Molecular detection and identification of Enterococcus faecium isolated from dental root canals

Abstract

Enterococci are usually encountered and predominate in oral infections, especially those associated with dental root canal infections of necrotic pulp and periodontitis. This study aimed to detect and identify Enterococcus faecium isolated from infected root canals, using polymerase chain reaction ( PCR). Thirty samples were collected from patients with necrotic pulp, infected root canals, and endodontic treatment failure, attending the Conservative Treatment Department, College of Dentistry, Mosul University, Dental Teaching Hospital. The samples were obtained by inserting sterile paper points into the root canals and transferred in brain heart infusion broth vials to be inoculated in a selective M-Enterococcus Agar Base . Twenty five isolates that belong to the genus Enterococcus were recognized by traditional culture methods and biochemical tests. Then, DNA extractions of these isolates were carried out for identification with PCR by the amplification of ddI (D-Ala-D-Ala Ligase) chromosomal genes of Enterococcus faecium. Among the 25 isolates, twenty (80%) were identified to the level of Enterococcus faecium by traditional culture methods and biochemical tests, in comparison to 17 (68%) identified by molecular identification. The PCR products for the specific primer produced bands on agarose gel at the position of 658bp. The study showed that the use of PCR with primers for the E. faecium ddI gene may be the most accurate method for rapid identification of Enterococci. Molecular
 identification of Enterococcus spp. revealed a significant role of E. feacium in root canal infections. Also, the detection of ddI gene using PCR provides a definitive target that could be used for the detection of E. faecium from clinical samples.

The Effect of Different Obturation Techniques in Primary Teeth on the Apical Microleakage using Endoflas: A Comparative In Vitro Study

“…The ability to remove the infectious micro-organisms and create good sealing by the filling material is crucial for efficient root canal treatment [35][36][37][38][39]. Unlike obturation in permanent teeth, performing hermitic seal root canal filling in primary teeth is challenging because primary teeth require using a resorbable and noncondensable filling material.…”

Molecular screening the emergence of vancomycin and gentamycin resistant enterococcus species in burn' patients in basrah government, Iraq

“…While E. faecalis does not ferment arabinose and therefore retains the blue color (Willinger and Manafi, 1995), not all enterococcus species are able to grow in these selective media, but the most clinically relevant species grow well (Garcia-Solache and Rice 2019). Since traditional phenotypic methods are not sufficient, it recommended to use polymerase chain reaction technique PCR with universal primers for 16S rDNA gene that provides a rapid, accurate and more sensitive for detection the species of Enterococcus (Mustafa et al, 2021). There are numbers of worldwide studies were showed frequency bacterial infected burn patients including study of Naqvi et al, 2014 was observed that more infections of burn wound caused by Staphylococcus aureus (23%) then followed Pseudomonas aeruginosa (21%) and less prevalence of Enterococci (1.2%).…”

“…The treatment of these infections has been clinically challenging because of the increasing resistance to different types of antibiotics including prevalence of multidrug resistance (MDR) enterococci. Resistance genes only does not point to pathogenicity of a bacteria, however combined with the virulence determinant it can cause bacteria to become dangerous (Mustafa et al, 2021). Strong biofilm producer exhibits multidrug resistance in many bacterial species, the ability of enterococci to form biofilms contribute to bacterial virulence in several ways including the resistance to antibiotics (Hashem et al, 2017;Shridhar and Dhanashree 2019).…”

Distribution of multidrug-resistant Enterococcus faecalis and Enterococcus faecium isolated from clinical specimens in the Zainoel Abidin General Hospital, Banda Aceh, Indonesia

“…faecalis and ddlE. faecium has been utilized nowadays to rapidly and pricisey identify both enterococcal species (Mustafa et al 2021). The potential benefits of molecular-based approaches over conventional culture and phenotypic testing include better diagnostic accuracy, higher reliability, knowledge about antibiotic resistance, and reduced time and cost (García-Solache and Rice 2019; Sanderson et al 2019).…”