Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Duh, Darja; Slovák, Mirko; Saksida, Ana; Strašek, Katja; Petrovec, Miroslav; Avšič-Županc, Tatjana

doi:10.2478/s11756-006-0035-7

Cited by 22 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We detected 35.6% prevalence of B. c. canis in D. reticulatus. Our findings are in contrast to 1% prevalence found by (Duh et al 2006) in Dermacentor ticks collected in western Slovakia as well as to 4.16% prevalence detected by Rar et al (2005). D. reticulatus is present in England and Wales (Hillyard 1996), but the cases of autochthonous canine babesiosis have never been reported there.…”

Section: Canine Babesiosis In Slovakiacontrasting

confidence: 86%

“…Accessibility and high abundance of competent hosts, high ratio of transovarial transmission, geographical, ecological, and other circumstances can increase the infection rate of the pathogen in the vector ticks. High prevalence of Babesia in ticks in our study suggests a possible relation to increased dog presence in localities where the ticks were flagged in contrast to low prevalence in ticks collected in the forest (Duh et al 2006).…”

Section: Canine Babesiosis In Slovakiamentioning

confidence: 74%

See 1 more Smart Citation

Polymerase chain reaction Confirmation ofBabesia canis canisandAnaplasma phagocytophilumin Dogs Suspected of Babesiosis in Slovakia

Majláthová

Majláth

Víchová

et al. 2011

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Canine babesiosis was considered an imported tick transmitted disease until the first case of autochthonous canine babesiosis in Slovakia was described in 2002. Since then, the number of cases kept increasing every year. The causative agent of babesiosis in dogs is not yet characterized; therefore, the aim of our study was to determine the agent and the rate of infection in the vector tick D. reticulatus in Slovakia. Babesia canis canis was detected in 80 out of 87 blood samples from dogs with clinical manifestations of babesiosis. Six dogs suspected of babesiosis tested positive for presence of Anaplasma phagocytophilum, and one mixed infection of B. c. canis and A. phagocytophilum was detected. B. c. canis was detected in 35.6% questing adults of D. reticulatus. The obtained sequences from blood samples showed 99.7% and from D. reticulatus, 99.4% similarity with the B. c. canis (AY072926) from dogs infected in Croatia. In our study, we characterized the agent of canine babesiosis from blood samples of naturally infected dogs and D. reticulatus, the vector tick. Further, the presence of A. phagocytophilum, bacterium responsible for the canine granulocytic anaplasmosis, was recorded in dogs for the first time in Slovakia.

show abstract

Section: Canine Babesiosis In Slovakiacontrasting

confidence: 86%

Section: Canine Babesiosis In Slovakiamentioning

confidence: 74%

Polymerase chain reaction Confirmation ofBabesia canis canisandAnaplasma phagocytophilumin Dogs Suspected of Babesiosis in Slovakia

Majláthová

Majláth

Víchová

et al. 2011

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

show abstract

“…Babesia canis canis , the most common agent of canine babesiosis in Europe (Uilenberg et al , 1989), was detected in a D. reticulatus tick from the vegetation, a tick frequently found parasitizing dogs in the Basque Country. This babesia was recently found in a similar proportion (0.9%, 1/113) in ticks from Slovakia (Duh et al , 2006), and at a higher prevalence in D. reticulatus (3.5%, 3/87) from Russia (Rar et al , 2005). No ticks were B. canis vogeli ‐positive and its vector, Rhipicephalus sanguineus , is not usually collected from the vegetation in the Basque region (Barandika et al , 2006).…”

Section: Discussionsupporting

confidence: 62%

Distribution and molecular detection of Theileria and Babesia in questing ticks from northern Spain

