MOLECULAR DETECTION OF ANAPLASMA MARGINALE IN THE EGYPTIAN WATER BUFFALOES (BUBALUS BUBALIS) BASED ON MAJOR SURFACE PROTEIN 1α

Elhariri, Mahmoud; Elhelw, Rehab; Hamza, Dalia; Soliman, Doaa

doi:10.21608/jesp.2017.77758

Cited by 14 publications

(9 citation statements)

References 30 publications

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“…These signs are common, although they are not pathognomonic; animals with persistent infections can be asymptomatic carriers. Carrier animals with no clinical symptoms are thought to be a major reservoir of infection for ticks, which can spread the disease to other animals [ 12 , 18 , 26 ]. The goal of this study was to investigate Piroplasma and Anaplasmataceae DNA in various animal hosts (cattle, sheep, goats, buffaloes, donkeys, and horses) and their associated ticks across six provinces in Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…For anaplasmosis, the overall prevalence rate of anaplasmosis was 18.8% in all animal species, except horses. In Egypt, several studies have recorded the endemicity of anaplasmosis in cattle [ 1 , 4 , 24 , 28 , 38 , 39 ], buffaloes [ 4 , 12 ], sheep [ 4 , 35 ], and donkeys [ 40 ]. However, anaplasmosis ( A. ovis ) was firstly detected in goats in Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…Affected animals remain carriers for the rest of their lives. Anaplasmosis causes significant economic losses in developing countries owing to its endemic nature [ 4 , 12 ]. The main signs of bovine anaplasmosis are quite varied, ranging from subclinical chronic infections to severe instances accompanied by fever, hemolytic anemia, abortions, productivity losses, and mortality [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Anaplasmosis causes significant economic losses in developing countries owing to its endemic nature [ 4 , 12 ]. The main signs of bovine anaplasmosis are quite varied, ranging from subclinical chronic infections to severe instances accompanied by fever, hemolytic anemia, abortions, productivity losses, and mortality [ 12 , 13 ]. Bovine anaplasmosis, which affects various animals, is endemic in tropical and subtropical areas [ 4 , 8 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, carrier and subclinical case detection is a serious step for appraising the level of risk caused by piroplasms ( Theileria and Babesia ) [ 18 ]. Similarly, because anaplasmosis is endemic in Egypt, herd screening for Anaplasmataceae was suggested in the absence of signs or symptoms of infection [ 12 ]. Because of the low bacteremia, detecting infection in carriers by standard microscopy techniques is difficult.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

et al. 2022

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Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. A total of 234 blood samples and 95 adult ticks were collected from animal hosts (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, and 8 horses) from six provinces of Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, and Matruh). Blood and tick samples were investigated by polymerase chain reaction coupled with sequencing targeting 18S and 16S RNA genes for Piroplasma sp. and anaplasmataceae, respectively. Statistical analysis was conducted on the potential epidemiological factors. Of the 234 animals examined, 54 (23.08%) were positive for pathogens DNA distributed among the six provinces, where 10 (4.27%) were positive for Piroplasma sp., 44 (18.80%) for anaplasmataceae, and 5 (2.14%) were co-infected. Co-infections were observed only in cattle as Theileria annulata and Anaplasma marginale plus Babesia bigemina, A. marginale plus B. bigemina, and T. annulata plus B. bigemina. Piroplasmosis was recorded in cattle, with significant differences between their prevalence in their tick infestation factors. Animal species, age, and tick infestation were the potential risk factors for anaplasmosis. All ticks were free from piroplasms, but they revealed high prevalence rates of 72.63% (69/95) with anaplasmataceae. We identified T. annulata, B. bigemina, and A. marginale in cattle; A. platys in buffaloes; A. marginale and A. ovis in sheep; for the first time, A. ovis in goats; and Ehrlichia sp. in Rhipicephalus annulatus ticks. Our findings confirm the significant prevalence of piroplasmosis and anaplasmosis among subclinical and carrier animals in Egypt, highlighting the importance of the government developing policies to improve animal and public health security.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

et al. 2022

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Molecular characterization of some equine vector-borne diseases and associated arthropods in Egypt

et al. 2022

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Prevalence of tick-borne haemoparasites and their perceived co-occurrences with viral outbreaks of FMD and LSD and their associated factors

Abas

Abdelrahman

Saleh

et al. 2021

Heliyon

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Species of Theileria , Babesia , and Anaplasma are Tick-borne pathogens (TBPs) that are prevalent throughout the world, particularly in the tropical and subtropical regions. Associated diseases of Theileriosis, Babesiosis , and Anaplasmosis , respectively, represents a major threat to livestock production in many countries. TBPs have a high prevalence in different geographical locations in Egypt. Foot and mouth disease (FMD) and Lumpy skin disease (LSD) are considered endemic bovine viral diseases in Egypt. Our clinical observations during the epidemics of LSD and FMD viruses showed higher prevalence rates for the TBPs. To investigate this correlation, a total of 670 samples from cattle and buffalo were collected during the summers of 2017 and 2018 distributed throughout ranches and smallholders in two geographical locations in Egypt. Two farms with a recent clinical outbreak of LSD with a total of 270 animals, while the other location included three farms with a recent FMD outbreak with a combined 400 cattle. Examined animals were classified mainly according to age, gender, species, breed (native versus crossbred), and the presence of ticks. Whole blood samples were collected for TBPs and viral (LSD and FMD) examinations, while tissue specimens were collected for detection of FMD and LSD viruses by real-time PCR. Our results confirmed significantly higher prevalence rates for the TBPs in LSD-positive than LSD-negative animals, while no significant difference could be detected for the prevalence rate of the TBPs in the FMD positive and negative groups. The prevalence of Babesia and Theileria was significantly (P < 0.05) higher in cross-breeds than native cattle. Infections with Anaplasma and co-infections with Babesia - Anaplasma and Theileria - Anaplasma were significantly higher in native than cross-breeds cattle. The intensity of parasitic infection (parasitemia) has a significant difference in the positive groups for the two viruses compared to the negative groups. These results collectively confirming the enhancing role of LSD on the prevalence rate of the haemoprotozoal infections leading to more serious outcomes to the livestock infections, and therefore the control of haemoprotozoal infections should be implemented as a part of viral epidemics control.

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MOLECULAR DETECTION OF ANAPLASMA MARGINALE IN THE EGYPTIAN WATER BUFFALOES (BUBALUS BUBALIS) BASED ON MAJOR SURFACE PROTEIN 1α

Cited by 14 publications

References 30 publications

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks

Molecular characterization of some equine vector-borne diseases and associated arthropods in Egypt

Prevalence of tick-borne haemoparasites and their perceived co-occurrences with viral outbreaks of FMD and LSD and their associated factors

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