2019
DOI: 10.3390/v11040306
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Multiple Virus Infections in Western Honeybee (Apis mellifera L.) Ejaculate Used for Instrumental Insemination

Abstract: Instrumental insemination of Apis mellifera L. queens is a widely employed technique used in honeybee breeding that enables the effective control of mating. However, drone semen represents a potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of 11 honeybee viruses. In 91% of samples, viral infection was detected. The survey revea… Show more

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Cited by 21 publications
(20 citation statements)
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“…In the phylogenetic analysis of partial capsid protein gene (Fig.1), the sequences of this study (BR-BQCV) showed greater similarity with Asian sequences and sequences from the United States (USA), Germany and Peru. The sequences from Belgium, Kyrgyzstan and Georgia are grouped into a distinct cluster, suggesting that geographic origins of sequences can be decisive for their genetic divergence (Roberts et al 2017, Milićević et al 2018, Prodělalová et al 2019. The sequence from Brazil D6 (EU292211), collected in 2007 in the Southeast region, was grouped in a different cluster when compared with the samples from this study.…”
Section: Discussionmentioning
confidence: 58%
See 1 more Smart Citation
“…In the phylogenetic analysis of partial capsid protein gene (Fig.1), the sequences of this study (BR-BQCV) showed greater similarity with Asian sequences and sequences from the United States (USA), Germany and Peru. The sequences from Belgium, Kyrgyzstan and Georgia are grouped into a distinct cluster, suggesting that geographic origins of sequences can be decisive for their genetic divergence (Roberts et al 2017, Milićević et al 2018, Prodělalová et al 2019. The sequence from Brazil D6 (EU292211), collected in 2007 in the Southeast region, was grouped in a different cluster when compared with the samples from this study.…”
Section: Discussionmentioning
confidence: 58%
“…The transmission of BQCV occurs mainly through the fecal-oral route. It has been reported the active replication of virus in drones and the transmission through semen (Alger et al 2019, Prodělalová et al 2019. BQCV has already been detected in flowers collected from the apiaries (Alger et al 2019), and in samples of honey (Milićević et al 2018).…”
Section: Introductionmentioning
confidence: 99%
“…Because of the challenges in experimentally controlling the natural mating process of honey bee queens and drones, much of the evidence for venereal infection is based on experiments with artificial insemination (Yue et al, 2006; de Miranda and Fries, 2008) the detection of viral particles in the reproductive organs, tissues and secretions of drones and queens (e.g., endophallus, semen, ovaries, spermatheca). That is the case for the detection of ABPV (Yue et al, 2006;Prodělalová et al, 2019), BQCV (Prodělalová et al, 2019), SBV (Prodělalová et al, 2019), AmFV (Gauthier et al, 2015;Prodělalová et al, 2019), IAPV (Chen et al, 2014), and DWV (Fievet et al, 2006;Yue et al, 2006;de Miranda and Fries, 2008;Yañez et al, 2012a;Prodělalová et al, 2019) in semen, which first identified the potential for sexual transmission. Similarly, the occurrence of viruses in the spermatheca of mated queens such as DWV (Chen et al, 2006;de Miranda and Fries, 2008;Francis et al, 2013) and IAPV (Chen et al, 2014) suggests the potential for virus found in sperm to cause infection in the queen tissues; confirming the possibility for sexual transmission through artificial insemination (Yue et al, 2007;de Miranda and Fries, 2008).…”
Section: Venereal Transmissionmentioning
confidence: 99%
“…Mating seems to be an important transmission route for viruses to infect queens. Multiple viruses, such as DWV, ABPV, BQCV, and SBV, have been detected from collected seminal fluid of apparently healthy drones, providing evidence that queens can be infected by mating or instrumental insemination [71,72]. Drones are favorable hosts for Varroa mites, which could elevate virus titers and the prevalence of viruses in drones.…”
Section: Viral Transmission Modes To Honey Bee Queensmentioning
confidence: 99%