Molecular cytogenetic identification of a wheat – <i>Thinopyrum ponticum</i> substitution line with stripe rust resistance

Wang, Yanzhen; Chen, Chunhuan; Wang, Changyou; Zhang, Aicen; Peng, Nana; Wang, Yajuan; Zhang, Hong; Liu, Xinlun; Ji, Wanquan

doi:10.1139/gen-2017-0099

Cited by 22 publications

(13 citation statements)

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“…The FISH probes, Oligo-pSc119.2 and Oligo-pTa535, can accurately distinguish all 42 common wheat chromosomes based on the standard FISH karyotypes in mitotic cells [39,40]. Many wheat-derived lines including substitution and translocation lines have been identified using this method [41][42][43]. In addition, structural changes in wheat chromosomes can be detected by FISH.…”

Section: Discussionmentioning

confidence: 99%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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Background: Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carries many outstanding agronomic traits, therefore is a valuable resource for wheat genetic improvement. Wheat-P. huashanica translocation lines are important intermediate materials for wheat breeding and studying the functions of alien chromosomes. However, powdery mildew resistance in these translocation lines has not been reported previously.Results: This study developed a novel wheat-P. huashanica translocation line TR77 by selecting a F 7 progeny from the cross between heptaploid hybrid H8911 (2n = 7x = 49, AABBDDNs) and durum wheat line Trs-372. Chromosome karyotype of 2n = 42 = 21II was observed in both mitotic and meiotic stages of TR77. Genomic in situ hybridization analysis identified two translocated chromosomes that paired normally at meiosis stage in TR77. Molecular marker analysis showed that part of chromosome 5D was replaced by part of alien chromosome fragment 5Ns. It meant replacement made part 5DL and part 5NsL·5NsS existed in wheat background, and then translocation happened between these chromosomes and wheat 3D chromosome. Fluorescence in situ hybridization demonstrated that TR77 carries dual translocations: T3DS-5NsL·5NsS and T5DL-3DS·3DL. Analysis using a 15 K-wheat-SNP chip confirmed that SNP genotypes on the 5D chromosome of TR77 matched well with these of P. huashanica, but poorly with common wheat line 7182. The translocation was physically located between 202.3 and 213.1 Mb in 5D. TR77 showed longer spikes, more kernels per spike, and much better powdery mildew resistance than its wheat parents: common wheat line 7182 and durum wheat line Trs-372.(Continued on next page)

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Section: Discussionmentioning

confidence: 99%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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“…Root tip cells and PMCs of CH10A5 from the same plant were observed for three growing seasons (2016-2019) in a total of 34 plants. Root tip processing procedures were performed as described in Zheng et al (2006), using a mixture of cellulase (R-10, Yakult Japan) and pectinase (Y-23, Yakult Japan) for cell enzymatic hydrolysis (Zhu et al, 2017). Young spikes (Zadoks stage 45) were sampled, and anthers at meiosis metaphase I (MI) were fixed in Carnoy fixative (a 6:3:1 ethanol-chloroform-acetic acid mixture) for at least 2 days and stained with a 1% (w/v) aceto-carmine solution prior to in situ hybridization of the PMCs as described in Yang et al (2014).…”

Section: Cytological Stability Assessmentmentioning

confidence: 99%

“…Despite the development of numerous wheat-Th. ponticum chromosome lines, addition or substitution lines of homoeologous group one are rarely reported (Mo et al, 2017;Zhu et al, 2017;Mago et al, 2019;Wang et al, 2019), and only a few stripe rust and powdery mildew resistance genes have been introgressed into cultivated wheat varieties (Li et al, 2003;Zhan et al, 2014;Hou et al, 2016). The resistance genes of Th.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Analysis and Molecular Marker Development for a New Wheat–Thinopyrum ponticum 1Js (1D) Disomic Substitution Line With Resistance to Stripe Rust and Powdery Mildew

