Molecular detection and identification of phytoplasmas in a novel 16SrI subgroup in sunflowers and cocklebur weeds

Zhang, Lei; Sun, Pu; Ma, Qiang; Xu, Xiaozhao; Zhou, Hongyou; Li, Zhengnan

doi:10.1007/s42161-018-00228-9

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Similarity coefficients derived from virtual RFLP analysis of 16S rRNA genes of strain PLH102-1 and other 16SrI subgroups were less than or equal to 0.97, the threshold for a new subgroup delineation [ 26 ]. Therefore, strain PLH102-1 was designated as a new subgroup 16SrI-AO ( Table 2 [ 10 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ], some of previously reported 16SrI subgroups were reassigned due to duplication).…”

Section: Resultsmentioning

confidence: 99%

“…( b ) This is a re-designation of a subgroup pattern (16SrI-AI) established by Perez-Lopez et al [ 40 ]. The reason for the re-designation is that the subgroup letter 16SrI-AI had been previously published by Zhang et al [ 47 ]. ( c ) This is re-designation of the subgroup 16SrI-AG reported by Santos-Cervantes et al [ 36 ] as the subgroup letter 16SrI-AG (MH279522) had already been assigned to NS1P1cB, BS, blueberry stunt, Canada.…”

Section: Figurementioning

confidence: 99%

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Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

et al. 2021

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Phytoplasmas are obligate transkingdom bacterial parasites that infect a variety of plant species and replicate in phloem-feeding insects in the order Hemiptera, mainly leafhoppers (Cicadellidae). The insect capacity in acquisition, transmission, survival, and host range directly determines the epidemiology of phytoplasmas. However, due to the difficulty of insect sampling and the lack of follow-up transmission trials, the confirmed phytoplasma insect hosts are still limited compared with the identified plant hosts. Recently, quantitative polymerase chain reaction (qPCR)-based quick screening of 227 leafhoppers collected in natural habitats unveiled the presence of previously unknown phytoplasmas in six samples. In the present study, 76 leafhoppers, including the six prescreened positive samples, were further examined to identify and characterize the phytoplasma strains by semi-nested PCR. A total of ten phytoplasma strains were identified in leafhoppers from four countries including South Africa, Kyrgyzstan, Australia, and China. Based on virtual restriction fragment length polymorphism (RFLP) analysis, these ten phytoplasma strains were classified into four distinct ribosomal (16Sr) groups (16SrI, 16SrIII, 16SrXIV, and 16SrXV), representing five new subgroups (16SrI-AO, 16SrXIV-D, 16SrXIV-E, 16SrXIV-F, and 16SrXV-C). The results strongly suggest that the newly identified phytoplasma strains not only represent new genetic subgroup lineages, but also extend previously undiscovered geographical distributions. In addition, ten phytoplasma-harboring leafhoppers belonged to seven known leafhopper species, none of which were previously reported insect vectors of phytoplasmas. The findings from this study provide fresh insight into genetic diversity, geographical distribution, and insect host range of phytoplasmas. Further transmission trials and screening of new potential host plants and weed reservoirs in areas adjacent to collection sites of phytoplasma harboring leafhoppers will contribute to a better understanding of phytoplasma transmission and epidemiology.

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Section: Resultsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

et al. 2021

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“…The reason of the unspecific digestion was kept unknown. It is known that phytoplasmas of 16SrI are the main group occurred in China (Li, Zhang, Liu, et al, 2012), and various subgroups and novel subgroups were discovered (Li, Zhang, Liu, et al, 2012; Li et al., 2014; Luan et al., 2018; Tseng et al., 2016; Zhang et al., 2019). In this work, we extended the queue of 16SrI subgroups with another two novel subgroups of 16SrI‐AK and ‐AL.…”

Section: Discussionmentioning

confidence: 99%

“…With the DNA as template, attempts to amplify partial 16S rRNA gene and rp genes were performed using published primers in polymerase chain reaction (PCR). In the PCR, TaKaRa Taq (TaKaRa) was the choice of DNA polymerase, and a total volume of 25 μl was set up as described previously (Zhang et al., 2019). To generate 16S rRNA gene fragments (F2nR2 region), the primer pair P1/P7 (Deng & Hiruki, 1991; Schneider, Seemüller, Smart, & Kirkpatrick, 1995) was used in a first run of PCR, followed by a second run of PCR using primer pair R16F2n/R16R2 (Gundersen & Lee, 1996); the PCR cycling conditions were set up as described previously by Nejat, Sijam, Abdullah, Vadamalai, and Dickinson (2010) and Lee et al.…”

Section: Methodsmentioning

confidence: 99%

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Sun

Zhang

2020

Forest Pathology

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In September 2019, two diseased plants of Campsis grandiflora showing the main symptom of witches' ‐broom (CgWB) were found in a nursery garden in Yangling, Shaanxi province, China. Partial 16S ribosomal RNA (F2nR2 region) and ribosomal protein (rp) genes of phytoplasmas were generated from the symptomatic plants by PCR amplification, and phytoplasma bodies were observed in the sieve tube elements of the CgWB samples under a transmission electron microscope, indicating phytoplasma infection in the two CgWB plants. Restriction fragment length polymorphism analysis of the F2nR2 region and similarity coefficient results suggested that the two associated phytoplasmas belong to two novel subgroups of 16SrI (aster yellows) group, designated as AK and AL. On the reconstructed phylogenetic trees based on F2nR2 regions and rp genes of phytoplasmas, respectively, the CgWB‐associated phytoplasmas clustered together with members of 16SrI subgroups. This was the first record of phytoplasmas infecting C. grandiflora worldwide.

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Molecular detection and identification of Brassica juncea phyllody and witches’ broom-associated phytoplasmas of group 16SrVI-A in Inner Mongolia, China

et al. 2019

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Molecular detection and identification of phytoplasmas in a novel 16SrI subgroup in sunflowers and cocklebur weeds

Cited by 5 publications

References 20 publications

Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Molecular detection and identification of Brassica juncea phyllody and witches’ broom-associated phytoplasmas of group 16SrVI-A in Inner Mongolia, China

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