Molecular detection and identification of alfalfa mosaic virus (AMV) on pepper cultivated in open fields in R. Macedonia

Abstract

Alfalfa mosaic virus (AMV) is one of the most distributed and economically important plant viruses in pepper in R. Macedonia. Serological detection of AMV in eight important pepper production regions in R. Macedonia and molecular identification of a representative isolate were performed. The virus detection of AMV was conducted using DAS-ELISA method. In order to make molecular detection, RT-PCR was performed. Phylogenetic analysis was conducted, based on the partial sequences of the coat protein gene. A genetic relationship of the Macedonian isolate KUA7-2013 gained in this study was compared with 29 AMV isolates from other parts of the world. High level of nucleotide (92-94.4%) and amino acid identities (91.9-97.1%) were determined. In the constructed phylogenetic tree, the Macedonian AMV isolate was clustered in group II together with isolates from France and Great Britain. In this study, for the first time in R. Macedonia, an isolate of AMV was identified at the molecular level.

Coat protein of alfalfa mosaic alfamovirus (AMV) from Türkiye: genetic inference and in silico docking analysis for potential antiphytoviral purposes

“…Through the use of phylogenetic analysis based on the AMV coat protein of AMV isolates from different regions of the world, several studies have reported on genetic relationships between AMV isolates as well as the differentiation between AMV subgroups (Bergua et al, 2014;Bandjo-Oreshkovikj et al, 2017;Trucco et al, 2022;Wang et al, 2023). Although numerous studies have been conducted on AMV in various regions of Türkiye, few of them have investigated the phylogenetic relationships of this pathogen.…”

Molecular detection of resistance‐breaking strain Cucumber mosaic virus (rbCMV) (Cucumovirus; Bromoviridae) on resistant commercial pepper cultivars in Turkey

“…Peppers are reported to be naturally infected by more than 10 plant viruses (Kenyon et al, 2014). Patato virus Y (PVY), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Cucumber mosaic virus (CMV) Tomato spotted wilt virus (TSWV), and Alfalfa mosaic virus (AMV) are the most widespread viruses (Almási et al, 2020; Bandjo‐Oreshkovikj et al, 2017; Kitajima et al, 2018; Li et al, 2020; Moury et al, 2017). Symptoms such as mosaic, yellowing, fruit deformations, green mottling, vein clearing, leaf distortion, and leathery leaf appearance are linked to viral agents.…”