Molecular detection of Vibrio cholerae and Vibrio parahaemolyticus from healthy broilers and backyard chickens for the first time in Bangladesh- A preliminary study

Abstract

Many of the Vibrio spp. are major public health concerns globally. Vibrio cholerae and Vibrio parahaemolyticus are the etiology of pandemic and epidemic diarrhea and foodborne illness, respectively. Poultry has the potential to harbor pathogenic Vibrio spp., which can have a detrimental impact on public health if they are transmitted to humans. We, therefore, screened 54 cloacal swab samples from healthy chickens (broiler=27, backyard= 27) to detect V. cholerae and V. parahaemolyticus. Vibrio spp. were isolated and identified by culturing, biochemical tests, PCR, and antibiogram profiles were determined by disk diffusion method. By PCR, 29.63% (16/54; 95% CI: 19.14-42.83%) samples were positive for Vibrio spp., where backyard chickens had a significantly higher (p< 0.05) occurrence (44.44%; 27.59-62.69%) than broilers (14.82%; 95% CI: 5.92-32.48%). V. parahaemolyticus was found in 22.22% (6/27; 95% CI: 10.61-40.76%) of backyard chicken samples, which was significantly dominant (p< 0.05) than in broilers (0/27, 0%, 95% CI: 0.00-12.46%). In addition, V. cholerae was positive in 7.41% (2/27; 95% CI: 1.32-23.37%) of broiler, and 14.82% (4/27; 95% CI: 5.92-32.48%) of backyard chicken samples. The toxR gene was found in all V. cholerae isolates, suggesting the presence of other virulence genes, whereas no isolates of V. parahaemolyticus contained the tdh gene. Isolated Vibrio spp. had high to moderate resistance to tetracycline, azithromycin, erythromycin, and streptomycin. The occurrence of antibiotic-resistant V. cholerae and V. parahaemolyticus in broiler and backyard chickens is of public health concern because of the possibility of food chain contamination

A Systematic Review on the Occurrence of Antimicrobial‐Resistant Escherichia coli in Poultry and Poultry Environments in Bangladesh between 2010 and 2021

“…The spread of AMR is the most divisive issue in the health of humans, animals, and ecosystems in the twenty-first century [ 14 , 15 ]. The spread of AMR has also emerged as a significant barrier to economic development [ 16 ]. The overuse and misuse of antibiotics play a significant role in the emergence and spread of antibiotic-resistant E. coli , which can be transmitted to humans through food or direct contact with sick animals [ 17 ].…”

Prevalence of Campylobacter spp. on retail fresh chicken carcasses in Hanoi, Vietnam

“…The chicken production chain is a potential harbor of the foodborne pathogen (Lampang et al, 2014;Chotinan, 2015;Zhu et al, 2017;Vidayanti et al, 2020;Wangroongsarb et al, 2021;Akter, 2022). Campylobacter is one of the most common bacterial agents causing gastroenteritis in humans worldwide.…”

Properties and Pathogenicity of the Vibrio Bacilli

“…Vibrio cholerae and Vibrio parahaemolyticus have been demonstrated in poultry (broilers and chickens) [35].…”