Fascioliasis is a zoonotic disease caused by Fasciola hepatica or F. gigantica. There are two phases of Fasciola infection. In the acute phase, the patient has a wide spectrum of clinical manifestations while the later phase of infection is usually related to inflammatory changes in the bile duct and the mechanical effects of the worm, which can lead to biliary obstruction. The presence of Fasciola inside the biliary system has rarely been reported in the literature. In this report, we presented a case of bile duct obstruction in a 36-year-old male patient undergoing cholecystectomy for gallstones and with a history of worsening jaundice for one month. Extensive laboratory testing failed to clarify the cause of jaundice. Three living liver flukes were subsequently retrieved from the right hepatic duct and later confirmed as F. gigantica by morphology and internal transcribed spacer (ITS2) sequence analysis.

Background: Trichophyton is the common genus in dermatophytes fungi in the world. These fungi secreted virulence enzymes that help them take nutrition from the host. Objectives: 1. To determinate enzymes secretion ability of Trichophyton species isolated from patients, including lipase, phospholipase, and protease. 2. To evaluate the correlation between enzyme secretion ability and dermatomycoses clinical forms. Material and methods: Trichophyton species were isolated from the cultivation samples of dermatophytosis patients. Enzyme production experiments were checked by cultivating fungi into suitable solid agar media. Results: The prevalence of Trichophyton spp. producing protease, lipase, and phospholipase were 96.1%%, 86.2%, and 21.6%, respectively. There were 78.4% of fungal strains that produced both lipase and protease, and 17.6% of fungal strains produced three enzymes. The ability to produce protease of T. rubrum was higher than T. mentagrophytes, and there was no statistically significant difference between lipase and phospholipase activities of these two fungal species. There was no difference between the fungal enzymatic activities and dermatophytosis clinical forms. Conclusion: Trichophyton species isolated from patients attending Hue University of Medicine and Pharmacy Hospital produced more protease and lipase than phospholipase. T. rubrum strains showed higher ability protease produce than T. mentagrophytes. Key words: dermatophytes, Trichophyton spp., fungal enzyme

Background: Parasitic and fungal disease is now a public health problem. Objective: To identify the rate of parasite infection at Hue University of Medicine and Pharmacy Hospital from 2015 to 2019. Materials and methods: Retrospective study, survey of laboratory data at the Department of Parasitology from 2015 to 2019. Results: The rates of serum positive for Fasciola sp., Taenia sp., Strongyloides sp., Toxocara sp. was 48.5%, 21.5%, 32.8%, 67.4%, respectively. Infection rates of hookworm, whipworm, strongyloidiasis, small liver fluke, Taenia sp. was 0.8%, 1.7 %, 1.0%, 2.0%, 1.7% respectively. The rate of vaginal yeast, fungal cutaneous infection was 24.2%, 41.1% respectively. Respiratory fungal infection: Candida albicans 31.7%, C. non albicans 21.6%. The rate of fungal infections of the outer ear canal: Aspergillus 54.6%, C. albicans 2.8%, C. non albicans 13%. Conclusion: These results show that the number of parasitic tests is increasing, which corresponds to the importance role of this kind of diseases to the public health. The Parasitology Department play an important role in order for hospital to improve quality to supplying the good service for public health care Key words: protozoa, Parasite, Fungi, Helminth, ELISA