Molecular Confirmation of a Fasciola Gigantica × Fasciola Hepatica Hybrid in a Chadian Bovine

Evack, Jennifer Giovanoli; Schmidt, Roman; Boltryk, Sylwia D.; Voss, Till S.; Batil, Annour Adoum; Ngandolo, Bongo Naré Richard; Greter, Helena; Utzinger, Jürg; Zinsstag, Jakob; Balmer, Oliver

doi:10.1645/19-66

Cited by 20 publications

(12 citation statements)

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“…In areas where both species coexist, F. hepatica x F. gigantica hybrids have been found [31][32][33]. Recently, the presence of hybrids has also been confirmed in sub-Saharan Africa [34]. Fascioliasis is endemic in West Africa with the predominant species being F. gigantica [35,36].…”

Section: Introductionmentioning

confidence: 98%

Prevalence and distribution of livestock schistosomiasis and fascioliasis in Côte d’Ivoire: results from a cross-sectional survey

Kouadio

Evack

Achi³

et al. 2020

BMC Vet Res

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Background Schistosoma and Fasciola are zoonotic parasites of public health and veterinary importance. However, while the epidemiology of schistosomiasis in humans is well studied, little is known about fascioliasis and schistosomiasis in livestock in Côte d’Ivoire. This study aimed to determine the prevalence and the distribution of livestock schistosomiasis and fascioliasis across Côte d’Ivoire. In 2018, we conducted a cross-sectional survey in abattoirs and farms in 13 departments of Côte d’Ivoire. In abattoirs, the mesenteric veins and livers of slaughtered cattle, sheep and goats were examined for adult Schistosoma and Fasciola flukes. Faeces from live cattle, goats and sheep were collected and examined for Schistosoma and Fasciola eggs using a sedimentation technique. Results A total of 386 cattle, 174 goats and 151 sheep from abattoirs and 435 cattle, 22 goats and 176 sheep from farms were sampled. The observed prevalence of schistosomiasis was higher in slaughtered animals. Fascioliasis was more prevalent in farm animals. The prevalence of schistosomiasis in slaughtered cattle varied between 5.9% (95% confidence interval (CI): 0.7–19.7%) and 53.3% (95% CI: 37.9–68.3%) with the highest prevalence observed in Ouangolodougou in the North. Cattle from farms had a relatively low prevalence of schistosomiasis, with the highest prevalence found in Ouangolodougou (2.4%, 95% CI: 0.7–6.1%). The prevalence of fascioliasis varied considerably from one department to another, ranging from nil (95% CI: 0.0–18.5%) to 50.8% (95% CI: 43.4–58.2%), with the highest prevalence found in farm cattle in Dikodougou in the North. Sheep and goats had a lower prevalence of schistosomiasis and fascioliasis than cattle. In slaughtered animals, cattle aged 4 years and older were at highest risk for schistosomiasis (odds ratio (OR): 2.4; 95% CI: 1.0–5.6) and fascioliasis (OR: 2.1; 95% CI: 1.1–3.9). In farm animals, male cattle had higher odds of being infected with Schistosoma (OR: 4.3; 95% CI: 0.7–26.9) than females. Conclusions Our study confirms that schistosomiasis and fascioliasis are endemic in livestock across Côte d’Ivoire. A strategic control programme should be considered, especially for cattle, including providing drinking water in troughs to reduce faecal contamination of water sources by cattle.

