Molecular detection of bacterial DNA in venereal‐associated arthritis

Li, Feng; Bulbul, R.; Schumacher, H. Ralph; Kieber‐Emmons, Thomas; Callegari, Peter E.; Feldt, Joan M. Von; Norden, Daniel K.; Freundh, Bruce; Wang, Bin; Imonitie, Victor; Chang, Cheng; Nachamkin, Irving; Weiner, David B.; Williams, William V.

doi:10.1002/art.1780390612

Cited by 60 publications

(32 citation statements)

References 31 publications

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“…Specimens for PCR were transported on dry ice, and DNA was extracted the following day for testing by sensitive and specific assays, as described previously (10)(11)(12), for subsequent PCR for C trachomatis 16S ribosomal RNA (rRNA), and major outer membrane protein (MOMP) (10,12) and for several other organisms. Chlamydia pneumoniae 16S rRNA and MOMP were assessed using 2 sets of primers as described (13,14).…”

Section: Methodsmentioning

confidence: 99%

“…Borrelia burgdorferi outer surface protein A (OspA) was tested for using published primers for OspA and a genomic sequence (15) and using nested primers as described for OspA and OspB (16). Pan bacterial 16S rRNA was assessed using a conserved sequence present across all bacteria using published primers developed by Li et al (11) and by our group (17). For all assays for all organisms targeted, extreme care was taken to avoid crosscontamination of samples or assay preparations; that is, nucleic acids were prepared for analyses in a laboratory distant from that in which the PCR screening assays were prepared and run; separate sets of pipettors were used for DNA preparation and assay setup.…”

Section: Methodsmentioning

confidence: 99%

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Chlamydia trachomatis nucleic acids can be found in the synovium of some asymptomatic subjects

Schumacher¹,

Arayssi

Crane

et al. 1999

Arthritis & Rheumatism

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Objective. The recent identification of antigens or nucleic acids of infectious agents in the joints of patients with reactive arthritis has raised questions about whether chlamydial or other infectious agent nucleic acids are also present in normal joints. We had the opportunity to study synovium from 30 asymptomatic volunteer subjects by use of polymerase chain reaction (PCR) for attempted identification of Chlamydia and other infectious agents. Methods. All subjects had blind needle synovial biopsies with the Parker-Pearson needle. DNA was extracted and PCR performed using primers for Chla-mydia trachomatis, Chlamydia pneumoniae, Borrelia burg-dorferi, and pan bacterial 16S ribosomal RNA (rRNA). Results. Two subjects were identified with nucleic acid for the 16S rRNA gene of C trachomatis. All other PCR reactions were negative except for the pan bacterial 16S rRNA in the C trachomatis-positive subjects. Both subjects, although symptom free, had some evidence of synovial reaction. Conclusion. C trachomatis appears to occasionally be disseminated to joints without producing overt disease. The identification over the past few years of antigens or nucleic acids from Chlamydia (1-7) and several other organisms (see later) in the joints of patients with reactive arthritis has led to a reevaluation of the pathogenesis and treatment of these patients. Our studies and others have shown that evidence of bacterial components (most often Chlamydia trachomatis) in joints, although most common in reactive arthritis, can also occur in patients with other diagnoses (5,8). Since it is known that many other organisms, such as mycobacteria, can be widely disseminated without producing symptoms, we thought that it was important to examine normal control subjects to begin to get some impressions about whether and how often C trachomatis and other bacteria associated with arthritis might be disseminated to joints without producing symptoms. This report describes the first study of apparently normal subjects, a survey of 30 asymptomatic volunteers who consented to blind needle biopsies of knee syno-vium, for evidence of chlamydial or other infectious agents in these joints. SUBJECTS AND METHODS Thirty normal volunteers gave their informed consent and were entered into a protocol approved by the Institutional Review Board at the clinical center at the National Institute of Arthritis and Musculoskeletal and Skin Diseases to study a number of aspects of the features of normal synovium. All subjects were required to have been totally free of any knee or other joint symptoms at any time and to have no objective signs of arthritis. All were also questioned about any antecedent infections that might have later been associated with arthritis, but subjects were not excluded on this basis. All subjects had normal findings on knee radiographs. Results of physical examinations were normal except for mild, painless, retropatellar crepitus noted in 4 subjects. Subjects were age and sex matched to reflect the composition of an ongoing study our g...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chlamydia trachomatis nucleic acids can be found in the synovium of some asymptomatic subjects

Schumacher¹,

Arayssi

Crane

et al. 1999

Arthritis & Rheumatism

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“…To address this issue, several laboratories have used broad-range PCR systems to search for bacterial DNA in synovial materials; most studies targeted SF, and samples from patients with a range of arthritides were analyzed (e.g., see refs. [16][17][18][19][20][21]. With minor exceptions, these studies indicated that DNA from many bacterial groups could be found in the joints of at least some patients.…”

mentioning

confidence: 96%

Chromosomal DNA from a variety of bacterial species is present in synovial tissue from patients with various forms of arthritis

Gérard¹,

Wang²,

Wang³

et al. 2001

Arthritis & Rheumatism

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“…The characteristics of the gene marker HLA-B27 are being further defined. These genes seem to play a role in influencing immune function [14]. It is anticipated that by understanding the effects of each of these known genes, researchers will make significant progress in discovering a cure for ankylosing spondylitis.…”

Section: Resultsmentioning

confidence: 99%

Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

Sharma

Masood

et al. 2012

Ind J Clin Biochem

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Ankylosing spondylitis is a chronic inflammatory disease that has been linked to the human leukocyte antigen class I allele HLA-B27. More than 90 % of patients with ankylosing spondylitis possess the HLA-B27 allele, but only 1 % of people with HLA-B27 develop the disease. Ankylosing spondylitis predominately affects young males. The present study was planned to find out the involvement of HLA-B27 specific allele in relation to age and sex in symptomatic suspected patients of ankylosing spondylitis using conventional PCR technology. Forty symptomatic patients of ankylosing spondylitis were included in the present study. SSP-PCR technique was used to detect the HLA-B27 specific allele. This study showed (out of 40 symptomatic suspected cases of ankylosing spondylitis only 12 patients were detected positive with HLA-B27 allele, while remaining 28 were negative) that the positivity rate for ankylosing spondylitis with HLA-B27 allele is low. Furthermore, it was observed that the males above 50 years are more prone to develop AS with HLA-B27 specific allele. It could be concluded that the conventional PCR technology is a rapid, sensitive, and confirmatory method for the diagnosis of ankylosing spondylitis.

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Molecular detection of bacterial DNA in venereal‐associated arthritis

Cited by 60 publications

References 31 publications

Chlamydia trachomatis nucleic acids can be found in the synovium of some asymptomatic subjects

Chlamydia trachomatis nucleic acids can be found in the synovium of some asymptomatic subjects

Chromosomal DNA from a variety of bacterial species is present in synovial tissue from patients with various forms of arthritis

Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

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