Molecular detection of Cryptosporidium spp. and Enterocytozoon bieneusi in Longjiang Wagyu cattle in Northeastern China

Xue, Nian-Yu; Liu, Feng; Tao, Wei-Fu; Zhao, Quan; Qiu, Hongming; Yang, Huanming; Chen, Yu; Wei, Xinyu; Wang, Wei; Gao, Duo; Ni, Hong-Bo; Wang, Chun-Ren

doi:10.1016/j.micpath.2020.104526

Cited by 15 publications

(5 citation statements)

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“…So far, more than ten Cryptosporidium species, including C. andersoni, C. bovis, C. parvum, C. ryanae, and C. xiaoi, have been detected in cattle (5,6). However, only two species, C. andersoni and C. parvum, were identified in the present study, which was not in agreement with the species reported previously in native beef cattle such as Qinchuan cattle, Longjiang Wagyu cattle, and Yunling cattle in China (13,14,28,30,33). But the Cryptosporidium species identified in this study is identical to the species reported previously in dairy cattle in Beijing (11).…”

Section: Resultscontrasting

confidence: 99%

“…was lower than that in Henan local beef cattle (26.5%), and Qinchuan cattle (20.2%), but higher than that in Longjiang Wagyu cattle (6.4%), Tibetan yellow cattle (0.3%), and Yunling cattle (0.8%) (13,28,30). The prevalence of E. bieneusi was higher than that in Tibetan yellow cattle (5.5%) and Longjiang Wagyu cattle (7.1%) but was lower than in Qinchuan cattle (20.0%), and Henan local beef cattle (9.4%) (14,24,28,30). Additionally, the prevalence of Cryptosporidium spp.…”

Section: Resultsmentioning

confidence: 69%

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Prevalence of Cryptosporidium spp. and Enterocytozoon bieneusi in beef cattle in the Hebei Province of China

SUN¹,

LIN²,

PAN³

et al. 2023

Medycyna Weterynaryjna

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Cryptosporidium spp. and Enterocytozoon bieneusi (E. bieneusi) are two common opportunistic pathogens that can infect humans and animals worldwide. The available data on the prevalence of these pathogens is insufficient in Hebei Province, China, which is located in the Bohai Sea region. In the present study, 718 fecal specimens of native beef cattle from eight farms in Hebei Province were examined for the presence of Cryptosporidium spp. and E. bieneusi using nested PCR targeting the small subunit (SSU) rRNA gene of Cryptosporidium spp. and the internal transcribed spacer (ITS) of E. bieneusi. The prevalence of Cryptosporidium spp. was 9.2% (66/718) and E. bieneusi was 8.6% (62/718). C. andersoni (n = 56) and C. parvum (n = 10) were identified in this study, and all C. parvum-positive specimens belonged to IIdA20G1 in the gp60 gene. The ITS sequence analysis obtained seven known E. bieneusi genotypes, including J (n = 48), BEB4 (n = 4), CHC8 (n = 3), EbpC (n = 2), I (n = 2), D (n = 2), and BEB6 (n = 1). Genotype D and EbpC belonged to the zoonotic Group 1, while the other genotype belonged to the host-adapted Group 2. This is the first report on the occurrence of E. bieneusi in beef cattle in Hebei Province. In this study, the presence of zoonotic C. parvum and two E. bieneusi genotypes suggest that cattle can be a potential zoonotic source for human or animal infection.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 69%

Prevalence of Cryptosporidium spp. and Enterocytozoon bieneusi in beef cattle in the Hebei Province of China

SUN¹,

LIN²,

PAN³

et al. 2023

Medycyna Weterynaryjna

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show abstract

“…Research showed that gut microbial community is closely related to the development of diarrhea ( 14 ). For instance, early studies indicated that the compositions and structures of gut bacterial and fungal communities changed significantly in many diarrheal mammals ( 25 , 26 ). Additionally, fecal microbiota transplantation was shown to alleviate diarrhea in some exploratory experiments, suggesting important roles of gut microbiota in diarrheal prevention and control ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Dynamic changes of gut fungal community in horse at different health states

Lan

Li²,

Yu³

et al. 2022

Front. Vet. Sci.