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Section: Introductionmentioning

confidence: 98%

Prevalence and distribution of livestock schistosomiasis and fascioliasis in Côte d’Ivoire: results from a cross-sectional survey

Kouadio

Evack

Achi³

et al. 2020

BMC Vet Res

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“…However, the traditional morphological methods used to distinguish the two species may be unreliable, especially in the presence of hybrids that result in intermediate forms in endemic areas where the two species overlap [14]. Previous reports have shown that genetic hybridization can be detected in Fasciola species using the ribosomal internal transcribed spacer (ITS) region and mitochondrial DNA as genetic markers [4,14]. In Vietnam, a report, which analyzed 14 samples extracted from humans, presented the majority of F. gigantica (9/14).…”

Section: Discussionmentioning

confidence: 99%

“…The development of molecular methods enables specieslevel identification and provides a powerful tool for identifying potential hybrid species [3]. Currently, there have been several reports of a hybrid of F. hepatica and F. gigantica in animals worldwide [4]. However, this liver fluke has rarely been detected in humans.…”

Section: Introductionmentioning

confidence: 99%

Biliary fascioliasis – A rare differential diagnosis of biliary obstruction

Ngo

Phuoc

et al. 2022

J Infect Dev Ctries

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Fascioliasis is a zoonotic disease caused by Fasciola hepatica or F. gigantica. There are two phases of Fasciola infection. In the acute phase, the patient has a wide spectrum of clinical manifestations while the later phase of infection is usually related to inflammatory changes in the bile duct and the mechanical effects of the worm, which can lead to biliary obstruction. The presence of Fasciola inside the biliary system has rarely been reported in the literature. In this report, we presented a case of bile duct obstruction in a 36-year-old male patient undergoing cholecystectomy for gallstones and with a history of worsening jaundice for one month. Extensive laboratory testing failed to clarify the cause of jaundice. Three living liver flukes were subsequently retrieved from the right hepatic duct and later confirmed as F. gigantica by morphology and internal transcribed spacer (ITS2) sequence analysis.

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“…A study in Niger also showed that 66.7% of 12 cattle isolates were F. gigantica (Ali et al 2008). In the past, it was believed that F. hepatica was present primarily in the temperate regions, while F. gigantica is distributed in some countries in tropical region (Evack et al 2020). This has been proved not to be the case because several , including this present study that have shown the existence of both F. hepatica and F. gigantica in the three localities in Kenya.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

Kipyegen

Muleke

Otachi

2021

Preprint

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Fasciolosis is a neglected trematode infection of public health and veterinary importance caused by Fasciola gigantica and Fasciola hepatica. Molecular analysis using the internal transcribed spacers’ ITS-1 and ITS-2 of nuclear ribosomal DNA is useful in distinguishing Fasciola species. This study aimed to characterize liver flukes from sheep, goats and cattle using these genetic markers. Fifty nine adult Fasciola specimens were collected from livers of naturally infected sheep, goats and cattle at selected abattoirs in Kisumu, Baringo and Narok Counties. Sequence comparison of ITS-1 and ITS-2 sequences of Fasciola isolates from this study and sequences in Genbank was carried out. A maximum likelihood tree was constructed for phylogenetic analysis. Analysis of ITS-1 and ITS-2 rDNA sequences revealed that F. hepatica and F. gigantica caused infection in both cattle and sheep and in goats only F. gigantica caused infection. The sequenced PCR amplicons showed a close relationship between Fasciola species in this study with Fasciola isolates from other regions in the world. Phylogenetic analysis showed that sequences of F. hepatica are similar to the sequence from Spain, China and Tunisia obtained from GenBank. The sequences of F. gigantica in this study have similarity to the sequence from Iran and Burkina Faso. Data from this study provides information that serves as basis for further studies on the distribution of F. gigantica and F. hepatica in other localities in Kenya, and is also important in designing epidemiological and control programmes for zoonotic fascioliasis.

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Molecular Confirmation of a Fasciola Gigantica × Fasciola Hepatica Hybrid in a Chadian Bovine

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 20 publications

References 42 publications

Prevalence and distribution of livestock schistosomiasis and fascioliasis in Côte d’Ivoire: results from a cross-sectional survey

Prevalence and distribution of livestock schistosomiasis and fascioliasis in Côte d’Ivoire: results from a cross-sectional survey

Biliary fascioliasis – A rare differential diagnosis of biliary obstruction

Molecular characterization of Fasciola isolates collected from sheep, goats and cattle in Kisumu, Baringo and Narok Counties, Kenya

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