Molecular detection of Anaplasma ovis in small ruminants and ixodid ticks from Mongolia

Enkhtaivan, Batsaikhan; Narantsatsral, Sandagdorj; Davaasuren, Batdorj; Otgonsuren, Davaajav; Amgalanbaatar, Tovuu; Uuganbayar, Erdenekhuu; Zoljargal, Myagmar; Myagmarsuren, Punsantsogvoo; Suganuma, Keisuke; Molefe, Nthatisi Innocentia; Sivakumar, Thillaiampalam; Inoue, Noboru; Battur, Banzragch; Battsetseg, Badgar; Yokoyama, Naoki

doi:10.1016/j.parint.2018.11.004

Cited by 27 publications

(14 citation statements)

References 27 publications

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“…The average prevalence of A. ovis (11%, 56/509) in goats in the present study was lower than that in France (52%) [ 54 ], Mongolia (71.3%) [ 55 ], and other regions in China, e.g., Guizhou (17.8%), Xinjiang (40.5%), Zhejiang (26.3%) [ 18 , 19 ], but higher than that in Heilongjiang (2.6%), Henan (8.7%), and Hubei (7.2%) in China [ 18 , 21 ]. One previous study reported lower prevalence of A. ovis (0.9%) in goats in Xi’an city, Shaanxi [ 56 ], and the other study found higher prevalence of A. ovis (25.2%) in goats in Weinan city, Shaanxi [ 22 ].…”

Section: Discussioncontrasting

confidence: 66%

Molecular Characterization of Anaplasma spp. among Dairy, Cashmere, and Meat Goats in Shaanxi Province, Northwestern China

Yang

et al. 2022

Animals

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Anaplasma spp. are important tick-borne pathogens endangering the health of humans and various animals. Although several studies have reported Anaplasma infection in livestock in China, little is known about the impact of production categories on the occurrence of Anaplasma species. In the present study, PCR tools targeting the 16S rRNA and msp4 genes were applied to investigate the prevalence of Anaplasma spp. in 509 blood samples of dairy (n = 249), cashmere (n = 139), and meat (n = 121) goats from Shaanxi province. The prevalence of Anaplasma spp. was 58.5% (298/509) in goats, and significant differences (p < 0.001) were identified in the prevalence among production categories, with the highest in meat goats (84.3%, 102/121), followed by cashmere goats (58.3%, 81/139) and dairy goats (46.2%, 115/249). Significant differences (p < 0.001) in prevalence were also found among sampling sites and age groups. Meanwhile, the prevalence was 36.9% (188/509) for A. phagocytophilum, 36.1% (184/509) for A. bovis, and 11.0% (56/509) for A. ovis, and significant differences (p < 0.001) in prevalence of A. phagocytophilum, A. bovis and A. ovis were recognized among production categories and sampling sites. A. phagocytophilum, A. bovis and A. ovis were dominant species in meat, dairy, and cashmere goats, respectively, and A. ovis was absent in meat goats. Co-infections were found in 124 (24.4%) investigated samples. Goats aged < 2, 3–6, and 7–12 months, and goats from Qingjian and Zhenba were risk factors associated with the occurrence of Anaplasma. Phylogenetic analysis indicated separate clades for the distribution of A. phagocytophilum from different ruminant, reflecting potential host adaption within this species. This study reported the colonization occurrence of Anaplasma spp. among production categories in goats in Shaanxi province and enriched our knowledge on the transmission of Anaplasma spp. in goats in China. Considering the existence of zoonotic A. phagocytophilum in goats in this study and previous reports, interventions based on One Health are needed to be developed to control the transmission of Anaplasma spp. between humans and animals.

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Section: Discussioncontrasting

confidence: 66%

Molecular Characterization of Anaplasma spp. among Dairy, Cashmere, and Meat Goats in Shaanxi Province, Northwestern China

Yang

et al. 2022

Animals

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“…This also held true when examining previous exposure to Anaplasma spp., which was detected in 37% of nomadic herders and over 40% of livestock . We also have detected Anaplasma ovis infection rates as high as 80% in sheep and 69% in goats, which aligns with what has previously been detected in ticks from the same region (Ochirkhuu et al, 2017;von Fricken et al, , 2020aEnkhtaivan et al, 2019;Fischer et al, 2020;Chaorattanakawee et al, 2022). Anaplasmosis in livestock can result in anoxia, abortions, infertility, significant weight loss, and even death, all of which can impact economic security in pastoralist communities.…”

