Molecular Detection of Avian Mycoplasmas in Poultry Affected with Respiratory Infections in Haryana (India)

Tomar, Piyush; Singh, Y.; Mahajan, N. K.; Jindal, Naresh; Singh, Mahavir

doi:10.20546/ijcmas.2017.606.254

Cited by 11 publications

(10 citation statements)

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“…SPA test indicated the highest seroprevalence against MG in Dera Ismail Khan (43.33%) and lowest in Mansehra (20.86%). Samples collected from Peshawar showed the highest prevalence (23.33%) against MS and the lowest in Abbottabad (11.88%) as already reported by Tomar et al (2017) who recorded 38.77% seroprevalence for both MG and MS in broiler birds of 6-8 weeks old. Similarly Srinivassan et al (2014) indicated 50% seroprevalence of MG and MS in Tamil Nadu.…”

Section: Discussionsupporting

confidence: 71%

“…Out of 390 samples from open shed system, 55 samples were positive (14.10%) as compared to closed shed with 8.91% (n=23) out of 258 samples. In Haryana (India), Tomar et al (2017) reported low prevalence (2.04%) of both MG & MS in individual samples taken from adult birds and more prevalence in day old chicks (4.22%).…”

Section: Discussionmentioning

confidence: 95%

“…Over all presence of MG and MS in broilers may be due to vertical transmission of these agents from breeder and further transmission may be minimized through strict biosecurity measures or promoting control shed in the areas. Moreover, MG and MS may be routinely performed during the early age of chicks so that proper culling of the infected chicks may be practiced at the farms (Michiels et al, 2016;Tomar et al, 2017)…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalance of Mycoplasma gallisepticum and Mycoplasma synoviae in Commercial Broilers and Backyard Poultry in Five Districts of Khyber Pakhtunkhwa-Pakistan

Rehman¹

2018

PVJ

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Serological survey for Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) was conducted in serum samples of broilers and backyard poultry collected from five major districts of Khyber Pakhtunkhwa. The serum samples were examined by serum plate agglutination (SPA) test using stained MG & MS antigens separately. Out of total (n=648) broiler serum samples MG and MS prevalence was recorded as 35.03 and 16.67%, respectively. The highest prevalence of MG was recorded in Dera Ismail Khan 43.33% (65/150) followed by Tank 40% (60/150), Peshawar 36.67% (33/90), Abbottabad 31.46% (45/143) and Mansehra 20.86% (24/115) while in case of MS the highest prevalence was reported in Peshawar 23.33% (21/90) followed by Dera Ismail Khan 20% (30/150), Mansehra 18.26%

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Seroprevalance of Mycoplasma gallisepticum and Mycoplasma synoviae in Commercial Broilers and Backyard Poultry in Five Districts of Khyber Pakhtunkhwa-Pakistan

Rehman¹

2018

PVJ

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“…MS infection in poultry flocks can be diagnosed either serologically, mainly done by serum plate agglutination (SPA) and hemagglutination inhibition, or through direct microbial isolation and identification that represent the gold standard, but time-consuming due to long incubation and fastidious nature of mycoplasmas [6]. Recently, molecular diagnosis with a polymerase chain reaction (PCR) is commonly used [14-16]. SPA test is quick, inexpensive, and sensitive test used for serological screening for Mycoplasma spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

