Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients in wasit province, Iraqin wasit province, Iraq

Abstract

ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment and devices is a significant cause of nosocomial infections. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. 30(36.5%) were female and‎‏‎ 52( 63.5%) were male ‎ patients admitted to the intensive care unit distributed 69 (84.1%) gram-‎negative bacteria .while gram positive 13(15.8%), as well as 20 pure ‎isolates of some places, materials and equipment distributed 12 (75%) ‎Gram negative bacteria .While 8 (25%) it is gram positive bacteria. Results of antibiotic susceptibility test only Trimethoprime was effective choice against Acinetobacter baumannii. Phenotypically survey of carbapenem klebsiella pneumoniae ‎were 9(47%), pseudomonas aeruginosa‏ ‏was 7(35%‎‏ of MBL and Carbapenemase and the result of MBL and Carbapenemase for E. Coli 7(43%) similar ‎results (40%). Whereas, Genotypically the most prevalent gene in E.Coli 1 (10%), the result was for Burkholderia cepacia blaVIM 1(100%), its prevalence was 7 (53%) among bacteria Klebsiella pneumonia for NDM, the result of bacteria Pseudomonas aeruginosa for this gene was 4(33.3%) and Escherichia Coli was 1(10%), its prevalence was (100%) among klebsiella oxytoca for KPC.