Molecular Detection of
            <i>Orthohantavirus puumalaense</i>
            in Plasma and Urine Samples from Hospitalized Patients Presenting with a Serologically Confirmed Acute Hantavirus Infection in France

Reynes, Jean-Marc; Schaeffer, Laura; Papadopoulos, Pavlos; Ait-Ahmed, Mohand; Siby-Diakite, Dieyenaba; Ripaux-Lefevre, Maryline M; Buivan, Tan-Phuc; Lechat, S; Vray, Muriel; Galempoix, J.M.

doi:10.1128/jcm.00372-23

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Aside from in blood samples, PUUV RNA has been detected using real-time RT-PCR in excreta of infected patients like urine and saliva. Although urine was found inadequate for routine diagnostics in a recent study by others ( 22 ), a role for saliva still needs to be determined. Interestingly, during an outbreak in northern Sweden, 10 of 14 patients hospitalized with NE who had PUUV RNA-positive blood samples also had their saliva tested positive for PUUV RNA ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands

Geeraedts,

Wevers,

Bosma

et al. 2024

Microbiol Spectr

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Laboratory diagnosis of orthohantavirus infection is primarily based on serology. However, for a confirmed serological diagnosis, evaluation of a follow-up serum sample is essential, which is time consuming and causes delay. Real-time reverse transcription polymerase chain reaction (RT-PCR) tests, if positive, provide an immediate and definitive diagnosis, and accurately identify the causative agent, where the discriminative nature of serology is suboptimal. We re-evaluated sera from orthohantavirus-suspected clinical cases in the Dutch regions of Twente and Achterhoek from July 2014 to April 2016 for the presence of Puumala orthohantavirus (PUUV), Tula orthohantavirus (TULV), and Seoul orthohantavirus (SEOV) RNA. PUUV RNA was detected in 11% of the total number ( n = 85) of sera tested, in 50% of sera positive for anti-PUUV/TULV IgM ( n = 16), and in 1.4% of sera negative or indeterminate for anti-PUUV/TULV IgM ( n = 69). No evidence was found for the presence of TULV or SEOV viral RNA. Based on these findings, we propose two algorithms to implement real-time RT-PCR testing in routine orthohantavirus diagnostics, which optimally provide clinicians with early confirmed diagnoses and could prevent possible further invasive testing and treatment. IMPORTANCE The addition of a real-time reverse transcription polymerase chain reaction test to routine orthohantavirus diagnostics may better aid clinical decision making than the use of standard serology tests alone. Awareness by clinicians and clinical microbiologists of this advantage may ultimately lead to a reduction in over-hospitalization and unnecessary invasive diagnostic procedures.

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Section: Discussionmentioning

confidence: 99%

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands

Geeraedts,

Wevers,

Bosma

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

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Glomerular Injury Is Associated with Severe Courses of Orthohantavirus Infection

Nusshag,

Uhrig,

Gruber

et al. 2024

Pathogens

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Hemorrhagic fever with renal syndrome (HFRS) induced by Eurasian pathogenic orthohantaviruses is characterized by acute kidney injury (AKI) with often massive proteinuria. The mechanisms of the organ-specific manifestation are not completely understood. To analyze the role of glomerular and tubular damage in kidney injury induced by HFRS, we measured specific markers in urine samples of patients with acute Puumala virus (PUUV) infection and determined their correlation with disease severity. Levels of α1-microglobulin (α1-MG) and kidney injury molecule 1 (KIM-1), which is expressed by injured tubular epithelial cells, were measured to detect tubular dysfunction and injury. Immunoglobulin G (IgG) and the podocyte specific protein nephrin served as markers for glomerular injury. All four markers were elevated on admission. Markers of glomerular injury, IgG and nephrin, correlated with markers of disease severity such as length of hospitalization, serum creatinine, and proteinuria. In contrast, tubular injury did not correlate with these severity markers. Our results demonstrate that hantavirus infection induces both glomerular and tubular injury early in the clinical course. However, the glomerular dysfunction and podocyte injury seem to contribute directly to disease severity and to play a more central role in HFRS pathogenicity than direct damage to tubular epithelial cells.

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Molecular Detection of Orthohantavirus puumalaense in Plasma and Urine Samples from Hospitalized Patients Presenting with a Serologically Confirmed Acute Hantavirus Infection in France

Abstract: Molecular detection of Orthohantavirus puumalaense (PUUV) RNA during the course of the disease has been studied in blood of patients in Sweden and Slovenia. The use of urine has been poorly investigated.

Cited by 2 publications

References 28 publications

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands

Glomerular Injury Is Associated with Severe Courses of Orthohantavirus Infection

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