et al. 2020

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Thinopyrum ponticum (2n = 10x = 70), a member of the tertiary gene pool of wheat (Triticum aestivum L.), harbors many biotic and abiotic stress resistance genes. CH10A5, a novel disomic substitution line from a cross of T. aestivum cv. 7182 and Th. ponticum, was characterized by cytogenetic identification, in situ hybridization, molecular marker analysis, and morphological investigation of agronomic traits and disease resistance. Cytological observations showed that CH10A5 contained 42 chromosomes and formed 21 bivalents at meiotic metaphase I. Genome in situ hybridization (GISH) analysis indicated that two of its chromosomes came from the J s genome of Th. ponticum, and wheat 15K array mapping and fluorescence in situ hybridization (FISH) revealed that chromosome 1D was absent from CH10A5. Polymorphic analysis of molecular markers indicated that the pair of alien chromosomes belonged to homoeologous group one, designated as 1J s. Thus, CH10A5 was a wheat-Th. ponticum 1J s (1D) disomic substitution line. Field disease resistance trials demonstrated that the introduced Th. ponticum chromosome 1J s was probably responsible for resistance to both stripe rust and powdery mildew at the adult stage. Based on specific-locus amplified fragment sequencing (SLAF-seq), 507 STS molecular markers were developed to distinguish chromosome 1J s genetic material from that of wheat. Of these, 49 STS markers could be used to specifically identify the genetic material of Th. ponticum. CH10A5 will increase the resistance gene diversity of wheat breeding materials, and the markers developed here will permit further tracing of heterosomal chromosome fragments in the future.

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“…This is necessary to improve wheat disease resistance, enable screening of large germplasm resources, and enable the transfer and polymerization of resistance genes. It is an increasingly important approach for enriching common wheat with beneficial genes derived from related species by distant hybridization [3][4][5]. Many powdery mildew resistance genes are derived from wild relatives of wheat, and have been introduced to common wheat by additions, substitutions, and translocations of chromosomes.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

Wang

Long

Wang

et al. 2020

IJMS

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Aegilops geniculata Roth has been used as a donor of disease-resistance genes, to enrich the gene pool for wheat (Triticum aestivum) improvement through distant hybridization. In this study, the wheat–Ae. geniculata alien disomic substitution line W16998 was obtained from the BC1F8 progeny of a cross between the common wheat ‘Chinese Spring’ (CS) and Ae. geniculata Roth (serial number: SY159//CS). This line was identified using cytogenetic techniques, analysis of genomic in situ hybridization (GISH), functional molecular markers (Expressed sequence tag-sequence-tagged site (EST–STS) and PCR-based landmark unique gene (PLUG), fluorescence in situ hybridization (FISH), sequential fluorescence in situ hybridization–genomic in situ hybridization (sequential FISH–GISH), and assessment of agronomic traits and powdery mildew resistance. During the anaphase of meiosis, these were evenly distributed on both sides of the equatorial plate, and they exhibited high cytological stability during the meiotic metaphase and anaphase. GISH analysis indicated that W16998 contained a pair of Ae. geniculata alien chromosomes and 40 common wheat chromosomes. One EST–STS marker and seven PLUG marker results showed that the introduced chromosomes of Ae. geniculata belonged to homoeologous group 7. Nullisomic–tetrasomic analyses suggested that the common wheat chromosome, 7A, was absent in W16998. FISH and sequential FISH–GISH analyses confirmed that the introduced Ae. geniculata chromosome was 7Mg. Therefore, W16998 was a wheat–Ae. geniculata 7Mg (7A) alien disomic substitution line. Inoculation of isolate E09 (Blumeria graminis f. sp. tritici) in the seedling stage showed that SY159 and W16998 were resistant to powdery mildew, indeed nearly immune, whereas CS was highly susceptible. Compared to CS, W16998 exhibited increased grain weight and more spikelets, and a greater number of superior agronomic traits. Consequently, W16998 was potentially useful. Germplasms transfer new disease-resistance genes and prominent agronomic traits into common wheat, giving the latter some fine properties for breeding.

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Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Cited by 22 publications

References 50 publications

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Cytogenetic Analysis and Molecular Marker Development for a New Wheat–Thinopyrum ponticum 1Js (1D) Disomic Substitution Line With Resistance to Stripe Rust and Powdery Mildew

Characterization and Evaluation of Resistance to Powdery Mildew of Wheat–Aegilops geniculata Roth 7Mg (7A) Alien Disomic Substitution Line W16998

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