Garcı́a-Sanmartı́n

Barandika

Juste

et al. 2008

Medical Vet Entomology

View full text Add to dashboard Cite

A total of 562 questing adult ixodid ticks, collected during 2003-05 in 10 recreational mountain areas in northern Spain, were analysed for piroplasm infection. Reverse line blot (RLB) analysis using a panel of probes for 23 piroplasm species identified 16 different piroplasms, with an overall prevalence of 9.3%. Most were Theileria spp.-positive (7.7%), 3.0% were positive for Babesia spp. and 1.4% of ticks harboured both genera. Ixodes ricinus (Linnaeus, 1758), the most abundant tick in the vegetation, ranked third with regard to piroplasm infection prevalence (11.4%) after Rhipicephalus bursa (Canestrini & Fanzago, 1878) (16.0%) and Haemaphysalis punctata (Canestrini & Fanzago, 1878) (13.5%). Infection was detected in 6.2% of Dermacentor reticulatus (Fabricius, 1794) and in 1.1% of Haemaphysalis inermis (Birula, 1895), but was absent from Haemaphysalis concinna (Koch, 1844). Ixodes ricinus carried more piroplasm species (13), followed by H. punctata (10), D. reticulatus (8), R. bursa (3) and H. inermis (1). Although most of the positive ticks harboured a single infection (76.9%), mixed infections with two or three different piroplasm species were also detected (23.1%). The various tick-pathogen associations found are discussed and prevalences of infection in ticks are compared with previous results on piroplasms infecting animals in the same region.

show abstract

“…Interestingly, Babesia spp. DNA was only found in I. ricinus ticks, none of the D. reticulatus , the main vector for B. canis [ 42 ], was infected. The most often detected pathogen in I. hexagonus specimens was A. phagocytophilum.…”

Section: Discussionmentioning

confidence: 99%

Detection of selected pathogens in ticks collected from cats and dogs in the Wrocław Agglomeration, South-West Poland

et al. 2016

View full text Add to dashboard Cite

BackgroundTick-borne infections are no longer confined to rural areas, they are documented with increasing frequency in urban settlements across the world. They are known to cause diseases in humans as well as in their companion animals.MethodsDuring a period of 2 years, from January 2013 until December 2014, ticks were collected from dogs and cats in 18 veterinary clinics in the Wrocław Agglomeration, Poland. In total, 1455 ticks were found on 931 pets: 760 domestic dogs and 171 cats. For molecular examinations 127 I. ricinus ticks (115 females and 12 males) were randomly selected, all collected I. hexagonus (n = 137, 32 females, 98 nymphs, 7 larvae) and all collected D. reticulatus (n = 46, 31 females, 15 males) were taken. Ixodes ricinus and I. hexagonus ticks were tested for Rickettsia spp., Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis and Babesia spp., while D. reticulatus ticks were investigated for Rickettsia spp. and Babesia spp. only.ResultsIn total, 65.4 % I. ricinus ticks were infected with at least one pathogen. Over 50 % of I. ricinus were positive for Rickettsia spp. (R. helvetica and R. monacensis). The infection level with A. phagocytophilum was 21.3 %. DNA of Cand. N. mikurensis was detected in 8.1 % I. ricinus ticks. Interestingly only female ticks were infected. The prevalence of Babesia spp. was confirmed in 9.0 % of I. ricinus involving the species B. microti and B. venatorum. A total of nineteen double, one triple and two quadruple infections were found in I. ricinus ticks only. Almost 11 % of I. hexagonus ticks were positive for at least one of the tested pathogens. Rickettsia spp. infection was found in 2.2 %, while A. phagocytophilum was detected in 8.1 % of I. hexagonus ticks. Only one nymph was positive for Cand. N. mikurensis and none of I. hexagonus ticks harbored a Babesia spp. Over 60 % of D. reticulatus ticks were positive for rickettsial DNA, exclusively belonging to the species R. raoultii.ConclusionThe high tick infestation rates and the prevalence of pathogens found in these ticks demonstrate a serious level of encounter to tick-borne diseases in urban dogs in the Wroclaw area, and provide evidence that dogs and cats themselves may substantially contribute to the circulation of the ticks and pathogens in the urban area.

show abstract

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Cited by 22 publications

References 11 publications

Polymerase chain reaction Confirmation ofBabesia canis canisandAnaplasma phagocytophilumin Dogs Suspected of Babesiosis in Slovakia

Polymerase chain reaction Confirmation ofBabesia canis canisandAnaplasma phagocytophilumin Dogs Suspected of Babesiosis in Slovakia

Distribution and molecular detection of Theileria and Babesia in questing ticks from northern Spain

Detection of selected pathogens in ticks collected from cats and dogs in the Wrocław Agglomeration, South-West Poland

Contact Info

Product

Resources

About