Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores

André, Marcos Rogério; Dumler, J. Stephen; Scorpio, Diana G.; Teixeira, Rodrigo Hidalgo Friciello; Allegretti, Silmara Marques; Machado, Rosângela Zacarias

doi:10.1016/j.ttbdis.2012.04.002

Cited by 53 publications

(53 citation statements)

References 45 publications

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“…Melo et al (2011), studying this same dogs, reported greater frequency of seropositive dogs as they become older, probably due to a higher chance of becoming infested with ticks over time. Despite the recent reports of ehrlichial agents other than E. canis in Brazil (MACHADO et al, 2006;SILVEIRA et al, 2012;ANDRÉ et al, 2010ANDRÉ et al, , 2012SACCHI et al, 2012), molecular detection of new genotypic variants in Brazilian wildlife animals has been reported, since those animals play the role of sentinels for vector-borne pathogens because they can act as hosts for both bacteria and arthropod, including wildlife of the Pantanal region (WIDMER et al, 2011). However, the present work failed to detect Ehrlichia species different from E. canis infecting dogs from northern Pantanal, even though we used a high sensitive PCR capable of detecting all known Ehrlichia species .…”

Section: Resultsmentioning

confidence: 97%

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Santos

Melo

Moraes‐Filho

et al. 2013

Rev. Bras. Parasitol. Vet.

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The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.Keywords: Dogs, tick borne disease, Ehrlichia canis, Anaplasmataceae, Poconé, Brazil. ResumoO presente estudo avaliou a presença de DNA de Ehrlichia spp. em 320 cães das áreas urbana e rural do município de Poconé, região do Pantanal de Mato Grosso, pela PCR visando o gene dsb. Os fatores de risco para a infecção em cães também foram avaliados. Quarenta e oito (15%, IC 95%: 11,4-19,5%) cães foram positivos, 25 (15,6%, IC 95%: 10,4-22,2%) da área urbana e 23 (14,37%, 95% CI: 9,3-20,8%) da área rural (P > 0,05). Sequências parciais de DNAs obtidos a partir de produtos da PCR de 18 amostras da área urbana e 16 da área rural foram 100% idênticas a E. canis do Brasil e EUA. Este estudo relata a primeira detecção molecular de E. canis em cães da região norte do Pantanal.

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Section: Resultsmentioning

confidence: 97%

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Santos

Melo

Moraes‐Filho

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…André et al (30) detected positivity of 21% in Ehrlichia spp. in wild captive felids in Brazil, among which 52% contained ehrlichial DNA closely related to E. chaffeensis, a species associated to human monocytic ehrlichiosis (31) .…”

Section: Discussionmentioning

confidence: 97%

SEROLOGICAL EVIDENCE OF EXPOSURE TO Ehrlichia canis IN CATS

et al. 2016

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The aim of the present study was to estimate the occurrence of Ehrlichia canis in cats from the semiarid region of Northeast of Brazil. Sera of 101 healthy cats were submitted by Indirect Immunofluorescence Assay (IFA), and considered positive when antibody titers ≥ 40 were obtained. Seroprevalence of 35.6% (36/101) was found, with the following titers: 40 (15 animals); 160 (6); 320 (1); 640 (3), and 2,560 (11). No statistical differences were observed when comparing county of origin, gender, age, breed, and modus vivendi (pet and stray cats), and no ticks were observed in any of the cats. This study revealed exposure to E. canis in cats of the Semiarid Northeast of Brazil. Keywords: Caatinga; ehrlichiosis; feline; hemoparasite; semiarid. ResumoO objetivo do presente estudo foi estimar a ocorrência de Ehrlichia canis em gatos na região semiárida do Nordeste. Soros de 101 gatos saudáveis foram submetidos à Reação de Imunofluorescência Indireta (RIFI), sendo considerados positivos títulos ≥ 40. Soroprevalência de 35,6% (36/101) foi encontrada, com os seguintes títulos: 40 (15 animais); 160 (6); 320 (1); 640 (3) e 2560 (11). Não foram observadas diferenças estatísticas quanto ao município de origem, gênero, faixa etária, raça e modus vivendi (gatos domiciliados e errantes) e não foram observados carrapatos em qualquer um dos animais testados. Este estudo revelou exposição de gatos a E. canis na região semiárida do nordeste brasileiro.

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“…Wild animals play an important role in carrying vectors that may potentially transmit pathogens (EBANI et al, 2008). Several reports highlighted the participation of wild mammals, such as deer, foxes, rodents, and carnivores in A. phagocytophilum cycles, including their role as hosts of these bacteria (LIZ et al, 2000;FUENTE et al, 2008;VERONESI et al, 2011;ANDRÉ et al, 2012, JAHFARI et al, 2014. Wild and domestic animals may share pathogens, as described by Zhan et al (2010), who found goats, sheep, and wild rodents in the same area infected by the same strain of A. phagocytophilum.…”

Section: Introductionmentioning

confidence: 99%

Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

Mongruel

Benevenute

Ikeda

et al. 2017

Rev. Bras. Parasitol. Vet.

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Wild animals play an important role in carrying vectors that may potentially transmit pathogens. Several reports highlighted the participation of wild animals on the Anaplasma phagocytophilum cycle, including as hosts of the agent. The aim of this study was to report the molecular detection of an agent phylogenetically related to A. phagocytophilum isolated from a wild bird in the Midwest of the state of Paraná, Brazil. Fifteen blood samples were collected from eleven different bird species in the Guarapuava region. One sample collected from a Penelope obscura bird was positive in nested PCR targeting the 16S rRNA gene of Anaplasma spp. The phylogenetic tree based on the Maximum Likelihood analysis showed that the sequence obtained was placed in the same clade with A. phagocytophilum isolated from domestic cats in Brazil. The present study reports the first molecular detection of a phylogenetically related A. phagocytophilum bacterium in a bird from Paraná State.

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Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores

Cited by 53 publications

References 45 publications

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

SEROLOGICAL EVIDENCE OF EXPOSURE TO Ehrlichia canis IN CATS

Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

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