Section: Introductionsupporting

confidence: 91%

Metagenomic profiles of Dermacentor tick pathogens from across Mongolia, using next generation sequencing

et al. 2022

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Tick-borne diseases are a major public health concern in Mongolia. Nomadic pastoralists, which make up ~ 26% of Mongolia’s population, are at an increased risk of both tick bite exposure and economic loss associated with clinical disease in herds. This study sought to further characterize tick-borne pathogens present in Dermacentor ticks (n = 1,773) sampled in 2019 from 15 of Mongolia’s 21 aimags (provinces). The ticks were morphologically identified and sorted into 377 pools which were then screened using Next-Generation Sequencing paired with confirmatory PCR and DNA sequence analysis. Rickettsia spp. were detected in 88.33% of pools, while Anaplasma spp. and Bartonella spp. were detected in 3.18 and 0.79% of pools, respectively. Khentii had the highest infection rate for Rickettsia spp. (76.61%; CI: 34.65–94.79%), while Arkhangai had the highest infection rate for Anaplasma spp. (7.79%; CI:4.04–13.72%). The exclusive detection of Anaplasma spp. in tick pools collected from livestock supports previous work in this area that suggests livestock play a significant role in disease maintenance. The detection of Anaplasma, Bartonella, and Rickettsia demonstrates a heightened risk for infection throughout Mongolia, with this study, to our knowledge, documenting the first detection of Bartonella melophagi in ticks collected in Mongolia. Further research deploying NGS methods is needed to characterize tick-borne pathogens in other endemic tick species found in Mongolia, including Hyalomma asiaticum and Ixodes persulcatus.

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“…The prevalence of A. ovis has been reported from various parts of the world. The prevalence of A. ovis was reported to be 70.1% in sheep of Tunisa ( 22 ), 69% in sheep of Mogolia ( 23 ), 54.5% in sheep of Qinghai, China ( 24 ), 34.2% in sheep of central and Western Kenya ( 25 ), 29.7% in sheep of Turkey ( 26 ), 20.8% in sheep of Iran ( 27 ), 10% in sheep of West Iran ( 28 ), 5.7% in sheep of South Western China ( 29 ), and 2.6% in sheep of North East China ( 30 ). The variation in the prevalence of Anaplasma ovis between different studies is due to difference in the geographical and climatic conditions of sampling sites, age, gender, immunity of the host animal, tick density in a specific area, and also depends upon type of farm management technique that was followed during a specific study ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep

et al. 2023

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BackgroundMajority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source of income. Anaplasma ovis is known to infect small ruminants globally and causing significant economic losses to livestock owners, however prevalence of Anaplasma ovis has been least investigated from Pakistan despite having a huge sheep population.MethodsThe present study was conducted from June 2021 till December 2021 to report the PCR based prevalence of Anaplasma ovis in the blood samples of sheep (n = 239) that were collected from District Dera Ghazi Khan in Pakistan.ResultsOut of 239 samples, 30 (12.5%) amplified a 347 bp fragment specific for the msp4 gene of Anaplasma ovis. Represented partial msp4 gene sequences were confirmed by Sanger sequencing and deposited to GenBank (OP620757-59). None of the studied epidemiological factors (age, sex, breed, size of herd, dogs with herd, and composition of herd) showed an association (P > 0.05) with the Anaplasma ovis infection in enrolled sheep. Analysis of the amplified partial mSP4 sequence of Anaplasma ovis revealed that this gene is highly conserved as all three sequences were identical and phylogenetically resembled with the msp4 sequences amplified from small ruminants in China, Kenya, and Germany, Turkey, Portugal, Tunisia and India. In conclusion, for the first time, we are reporting a moderate prevalence of Anaplasma ovis prevalence in Pakistani sheep and this data will help in developing the integrated control policies against this newly reported tick-borne disease that is infecting our sheep breeds.

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Molecular detection of Anaplasma ovis in small ruminants and ixodid ticks from Mongolia

Cited by 27 publications

References 27 publications

Molecular Characterization of Anaplasma spp. among Dairy, Cashmere, and Meat Goats in Shaanxi Province, Northwestern China

Molecular Characterization of Anaplasma spp. among Dairy, Cashmere, and Meat Goats in Shaanxi Province, Northwestern China

Metagenomic profiles of Dermacentor tick pathogens from across Mongolia, using next generation sequencing

Molecular prevalence and associated risk factors of Anaplasma ovis in Pakistani sheep

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