SummaryThe karyotype of Bellevalia romana L., which is composed of two long metacentric chromosomes (chromosome 1), two short subtelocentric chromosomes (chromosome 2), and four short submetacentric chromosomes (chromosomes 3 and 4), were analyzed by several techniques. C-bands, CMA 3 bands, Ag-staining bands, and FISH signals of 18S rDNA appeared at the same sites on the end of an arm of chromosomes 1 and 3. A large FISH signal with 5S rDNA was localized at the end of the long arm of chromosome 2, and a small signal appeared at the interstitial region of the long arm of chromosome 1. When compared with a previous report, there were differences in the CMA 3 band on chromosome 2 and a small 5S rDNA signal on chromosome 1.