Molecular detection and phylogenetic assessment of six honeybee viruses in <i>Apis mellifera</i> L. colonies in Bulgaria

Shumkova, Rositsa; Neov, Boyko; Sirakova, Daniela; Georgieva, A. I.; Gadjev, Dimitar; Teofanova, Denitsa; Radoslavov, Georgi; Bouga, Maria; Hristov, Peter

doi:10.7717/peerj.5077

Cited by 12 publications

(11 citation statements)

References 50 publications

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“…Our results corroborate the high rates found in these places. However, differing from data commonly observed in the literature, such Shumkova et al (2018), that detected only one case of BQCV in Bulgaria. In Argentina, only 8% of the tested samples were positive for the virus (Molineri et al 2017).…”

Section: Discussioncontrasting

confidence: 99%

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

et al. 2020

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Bees are fundamental in several aspects, especially in relation to plant biodiversity and pollination. Recently, immense losses are being faced in the number of Brazilian colonies, mainly in southern states of the country, which has a strong beekeeping activity. There are indications that, among the reasons for the losses, pathogens that affect the health of bees may be involved. Among them, the microsporidium Nosema and the black queen cell virus (BQCV) stand out for their prevalence. In this study, 92 colonies of 17 apiaries from southern Brazil were evaluated for infection by Nosema ceranae, Nosema apis and BQCV. Nucleic acid extractions and cDNA synthesis were performed from adult bee samples, followed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and multiplex PCR. Eight BQCV positive samples were subjected to sequencing. The results showed that N. ceranae and BQCV are circulating in the Southern region of the country, which may be the reason for the loss of colonies. N. apis was not found. N. ceranae was found in 57.6% (53/92) of the colonies and BQCV in 32.6% (30/92). Co-infection was found in 25% (23/92) of the colonies studied, a factor that is suggested to be reducing the hosts’ longevity due to the synergistic action of the pathogens. The samples submitted to sequencing indicated similarity of 96.8 to 100% between them, in addition to strong similarity with sequences from Asia, United States, Germany and Peru. This study reports the circulation of N. ceranae and BQCV in apiaries in southern Brazil, in addition to being the first phylogenetic analysis of the Brazilian BQCV sequence.

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Section: Discussioncontrasting

confidence: 99%

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

et al. 2020

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“…The highest prevalence of sacbrood disease is observed during early spring, probably due to cold stress induced by fluctuating temperatures [45]. Although SBV is frequently detected in managed European honeybee colonies [46,47,48,49,50,51], only one of 43 ejaculate samples was tested positive for this virus. Even though infection is lethal for drone and worker brood, infected colonies usually survive, and thus SBV poses only a moderate threat to managed European honeybees [13].…”

Section: Discussionmentioning

confidence: 99%

Multiple Virus Infections in Western Honeybee (Apis mellifera L.) Ejaculate Used for Instrumental Insemination

Prodělalová

Moutelíková

Titěra

2019

Viruses

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Instrumental insemination of Apis mellifera L. queens is a widely employed technique used in honeybee breeding that enables the effective control of mating. However, drone semen represents a potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of 11 honeybee viruses. In 91% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and A. mellifera filamentous virus (AmFV) in 84%, 19%, 14%, 2%, and 67% of samples, respectively. Single infection (30% of samples) as well as multiple infection (61% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed the high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.

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“…So far, DWV has been the most frequently detected bee virus in honey bees (9,21,33,36,37). It is a non-Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies enveloped virus of the Iflaviridae family with a linear genome of approximately 9-11 kb (44).…”

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confidence: 99%

Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies

Çağırgan¹,

Yıldırım²,

Usta³

2020

Medycyna Weterynaryjna

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Viruses are chiefly responsible for colony losses globally, and deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV) are the most common viral infections in honeybee colonies. As Turkey ranks third in the world in terms of bee colonies, honeybees are of great ecological and economic importance. Thus, the aim of this study was to conduct a phylogenetic analysis of DWV, BQCV, and ABPV, all of which were detected in the southern city of Burdur, which is located along the route that migratory bees take and is a dwelling place for them. Phylogenetic trees were constructed for DWV, BQCV, and ABPV partial nucleotide sequences of the RNA helicase region, the structural polypeptide coding region, and the capsid protein region, respectively. Burdur DWV1 and DWV14 isolates were highly conserved, with 99-100% similarity, and they also shared 93-100% similarity with isolates from Europe and Korea. The two Burdur DWVs had 98% similarity. The DWV1 genome was more similar to the other Turkish isolates than the DWV14. Burdur BQCV2 and BQCV19 isolates, which were highly conserved with 97-99% similarity, formed the same cluster with other Turkish isolates but excluding the Turkey Koycegiz isolate. Burdur ABPV isolates were highly conserved with 99% similarity. Although they showed 94-97% similarity with other European isolates, they were in the same cluster as Turkish isolates. Thus, it was found that although the DWV, BQCV, and ABPV isolates obtained as a result of the present study were highly conserved, they showed differences in relation to the DWV, BWCV, and ABPV isolates that were isolated from different geographical regions.

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Molecular detection and phylogenetic assessment of six honeybee viruses in Apis mellifera L. colonies in Bulgaria

Cited by 12 publications

References 50 publications

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

Black queen cell virus and Nosema ceranae coinfection in Africanized honey bees from southern Brazil

Multiple Virus Infections in Western Honeybee (Apis mellifera L.) Ejaculate Used for Instrumental Insemination

Phylogenetic analysis of deformed wing virus, black queen cell virus and acute bee paralysis viruses in Turkish honeybee colonies

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