2019

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Aim: Arthritis is one of the most economic problems facing poultry industry worldwide. The study was done to detect possible causes of arthritis in breeder chicken flock with emphasis on molecular identification of Mycoplasma synoviae (MS). Materials and Methods: This study was carried on chicken from broiler breeder flock of 57 weeks' age in Dakahlia, Egypt, suffered from arthritis with frequently 5-7% decrease in egg production, reduced fertility, and hatchability. Forty blood samples were randomly collected from individual birds in sterile tubes and used for serum separation. Serum samples were tested using serum plate agglutination (SPA) test against colored antigens for Mycoplasma gallisepticum (MG), MS, and Salmonella gallinarum-pullorum (SGP). On the other hand, 24 joint samples were collected. Of those 24 samples, 12 joint samples were subjected to bacteriological examination, while the other 12 were utilized for molecular diagnosis by polymerase chain reaction (PCR) for MS and avian reovirus (ARV). Results: SPA test results revealed the presence of antibodies against MG, MS, and SGP in tested sera in rates of 14/40 (35%), 35/40 (87.5%), and 9/40 (22.5%), respectively. Furthermore, 19 bacterial isolates were recognized from joint samples and identified as five Staphylococcus spp., nine Escherichia coli, three SGP, one Citrobacter, and one Proteus. The identified Staphylococcal isolates were three coagulase-positive staphylococci (two Staphylococcus aureus and one Staphylococcus hyicus) and two coagulase-negative staphylococci (one Staphylococcus epidermidis and one Staphylococcus lentus), while E. coli isolate serotypes were 1 O11, 2 O55, 3 O78, 1 O124, 1 O125, and 1 untyped. PCR proved that 12/12 (100%) samples were positive for MS variable lipoprotein hemagglutinin A (vlhA) gene, while ARV was not diagnosed in any of the examined samples. Four amplified vlhA gene of MS isolates (named MS-2018D1, MS-2018D2, MS-2018D3, and MS-2018D4) was successfully sequenced. Analysis of phylogenetic tree revealed the presence of 100% identity between each two sequenced isolates (isolates MS-2018D1 and MS-2018D4 and also isolates 2018D2 and MS-2018D3). However, the nucleotide similarity between four isolates was 88.6%. On the other hand, our field isolates MS-2018D1, MS-2018D4, MS-2018D2, and MS-2018D3 showed nucleotide identity with vaccine strain MS-H 98.4%, 98.4%, 88.1%, and 88.1%, respectively. Furthermore, the nucleotide similarities with field strains from Argentina ranged between 87.8% and 98.6%. Conclusion: Four field isolates of MS were identified in examined broiler breeder flock. A phylogenetic study of these isolates revealed the variation between isolated MS strains and vaccine strain. Therefore, further studies are required for evaluating the vaccine efficacy against the present field isolates of MS. In addition, application of MS immunization of breeder flocks is necessary for proper control of the disease.

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“…17 Though CRD is a well known problem in the poultry industry there are only few reports showing the prevalence of CDR in India employing molecular methods. A study reported 27% MG prevalence in Haryana state of India 19 while a recent study showed that 36 commercial layer farms out of 64 poultry farms were positive for MG by PCR in Namakkal district, Tamil Nadu, India. 20 Another study in Haryana, India shows 18 out of 51 samples were found positive for MG by PCR.…”

Section: Discussionmentioning

confidence: 99%

Chronic respiratory disease outbreak in an organized native chicken farm

Karthik

Bharathi

Mahaprabhu

et al. 2018

JDVAR

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Indian poultry industry is moving with rapid strides in the global market but disease outbreaks cause a major setback to this huge industry. Chronic respiratory disease (CRD) caused by Mycoplasma gallispeticum (MG) is one among the important poultry disease affecting the growth of the industry. This current communication presents the outbreak of CRD among native chicken breeds in an organized farm of India. Post mortem investigation was carried out in the farm as mortality was reported to be 5% and morbidity was 50%. Samples namely trachea, lung, air sac and infra orbital sinus were collected for identifying the causative agent and were subjected to bacteriological and virological detection tests. Samples were found positive for MG by conventional bacteriological method while other bacteriological and virological agents were found negative. Isolates on further characterization using Mycoplasma specific PCR and MG specific PCR revealed that the isolates belong to MG and proved the involvement of the bacterium in causing the disease. Histopathology of the samples also showed the presence of necrotic material and infiltration of lymphocytes. Based on gross pathology, histopathology, isolation and molecular characterization showed that the disease was CRD caused by MG. Thus this reports warrants need for further insights into the clear picture on the status of CRD among native chicken breeds of India so as to carve out a better prevention measure.

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Molecular Detection of Avian Mycoplasmas in Poultry Affected with Respiratory Infections in Haryana (India)

Cited by 11 publications

References 10 publications

Seroprevalance of Mycoplasma gallisepticum and Mycoplasma synoviae in Commercial Broilers and Backyard Poultry in Five Districts of Khyber Pakhtunkhwa-Pakistan

Seroprevalance of Mycoplasma gallisepticum and Mycoplasma synoviae in Commercial Broilers and Backyard Poultry in Five Districts of Khyber Pakhtunkhwa-Pakistan

Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt

Chronic respiratory disease outbreak in an organized native chicken farm

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