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Accumulating studies indicated that gut microbial changes played key roles in the progression of multiple diseases, which seriously threaten the host health. Gut microbial dysbiosis is closely associated with the development of diarrhea, but gut microbial composition and variability in diarrheic horses have not been well characterized. Here, we investigated gut fungal compositions and changes in healthy and diarrheic horses using amplicon sequencing. Results indicated that the alpha and beta diversities of gut fungal community in diarrheal horses changed significantly, accompanied by distinct changes in taxonomic compositions. The types of main fungal phyla (Neocallimastigomycota, Ascomycota, and Basidiomycota) in healthy and diarrheal horses were same but different in relative abundances. However, the species and abundances of dominant fungal genera in diarrheal horses changed significantly compared with healthy horses. Results of Metastats analysis indicated that all differential fungal phyla (Blastocladiomycota, Kickxellomycota, Rozellomycota, Ascomycota, Basidiomycota, Chytridiomycota, Mortierellomycota, Neocallimastigomycota, Glomeromycota, and Olpidiomycota) showed a decreasing trend during diarrhea. Moreover, a total of 175 differential fungal genera were identified for the gut fungal community between healthy and diarrheal horses, where 4 fungal genera increased significantly, 171 bacterial genera decreased dramatically during diarrhea. Among these decreased bacteria, 74 fungal genera even completely disappeared from the intestine. Moreover, this is the first comparative analysis of equine gut fungal community in different health states, which is beneficial to understand the important role of gut fungal community in equine health.

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“…Diarrhea is one of the leading causes of decreased productivity and death in ruminants that has been considered a vital factor impeding animal husbandry development in many countries ( Musso et al, 2011 ; Ambalam et al, 2016 ). Early investigations revealed that diarrhea was present in almost all ruminants and especially epidemic in neonatal goat, sheep, cattle, and yak with immature gastrointestinal tract, which caused approximately half of all ruminant deaths ( Li et al, 2018 ; Bu et al, 2020 ; Xue et al, 2020 ). Previous research has indicated that some intestinal microbes including bacteria and fungi of ruminants alternate between preponderant and weak populations accompanied by diarrheic symptoms ( Yang et al, 2017 ; Wang et al, 2018 ; Wang et al, 2019a ).…”

Section: Introductionmentioning

confidence: 99%

Integrated Bacterial and Fungal Diversity Analysis Reveals the Gut Microbial Alterations in Diarrheic Giraffes

Liu

et al. 2021

Front. Microbiol.

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Gut microbiota has been demonstrated to be associated with multiple gastrointestinal diseases, but information regarding the gut microbial alternations in diarrheic giraffe remains scarce. Here, 16S rDNA and ITS gene amplicon sequencing were conducted to investigate the gut microbial composition and variability in diarrheic giraffes. Results demonstrated that Firmicutes and Proteobacteria were the most dominant phyla in the gut bacterial community, whereas Ascomycota and Basidiomycota were observed to be predominant in the gut fungal community regardless of health status. However, the species and relative abundance of preponderant bacterial and fungal genera in healthy and diarrheic giraffes were different. In contrast to the relatively stabilized gut fungal community, gut bacterial community displayed a significant decrease in the alpha diversity, accompanied by distinct changes in taxonomic compositions. Bacterial taxonomic analysis revealed that the relative abundances of eight phyla and 12 genera obviously increased, whereas the relative abundances of two phyla and eight genera dramatically decreased during diarrhea. Moreover, the relative richness of five fungal genera significantly increased, whereas the relative richness of seven fungal genera significantly declined in diarrheic giraffes. Taken together, this study demonstrated that diarrhea could cause significant alternations in the gut microbial composition of giraffes, and the changes in the gut bacterial community were more significant than those in the gut fungal community. Additionally, investigating the gut microbial characteristics of giraffes in different health states is beneficial to provide a theoretical basis for establishing a prevention and treatment system for diarrhea from the gut microbial perspective.

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Molecular detection of Cryptosporidium spp. and Enterocytozoon bieneusi in Longjiang Wagyu cattle in Northeastern China

Cited by 15 publications

References 50 publications

Prevalence of Cryptosporidium spp. and Enterocytozoon bieneusi in beef cattle in the Hebei Province of China

Prevalence of Cryptosporidium spp. and Enterocytozoon bieneusi in beef cattle in the Hebei Province of China

Dynamic changes of gut fungal community in horse at different health states

Integrated Bacterial and Fungal Diversity Analysis Reveals the Gut Microbial Alterations in Diarrheic Giraffes

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