Molecular detection of cosaviruses in a patient with acute flaccid paralysis and in sewage samples in Germany

Schneider, Julia; Engler, Matthias; Hofmann, Jörg; Selinka, Hans‐Christoph; Jones, Terry C.; Drosten, Christian; Diedrich, Sabine; Corman, Victor M.; Böttcher, Sindy

doi:10.1016/j.virusres.2020.198285

Cited by 1 publication

(1 citation statement)

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“…Even though the maximum viral load (2.4 × 10 5 copies/mL) in our research was one log lower than previous study performed in Germany (2.5 × 10 6 ), the kind of samples were different. 21 However, detection of HCosV directly in CSF even with one log lower than a stool sample may be argue on important of symptomatically HCosV infection in pediatric patients with neurologic diseases. In this report, there was no seasonality for detection of HCosV which was seen high detection rate in cold season.…”

Section: Discussionmentioning

confidence: 99%

First Human Cosavirus Detection From Cerebrospinal Fluid in Hospitalized Children With Aseptic Meningitis and Encephalitis in Iran

Moghaddam

Ghaderi

Parsania

et al. 2021

Pediatric Infectious Disease Journal

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Objective: Human cosavirus (HCosV) is a newly recognized virus that seems to be partly related to nonpolio flaccid paralysis and acute gastroenteritis in pediatric patients. However, the relationship between HCosV and diseases in humans is unclear. To assess an investigation for the occurrence of HCosV among pediatric patients involved in meningitis and encephalitis, we implemented a real-time quantitative polymerase chain reaction assay for detection and quantification of HCosV in stool specimens. Materials and Methods: In this study, a total of 160 cerebrospinal fluid samples from September 2019 to October 2020 were collected from presenting pediatric patients with meningitis and encephalitis in a Karaj hospital, Iran. After viral RNA extraction, the real-time quantitative polymerase chain reaction was performed to amplify the 5′Un-Translated Region region of the HCosV genome and viral load was analyzed. Results: Of the 160 samples tested, the HCosV genomic RNA was detected in 2/160 (1.25%) of samples. The minimum viral load of HCosV was 3.5 × 103 copies/mL from 4 years male patient. The maximum viral load was determined to be 2.4 × 105 copies/mL in one sample obtained from 3.5 years female patient. Conclusions: This is the first documentation of HCosV detection in cerebrospinal fluid samples that better demonstrates relation of HCosV with neurologic diseases including meningitis and encephalitis. Also, these results indicate that HCosV has been circulating among Iranian pediatric patients.

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Section: Discussionmentioning

confidence: 99%