Molecular detection of adulteration in commercial buffalo meat products by multiplex PCR assay

Abstract

Authentication of commercial buffalo meat products has become a market concern. This study intended to develop and validate a highly species-specific multiplex PCR assay for authentication of buffalo meat products. Four pairs of species-specific primers were used to target mitochondrial cytochrome oxidase O (CDO) gene. The assay generated the expected PCR products of 313, 255, 294 and 177 bp for buffalo meat, cattle meat, pork meat and duck meat, respectively. The multiplex PCR assay was sensitive enough to detect 1 pg pure DNA and 0.1% (w/w) adulterated meat under mixed matrices. Market survey revealed about 35.3% of buffalo meat products are adulterated with cattle meat, pork meat or duck meat in China. The adulteration was found in all food product types including minced meat, frozen rolls, boiled meat, meat ball, vacuum-packed meat and jerky. These findings showed that multiplex PCR assay are potentially reliable techniques for detection of adulteration in raw and processed buffalo meat products.

Detection of Pork in Canned Meat Products by using DNA-based Methods

“…However, the tendency to mix pork meat and fat into the processed meats is more frequent in other countries due to high production of pork flesh and its cheapness. The previous study found that meat products that were declared as buffalo contained pork 7,13 . In addition, beef and mutton meat might replace in chicken, horse, and donkey meat.…”

“…However, DNA detection technique is considered as reliable, efficient, simple and a quick method to identify meat species 6 . In previous studies, testing processed of meat products by DNA detection technique revealed the use of a label that is incorrect (7) . Since becoming aware of these issues, this work aimed to identify pork in canned meat products by detection the meat specie in it under laboratory conditions.…”

Evaluation of sterol composition in different formulations of cocoa milk as milk fat purity indicator

“…The effectiveness of NIR spectroscopy for authentication and characterization of fats in cow and buffalo ghee (anhydrous milk fat) has been successfully proved as well (Antony et al, 2018). It is worthy to mention that there are also other promising analytical methods such as polymerase chain reaction (PCR) assay whose accuracy and reliability as authentication tools have been reported for meat product (Wang et al, 2019).…”

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

“…Similar techniques have also been used to assess meat’s adulteration for human consumption (see, for example, [ 113 , 114 ]). Besides, to this day, some reference materials regarding species identification have been successfully produced to test laboratory adeptness (for example, the Dem Deutschen Referenzbüro für Ringversuche & Referenzmaterialien, which organize proficiency testing schemes for the determination in animal species for food matrices such as gelatin and candy).…”