Molecular cytogenetic characterization of two murine cancer cell lines derived from salivary gland

Steinacker, Ralf; Liehr, Thomas; Kosyakova, Nadezda; Rinčić, Martina; Azawi, Shaymaa

doi:10.21638/spbu03.2018.403

Cited by 7 publications

(10 citation statements)

References 30 publications

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“…Thus, in this study, the three frequently used murine BC cell lines C-127I, EMT6/P and TA3 Hauschka were for the first time characterized on the molecular cytogenomic level. The feasibility of the applied scheme was shown in several previous studies [ 20 , 36 , 37 , 38 , 39 ].…”

Section: Discussionmentioning

confidence: 78%

Molecular Cytogenomic Characterization of the Murine Breast Cancer Cell Lines C-127I, EMT6/P and TA3 Hauschka

Azawi

Liehr

Rinčić

et al. 2020

IJMS

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Background: To test and introduce effective and less toxic breast cancer (BC) treatment strategies, animal models, including murine BC cell lines, are considered as perfect platforms. Strikingly, the knowledge on the genetic background of applied BC cell lines is often sparse though urgently necessary for their targeted and really justified application. Methods: In this study, we performed the first molecular cytogenetic characterization for three murine BC cell lines C-127I, EMT6/P and TA3 Hauschka. Besides fluorescence in situ hybridization-banding, array comparative genomic hybridization was also applied. Thus, overall, an in silico translation for the detected imbalances and chromosomal break events in the murine cell lines to the corresponding homologous imbalances in humans could be provided. The latter enabled a comparison of the murine cell line with human BC cytogenomics. Results: All three BC cell lines showed a rearranged karyotype at different stages of complexity, which can be interpreted carefully as reflectance of more or less advanced tumor stages. Conclusions: Accordingly, the C-127I cell line would represent the late stage BC while the cell lines EMT6/P and TA3 Hauschka would be models for the premalignant or early BC stage and an early or benign BC, respectively. With this cytogenomic information provided, these cell lines now can be applied really adequately in future research studies.

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Section: Discussionmentioning

confidence: 78%

Molecular Cytogenomic Characterization of the Murine Breast Cancer Cell Lines C-127I, EMT6/P and TA3 Hauschka

Azawi

Liehr

Rinčić

et al. 2020

IJMS

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“…mFISH using whole chromosome painting probes revealed the general characteristics of the cell lines, like the ploidy, clonal and nonclonal changes as well as numerical and intrachromosomal structural aberrations. By mcb interchromosomal alterations as duplications, deletions or inversions, and chromosomal breakpoints involved could be uncovered, as previously reported [23][24][25][26][27][28]. The aCGH data was aligned with the FISH results and also used to determine breakpoints of unbalanced rearrangements (Additional file 1: Table 1).…”

Section: Discussionmentioning

confidence: 97%

Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

et al. 2020

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Background Malignant mesothelioma (MM) is a rare aggressive cancer primary located in pleura and lung. MMs can be divided into biphasic, epithelioid and sarcomatoid subtypes. In majority of cases MMs are induced by asbestos fiber exposure. As latency period after asbestos exposure ranges between ~ 10 and 60 years MMs are mainly observed in elder people. Human MM, being a rare tumor type, lacks detailed cytogenetic data, while molecular genetic studies have been undertaken more frequently. However, murine MM cell lines are also regularly applied to get more insight into MM biology and to test new therapy strategies. Results Here the murine MM cell lines AB1, AB22 and AC29 were studied by molecular cytogenetics and molecular karyotyping. Interestingly, yet there were no genetic or genomic studies undertaken for these already in 1992 established cell lines. The obtained data on genomic imbalances in these murine cell lines was translated into the human genome as previously reported based on human and murine genomic browsers. Conclusions It turned out that all three cell lines showed high similarities in copy number variants as observed typically in human MM. Also, all three cell lines were most similar to human epithelioid MMs, and should be used as models therefore.

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“…Accordingly, especially murine tumor cell lines are favorite model systems to perform basic research studies, as well as such towards testing of new medications [ 8 , 33 ]. Our group characterized already several murine tumor cell lines by the same test strategy as applied here, and thus delivered previously not available but urgently necessary genetic basic data, including chromosome numbers, observable breakpoints and copy number changes [ 1 , 16 , 17 , 19 , 34 , 35 ]. Here a commercially available murine BC-cell line was characterized for the first time, cell line JC, which was yet rarely used in research.…”

Section: Discussionmentioning

confidence: 99%

“…JC cell line presents a near-tetraploidy karyotype, which is typically observable, especially in human [ 36 ], however, only present in about 50% of murine tumor cell lines [ 1 , 16 , 17 , 19 , 34 , 35 ]. Polyploidy promotes malignant transformation of mammary cells and increases cell resistance to drugs [ 36 ]; still, it is also a known adaptation of cells to cell culture conditions.…”

Section: Discussionmentioning

confidence: 99%

Cytogenomic characteristics of murine breast cancer cell line JC

2021

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Background Breast cancer (BC), one of the most frequent human tumors, is genetically and histologically heterogeneous. Treatment options can be adapted according to BC subtype. Still, research is necessary to characterize BC biology better and to study potential new treatment options. Murine BC-cell lines can be used as model systems in this respect. Results Here for the first time murine BC-cell line JC was cytogenomically characterized as being complex rearranged and near-tetraploid. Multicolor banding and array comparative genomic hybridization were applied and the result was in silico translated to the human genome. Conclusions Even though being commercially available, cell line JC was yet not much included in BC-research, most likely due to a lack of cytogenomic data. Thus, here comprehensive data is provided on chromosomal aberrations, genomic imbalances and involved breakpoints of JC cell line. Also JC could be characterized as a model for BC of luminal B type, basal-like tumor rather than for luminal A type.

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Molecular cytogenetic characterization of two murine cancer cell lines derived from salivary gland

Cited by 7 publications

References 30 publications

Molecular Cytogenomic Characterization of the Murine Breast Cancer Cell Lines C-127I, EMT6/P and TA3 Hauschka

Molecular Cytogenomic Characterization of the Murine Breast Cancer Cell Lines C-127I, EMT6/P and TA3 Hauschka

Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

Cytogenomic characteristics of murine breast cancer cell line JC

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