Molecular detection of Rotavirus type A in diarrheic calves of Mid-Euphrates governorates\ Iraq

Abstract

<jats:p> Four hundred fecal samples from diarrheic calves of both sexes (1-60 days old) from four Iraqi Mid-Euphrates governorates (Babylon, Al-Najaf, Karbala and Al-Qadissiyiah) were collected to investigate rotavirus type A infection by using Real-Time Polymerase Chain Reaction technique. The results showed that infection rates were (14%), while the high rate (20%) was found in age of (1-30 days), however non-significant values were related to animal gender. Moreover, a high infection was recorded at January (29.5%) and the rotraviral infection was recorded in four provinces but the higher was in Babylon (23%). The clinical examination of infected calves showed voluminous watery diarrhea, profound weakness and mild depression; the body temperature, respiration and heart rates were decreased significantly and appetite was changeable towards loss or anorectic.</jats:p>

The Frequency of Rotavirus Infection among Children under the age 5 years in Erbil Province, Kurdistan Region, Iraq, a hospital based study

“…In relation to the gender factor, boys (41.1%) were tending to be more effected by rotavirus comparing to girls (33.3%). These boy predominate in the present study was in agreement with the results of some local studies [34][35][36][37][38] and some regional neighboring countries also was in agreement with our result that boys are predominant over girls e in infecting with the rotavirus gastroenteritis [39][40][41][42][43] A correlation between the occurrence of rotavirus diarrhea and boy gender has been shown in various research, in addition to other factors including poor education levels, filthy drinking water, and inadequate sanitation [44].…”

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces

“…The present study compatible with study in Missan 48% (12 of 25 samples) and the lowest was in AlQadissiya 28% (7 of 25 samples) (16). When compared to other studies the percentage of rotaviruspositive specimen of our study also compatible with studies reported by many investigators such as 49.4 % in Iran (33) while in Babylon about (23%) of Rotavirus infection was recorded in calves (34). The vital role of molecular detection in this study compatible with (35) and (36) who used also molecular diagnosis and genetic relationship in virus.…”