Molecular cytogenetic analysis of Haemulidae fish (Perciformes): Evidence of evolutionary conservation

Neto, Clóvis Coutinho Motta; Cioffi, Marcelo de Bello; Bertollo, L.A.C.; Molina, Wagner Franco

doi:10.1016/j.jembe.2011.07.014

Cited by 31 publications

(19 citation statements)

References 29 publications

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“…The nucleolus organizer regions (NORs) are considered a highly informative cytogenetic marker in teleosteans (Gornung 2008). The presence of single NORs at pericentromeric region is considered the plesiomorphic condition for several families in Percomorpharia, particularly those characterized by species with 2n=48a (Affonso et al 2001, Motta Neto et al 2011b, as also supported by the present results of 18S rDNA FISH and silver nitrate staining. In the case of L. synagris, the pattern of distribution of ribosomal genes was similar to that previously described for other populations in Brazil (Costa et al 2016).…”

Section: Discussionsupporting

confidence: 82%

“…In general, chromosomal studies in marine Percomorpharia reveal stable karyotypes composed of 2n=48 and a predominance of acrocentric pairs. Indeed, the presence of 24 acrocentric pairs is shared by several species from distinct families of Perciformes (Affonso et al 2001, Accioly and Molina 2007, Cipriano et al 2008, Motta Neto et al 2011a, 2011b, Molina et al 2012, Costa et al 2016. This pattern raises some intriguing questions: (1) how could such a morphologically diversified group evolve without significant chromosomal changes (Brum 1996, Galetti et al 2000, Molina 2007)?…”

Section: Introductionmentioning

confidence: 99%

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Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim

Almeida

Affonso

et al. 2017

CCG

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The weakness of physical barriers in the marine environment and the dispersal potential of fish populations have been invoked as explanations of the apparent karyotype stasis of marine Percomorpha, but several taxa remain poorly studied cytogenetically. To increase the chromosomal data in this fish group, we analyzed cytogenetically three widespread Atlantic species from distinct families: Chaetodipterus faber Broussonet, 1782 (Ephippidae), Lutjanus synagris Linnaeus, 1758 (Lutjanidae) and Rypticus randalli Courtenay, 1967 (Serranidae). The three species shared a karyotype composed of 2n=48 acrocentric chromosomes, single nucleolus organizer regions (NORs) and reduced amounts of centromeric heterochromatin. A single NOR-bearing pair was identified in all species by physical mapping of 18S rDNA while non-syntenic 5S rRNA genes were located at centromeric region of a single pair. The similar karyotypic macrostructure observed in unrelated groups of Percomorpharia reinforces the conservative karyoevolution of marine teleosteans. Nonetheless, the species could be differentiated based on the pair bearing ribosomal cistrons, revealing the importance of microstructural analyses in species with symmetric and stable karyotypes.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Paim

Almeida

Affonso

et al. 2017

CCG

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“…The 5S rDNA localization has been determined in six species of the Haemulidae family, and between two and four chromosomes showed positive signals [54-56]. Therefore, in the Haemulidae family, some species could have suffered translocation events which have led to the gain of an additional 5S rDNA locus.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families

et al. 2012

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BackgroundMolecular and cytogenetic markers are of great use for to fish characterization, identification, phylogenetics and evolution. Multigene families have proven to be good markers for a better understanding of the variability, organization and evolution of fish species. Three different tandemly-repeated gene families (45S rDNA, 5S rDNA and U2 snDNA) have been studied in Plectorhinchus mediterraneus (Teleostei: Haemulidae), at both molecular and cytogenetic level, to elucidate the taxonomy and evolution of these multigene families, as well as for comparative purposes with other species of the family.ResultsFour different types of 5S rDNA were obtained; two of them showed a high homology with that of Raja asterias, and the putative implication of a horizontal transfer event and its consequences for the organization and evolution of the 5S rDNA have been discussed. The other two types do not resemble any other species, but in one of them a putative tRNA-derived SINE was observed for the first time, which could have implications in the evolution of the 5S rDNA. The ITS-1 sequence was more related to a species of another different genus than to that of the same genus, therefore a revision of the Hamulidae family systematic has been proposed. In the analysis of the U2 snDNA, we were able to corroborate that U2 snDNA and U5 snDNA were linked in the same tandem array, and this has interest for tracing evolutionary lines. The karyotype of the species was composed of 2n = 48 acrocentric chromosomes, and each of the three multigene families were located in different chromosome pairs, thus providing three different chromosomal markers.ConclusionsNovel data can be extracted from the results: a putative event of horizontal transfer, a possible tRNA-derived SINE linked to one of the four 5S rDNA types characterized, and a linkage between U2 and U5 snDNA. In addition, a revision of the taxonomy of the Haemulidae family has been suggested, and three cytogenetic markers have been obtained. Some of these results have not been described before in any other fish species. New clues about the genome organization and evolution of the multigene families are offered in this study.

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“…There is an exuberant diversity of shapes and wide body size variety in the Perciformes order, which holds more than 10,000 species [ 19 ]. This morphological diversity contrasts with the high karyotype stability [ 20 ] found in most representatives of this order. They are acrocentric diploid and have fundamental number 2 n = 48 chromosomes, simple ribosomal sites, and little heterochromatin mostly concentrated in the pericentromeric regions [ 21 ].…”

Section: Cryptic Species Detection and Population Structuring In Mmentioning

confidence: 75%

Inferring Diversity and Evolution in Fish by Means of Integrative Molecular Cytogenetics

Artoni

Castro

Jacobina

et al. 2015

The Scientific World Journal

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Fish constitute a paraphyletic and profusely diversified group that has historically puzzled ichthyologists. Hard efforts are necessary to better understand this group, due to its extensive diversity. New species are often identified and it leads to questions about their phylogenetic aspects. Cytogenetics is becoming an important biodiversity-detection tool also used to measure biodiversity evolutionary aspects. Molecular cytogenetics by fluorescence in situ hybridization (FISH) allowed integrating quantitative and qualitative data from DNA sequences and their physical location in chromosomes and genomes. Although there is no intention on presenting a broader review, the current study presents some evidences on the need of integrating molecular cytogenetic data to other evolutionary biology tools to more precisely infer cryptic species detection, population structuring in marine environments, intra- and interspecific karyoevolutionary aspects of freshwater groups, evolutionary dynamics of marine fish chromosomes, and the origin and differentiation of sexual and B chromosomes. The new cytogenetic field, called cytogenomics, is spreading due to its capacity to give resolute answers to countless questions that cannot be answered by traditional methodologies. Indeed, the association between chromosomal markers and DNA sequencing as well as between biological diversity analysis methodologies and phylogenetics triggers the will to search for answers about fish evolutionary, taxonomic, and structural features.

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Molecular cytogenetic analysis of Haemulidae fish (Perciformes): Evidence of evolutionary conservation

Cited by 31 publications

References 29 publications

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Chromosomal stasis in distinct families of marine Percomorpharia from South Atlantic

Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families

Inferring Diversity and Evolution in Fish by Means of Integrative Molecular Cytogenetics

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