amplified and sequenced in all the samples of ticks analysed. Samples of A. variegatum were identified at 99.70-100%. Similarly, samples of R. microplus were identified at 100%. Phylogenetic analysis clustered A. variegatum and R. microplus with their respective species from GenBank on phylogenetic tree. The ticks exhibited morphological ambiguity and sexual dimorphism, but DNA barcoding resolved the identities of the ticks. Integrative taxonomy is the best for tick identification.

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“…Due to the significant veterinary and economic importance of Gasterophilus species, they have received considerable attention regarding identification, distribution, infectious intensity and incidence [15–18]. It has been suggested that species of Gasterophilus may be threatened with extinction because of increased and widespread use of broad spectrum antiparasitics (e.g.…”

Section: Introductionmentioning

confidence: 99%

Gasterophilus flavipes (Oestridae: Gasterophilinae): A horse stomach bot fly brought back from oblivion with morphological and molecular evidence

Pape

Zhang

2019

PLoS ONE

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Species of Gasterophilus Leach are obligate parasites in domestic and wild equids and responsible for cosmopolitan gasterophilosis. Although with only eight species known so far, they have received considerable attention because of their significant veterinary and economic importance. Surprisingly, we found that G . flavipes (Olivier) is a valid species based on morphological characters from male, female and the egg, after spending half a century as a synonym of G . haemorrhoidalis (Linnaeus). In the present study, G . flavipes , G . haemorrhoidalis and G . inermis (Brauer), which are the three closely related species possessing a remarkable mixture of shared morphological characters, are diagnosed and comparatively redescribed; the key to separate adults and eggs are provided, together with a series of high-resolution photographs from all the body parts. COI barcodes do not allow for a separation of G . flavipes , G . haemorrhoidalis and G . inermis , but showed a closer relationship between G . flavipes and G . haemorrhoidalis than the other two combinations, which is consistent with the morphological evidence. Geographically, G . flavipes seems to be common and widespread in the warmer parts of the Palaearctic region. Thus, the epidemiology of gasterophilosis where G . flavipes is known or supposed to occur calls for a more careful veterinarian re-assessment. A decline in the populations of Gasterophilus spp. has been noticed in Europe, but all seven Palaearctic species of Gasterophilus appear to maintain stable populations in Xinjiang (China), which may be explained by a higher biodiversity of equids and less use of anti-parasitic treatments in Xinjiang than in Europe. Our study shows that morphological characters still provide the solid backbone in classification of Gasterophilus at species-level, and updated diagnoses and a key is provided to distinguish G . flavipes , G . haemorrhoidalis and G . inermis , and to facilitate studies of epidemiology, phylogeny and host-parasite co-evolution.

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Molecular comparison of Gasterophilus intestinalis and Gasterophilus nasalis from two distinct areas of Poland and Italy based on cox1 sequence analysis

Cited by 11 publications

References 9 publications

Genetic diversity of common Gasterophilus spp. from distinct habitats in China

Genetic diversity of common Gasterophilus spp. from distinct habitats in China

Dna Barcoding of Ixodid Ticks Infesting Cattle and Sheep in Nsukka, Nigeria

Gasterophilus flavipes (Oestridae: Gasterophilinae): A horse stomach bot fly brought back from oblivion with morphological and molecular evidence

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