Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks

Ochirkhuu, Nyamsuren; Konnai, Satoru; Odbileg, Raadan; Odzaya, Battogtokh; Shura, Gansukh; Shiro, Motoo; Ohashi, Kazuhiko

doi:10.1007/s00705-016-2890-z

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…: genotyping was not performed. References (I): [80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99]; (II): [47,100,101,102,103,104]; (III): [49]; (IV): [37,49,105,106,107,108,109]; (V): [110,111,112]; (VI): [113]. In [49], [83], [85] and [89] the year of virus isolation was not displayed in the study.…”

Section: Figurementioning

confidence: 99%

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Yeşilbağ

Alpay

Becher

2017

Viruses

208

250

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Bovine viral diarrhea virus (BVDV) is a globally-distributed agent responsible for numerous clinical syndromes that lead to major economic losses. Two species, BVDV-1 and BVDV-2, discriminated on the basis of genetic and antigenic differences, are classified in the genus Pestivirus within the Flaviviridae family and distributed on all of the continents. BVDV-1 can be segregated into at least twenty-one subgenotypes (1a–1u), while four subgenotypes have been described for BVDV-2 (2a–2d). With respect to published sequences, the number of virus isolates described for BVDV-1 (88.2%) is considerably higher than for BVDV-2 (11.8%). The most frequently-reported BVDV-1 subgenotype are 1b, followed by 1a and 1c. The highest number of various BVDV subgenotypes has been documented in European countries, indicating greater genetic diversity of the virus on this continent. Current segregation of BVDV field isolates and the designation of subgenotypes are not harmonized. While the species BVDV-1 and BVDV-2 can be clearly differentiated independently from the portion of the genome being compared, analysis of different genomic regions can result in inconsistent assignment of some BVDV isolates to defined subgenotypes. To avoid non-conformities the authors recommend the development of a harmonized system for subdivision of BVDV isolates into defined subgenotypes.

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Section: Figurementioning

confidence: 99%

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Yeşilbağ

Alpay

Becher

2017

Viruses

208

250

View full text Add to dashboard Cite

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“…BVDV infection has a global distribution and affects animal health and reproductive performance, resulting in significant economic losses. Recently, the prevalence study of BVDV in cows has been determined worldwide (Amelung et al 2018;Aragaw et al 2018;Evans et al 2018;Khodakaram-Tafti and Farjanikish 2017;Kumar et al 2018;Ochirkhuu et al 2016). In China, the disease was firstly reported in the 1980s of the last century Fulton 2009), and most of the investigations concerning BVDV infection were seldom published internationally (Deng et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of bovine viral diarrhea virus in dairy cattle herds in eastern China

Hou

Zhao

Wang

et al. 2018

Trop Anim Health Prod

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Bovine viral diarrhea virus (BVDV) is a worldwide spreading pestivirus affecting cattle and other ruminants; however, there have been few reports on epidemiologic investigation of BVDV in eastern China. In this study, bulk tank milk from 36 herds of dairy cattle in eastern China was submitted to serological investigations, 77.8% of herds was BVDV antibody positive. Individual animal status in two herds was further investigated collecting blood samples, the positive ratio was 49.74% and 24.64%, and the average positive ratio of calves, heifers, and lactating cows was 15.94%, 40.16%, and 41.7%, respectively. Moreover, clinical survey was carried out among 8170 dairy cattle from 36 herds, for diarrhea syndrome, respiratory problems and reproductive failure, and pathogens of all clinical cattle were further investigated. The results showed that BVDV was one of the main pathogen, which infected animals combining with various other viruses. Then, nine BVDV strains were isolated; phylogenetic analysis showed that BVDV subtypes currently circulating in eastern China were BVDV 1a and BVDV 1c. In addition, out of 377 cows tested, the 1.86% detected positive to the BVDV antigen. This study provided the foundation of further study on vaccination and control strategies of BVDV in eastern China.

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“…The largest number of subtypes (up to 21) was found in cattle in Italy [13] and China [14]. BVDV-2 in cattle was found in the USA [15], Canada [16], Brazil [17], Argentina [18], Uruguay [19], Germany [20], Slovakia [21], Italy [22], South Korea [23], Japan [24] and Mongolia [25]. This type, which is considered more virulent, is divided into six subtypes (2a-2f) [26] and prevails in the USA and Canada (up to 50 % of all isolated strains) [1].…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism of the Bovine Viral Diarrhea Viruses in Big Dairy Farms in Siberia

Koteneva¹,

Nefedchenko²,

Glotova³

et al. 2018

s-h. biol.

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A b s t r a c tBovine viral diarrhea is a widespread disease in the Russian Federation and causes significant economic damage to dairy cattle, especially under intensive commercial farming. The seropositivity of livestock in various Russian regions reaches 65-100 %. The disease is caused by two different virus species, BVDV-1 and BVDV-2, of which the latter is considered more virulent. The persistently infected (PI) animals which are a constant endogenous source of the virus in the herd play a major role in maintaining the stationary trouble of the farms. Short-term sources of the pathogen are transitively infected (TI) animals in which disease proceeds in acute form. Genetic structure of viruses circulating in local livestock populations gives necessary information about the evolution, geography and pathways of the pathogen. However, studies on the genetic polymorphism of viruses in Russia are not enough. This paper is the first to report on a phylogenetic analysis of two types of viral diarrhea virus, isolated from animals of foreign and domestic origin with different clinical manifestations of the disease. These data show the prevalence of BVDV-1b in PI and TI animals, as well as BVDV-2b and BVDV-2c circulation among Siberian dairy complexes (note, BVDV-2b and BVDV-2c in animals of foreign and domestic origin are detected in Russia for the first time). Phylogenetic analysis of viruses circulating among PI and TI of highly productive dairy cattle was based on a comparison of conserved region of viral genome (5´-UTR) using reverse transcriptase PCR (RT-PCR) method. Studies were conducted in five regions of Western and Eastern Siberia: Tyumen, Novosibirsk, Irkutsk regions, the Krasnoyarsk Territory and Northern Kazakhstan on big dairy farms with a population of 800 or more milking cows with an average annual productivity of 7,000-10,000 liters, where at the time of the research vaccination was not carried out or inactivated vaccines were used. The imported livestock was kept mainly in the Tyumen region and the Republic of Kazakhstan. Biomaterial (blood, serum, nasal discharge, lymphoid organs, lungs, vaginal discharges) was collected from clinically healthy persistently infected animals, transitively infected animals with reproductive disorders and respiratory syndrome, as well as aborted fetuses. A total of 479 samples were examined. According to our findings, two BVDV species circulate among the PI and TI animals on the big dairy farms. The phylogenetic analysis reveals seven subtypes of BVDV-1, i.e. 1a (5 %), 1b (35 %), 1c (5 %), 1d (10 %), 1f (20 %), 1i (5 %), 1p (5 %) and two subtypes of BVDV-2, i.e. 2b (10 %) and 2c (5 %). Taking into account the fact that the strategy of livestock breeding is changing in Russia, the number of dairy mega farms" is increasing, which receive animals with different infectious status from many sources. Thence, the study of the genetic polymorphism of the virus is topical. Comparison of data on the origin of animals with the results of phylogenetic analyses can help in determina...

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Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks

Cited by 7 publications

References 28 publications

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Variability and Global Distribution of Subgenotypes of Bovine Viral Diarrhea Virus

Prevalence of bovine viral diarrhea virus in dairy cattle herds in eastern China

Genetic Polymorphism of the Bovine Viral Diarrhea Viruses in Big Dairy Farms in Siberia

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