Molecular detection and characterization of tick-borne parasites in goats and ticks from Thailand

Aung, Aung; Kaewlamun, Winai; Narapakdeesakul, Duriyang; Poofery, Juthathip; Kaewthamasorn, Morakot

doi:10.1016/j.ttbdis.2022.101938

Cited by 13 publications

(9 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection rate (38.64%) of Anaplasma spp. in the current study was higher than what was observed in goats from Bangladesh (15.75%) [ 33 ], Thailand (13.50%) [ 34 ], Pakistan (7.80%) [ 35 ], and South Korea (7.62%) [ 36 ], but lower than in goats from China (58.50%) [ 37 ]. The relatively high detection rates may be due to several factors related to the climate, environment, host susceptibility, vector population density, and management production systems [ 3 , 27 , 38 ].…”

Section: Discussioncontrasting

confidence: 78%

“…We also obtained an isolate phylogenetically distinct from other analyzed sequences (MW786652; 98.75% identity with Theileria sequences in GenBank), which may be a new Theileria sp., although more studies are needed to verify this claim. On the other hand, T. luwenshuni, which is a common species in goats reported from nearby Southeast Asian countries, namely, Thailand [ 34 , 53 , 54 , 55 ], Myanmar [ 56 ], and Vietnam [ 50 , 57 , 58 ], was not confirmed in the obtained sequences.…”

Section: Discussionmentioning

confidence: 74%

“…The most likely vector of piroplasmas and Anaplasma is the ubiquitous R. microplus ticks, which was found infesting goats in Bulacan province [ 66 ]. The tick species R. microplus and Haemaphysalis bispinosa infesting goats from neighboring countries Thailand [ 34 , 67 ] and Malaysia [ 68 ] were also confirmed to be carrying T. luwenshuni . Similarly, R. sanguineus sensu lato (s.l.)…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

et al. 2022

View full text Add to dashboard Cite

Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites Babesia spp. and Theileria spp., while anaplasmosis is caused by bacterial Anaplasma spp. In the Philippines, the presence of Babesia, Theileria, and Anaplasma has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for Babesia/Theileria and Anaplasma. Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and Anaplasma using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, Babesia ovis was detected in six samples (1.52%). Representative Babesia/Theileria sequences shared 89.97–97.74% identity with each other and were most closely related to T. orientalis, T. annulata, and Theileria spp. Meanwhile, Anaplasma 16SrRNA sequences were related to A. odocoilei, A. platys, and A. phagocytophilum. This is the first molecular identification of B. ovis, Theileria spp., and Anaplasma spp. in goats from the Philippines.

show abstract

Section: Discussioncontrasting

confidence: 78%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

et al. 2022

View full text Add to dashboard Cite

show abstract

“…To our knowledge, A. ovis has never been detected in goats in Egypt. Recently, some studies have reported goats infected with A. ovis in Iraq [ 48 ], Thailand [ 49 ], and Bangladesh [ 50 ]. Likewise, we found that buffaloes were positive for A. platys (GenBank: OL721670).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

et al. 2022

View full text Add to dashboard Cite

Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. A total of 234 blood samples and 95 adult ticks were collected from animal hosts (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, and 8 horses) from six provinces of Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, and Matruh). Blood and tick samples were investigated by polymerase chain reaction coupled with sequencing targeting 18S and 16S RNA genes for Piroplasma sp. and anaplasmataceae, respectively. Statistical analysis was conducted on the potential epidemiological factors. Of the 234 animals examined, 54 (23.08%) were positive for pathogens DNA distributed among the six provinces, where 10 (4.27%) were positive for Piroplasma sp., 44 (18.80%) for anaplasmataceae, and 5 (2.14%) were co-infected. Co-infections were observed only in cattle as Theileria annulata and Anaplasma marginale plus Babesia bigemina, A. marginale plus B. bigemina, and T. annulata plus B. bigemina. Piroplasmosis was recorded in cattle, with significant differences between their prevalence in their tick infestation factors. Animal species, age, and tick infestation were the potential risk factors for anaplasmosis. All ticks were free from piroplasms, but they revealed high prevalence rates of 72.63% (69/95) with anaplasmataceae. We identified T. annulata, B. bigemina, and A. marginale in cattle; A. platys in buffaloes; A. marginale and A. ovis in sheep; for the first time, A. ovis in goats; and Ehrlichia sp. in Rhipicephalus annulatus ticks. Our findings confirm the significant prevalence of piroplasmosis and anaplasmosis among subclinical and carrier animals in Egypt, highlighting the importance of the government developing policies to improve animal and public health security.

show abstract

“…It may result from multi‐species Anaplasma mixed infection, as recently shown by researchers such as Aung et al. (2022) and Ochirkhuu et al. (2017).…”

Section: Discussionmentioning

confidence: 79%

Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences

Abbas Zadeh,

Hamidinejat,

Nikvand

et al. 2024

Veterinary Medicine & Sci

View full text Add to dashboard Cite

BackgroundAnaplasma ovis (A. ovis) is the predominant causative agent of anaplasmosis in goats and sheep in most tropical and subtropical regions of the world. However, there is considerable variation in reported infection rates, breed susceptibility, and controversial findings regarding the haemolytic effects of A. ovis infection in goats.ObjectivesThus, we investigated the molecular and haematological aspects of A. ovis infection in goats from Ahvaz city.MethodsOne hundred and fifty apparently healthy goats (74 blacks and 76 Najdi goats) were randomly sampled from six flocks in the Ahvaz suburb during ticks’ activity season. Haematological evaluation, smear microscopic (SM) examination and PCR assay were performed to assess A. ovis infection. Additionally, the percentage of parasitemia was determined from blood smears.ResultsSM examination revealed that 25.7% of the goats displayed erythrocyte Anaplasma‐like inclusion bodies. PCR analysis indicated that 54% of the goats were positive for A. ovis infection (44.6% of blacks and 63.2% of Najdi goats). No significant difference in haematological values was observed between healthy and infected goats based on PCR testing. However, a significant difference in haematological indices was observed between the group with parasitemia level of 0.01–0.02% (SM and PCR positive) compared to the healthy goats (SM and PCR negative), particularly concerning Hb, PCV and RBC count (p < 0.01).ConclusionsWhen the parasitemia exceeds 0.01%, A. ovis infection may disrupt haematological parameters in infected goats. The high prevalence of A. ovis infection (54%) among the studied goats underscores the importance of giving special attention to implementing necessary measures for disease control in the Ahvaz suburb.

show abstract

Molecular detection and characterization of tick-borne parasites in goats and ticks from Thailand

Cited by 13 publications

References 48 publications

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

Molecular and haematological investigations on Anaplasma ovis infection in goats in Ahvaz, Iran: Insights into infection rate, haemolytic effect and breed influences

Contact Info

Product

Resources

About