Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil

Abstract

SUMMARYPurpose: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG. Methods: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers. Results: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined. Conclusion: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population.

CerviCal Cytology, HPv DNa aND mrNa – a ComParative StuDy iN 162 PatieNtS

“…Evidence of the deficiencies of the national CC screening program is illustrated by a study in Southern Brazil with 5,485 women in whom previous cytology was reported by 100% of the women diagnosed with carcinoma, while Pap smear in a previous period of less than 3 years was referred by, respectively, 86.5% and 92.8% of women with abnormal cytology and histology (23) . In the present study, HPV DNA was detected in 100% of LSIL and HSIL cases, similar to that reported by Freitas et al (24) , who detected HPV in 93.3% of LSIL. Possibly, the use of MY/GP+ in PCR nested system has increased the sensitivity, as reflected by the additional 27 samples (in 162) found to be HPV reactive.…”

“…Our data showed an HPV prevalence of 39.5%, similar to the 39.6% prevalence obtained using consensus primers PCR findings in a population from Minas Gerais (24) , Brazil, but higher than observed in a regular screening high-risk population in São Paulo (26) , in whom 13.8% of the subjects were HPV positive by consensus primers PCR. Off course the variation in the prevalence rates, when controlled by the HPV determination method, derives from the characteristics of the population, mainly age and sexual activity.…”

HLA-G polymorphisms in women with squamous intraepithelial lesions harboring human papillomavirus

“…Because HPV-16 and -18 are the most frequent types associated with SIL and invasive cervical lesions in Brazil, [28][29][30] attention was focused on them, using previously described specific primers and conditions. 31 The absence of amplification by HPV-16 or -18 primers but the presence of amplification by the MY09 and MY11 primers did not exclude the presence of other HPV types, which in this study were designated as HPVX.…”

Prevalence of cervical human papillomavirus infection in women with systemic lupus erythematosus

“…In this study, the n-PCR technique was used, which combines two diVerent pairs of consensus primers. The advantage of this last technique is the substantial increase in sensitivity [10,15]. However, we cannot exclude the possible contribution of still undeWned genetic factors in the diVerent populations studied.…”