Molecular determinants of disease and resistance in interactions of Xanthomonas oryzae pv. oryzae and rice

Shen, Yulong; Ronald, Pamela C.

doi:10.1016/s1286-4579(02)00004-7

Cited by 84 publications

(54 citation statements)

References 48 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BLB is a vascular disease in which X. oryzae pv. oryzae grows and colonizes the xylem vessels, eventually clogging them; several virulence-associated determinants have been found, including exopolysaccharide production, hypersensitive response, and pathogenicity (hrp) genes (7,20,26).…”

mentioning

confidence: 99%

OryR Is a LuxR-Family Protein Involved in Interkingdom Signaling between PathogenicXanthomonas oryzaepv. oryzae and Rice

2009

View full text Add to dashboard Cite

Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight in rice, contains a regulator that is encoded in the genome, designated OryR, which belongs to the N-acyl homoserine lactone (AHL)-dependent quorum-sensing LuxR subfamily of proteins. However, we previously reported that X. oryzae pv. oryzae does not make AHLs and does not possess a LuxI-family AHL synthase and that the OryR protein is solubilized by a compound present in rice. In this study we obtained further evidence that OryR interacts with a rice signal molecule (RSM) and that the OryR concentration increases when rice is infected with X. oryzae pv. oryzae. We also describe three OryR target promoters which are regulated differently: (i) the neighboring proline iminopeptidase (pip) virulence gene, which is positively regulated by OryR in the presence of the RSM; (ii) the oryR promoter, which is negatively autoregulated independent of the RSM; and (iii) the 1,4-␤-cellobiosidase cbsA gene, which is positively regulated by OryR independent of the RSM. We also found that the RSM for OryR is small, is not related to AHLs, and is not able to activate the broad-range AHL biosensor Agrobacterium tumefaciens NT1(pZLQR). Furthermore, OryR does not regulate production of the quorum-sensing diffusible signal factor present in the genus Xanthomonas. Therefore, OryR has unique features and is an important regulator involved in interkingdom communication between the host and the pathogen.The species Xanthomonas oryzae includes two pathovars, X. oryzae pv. oryzae and X. oryzae pv. oryzicola, which are pathogens of rice, are closely related, and were initially considered pathovars of Xanthomonas campestris (28). X. oryzae pv. oryzae is a gram-negative rod-shaped bacterium that causes bacterial leaf blight (BLB), one of the most important diseases of rice.

show abstract

mentioning

confidence: 99%

OryR Is a LuxR-Family Protein Involved in Interkingdom Signaling between PathogenicXanthomonas oryzaepv. oryzae and Rice

2009

View full text Add to dashboard Cite

show abstract

“…In phytobacteriology, host range is an important aspect of pathogenicity. Control of diseases can be achieved with resistance genes which reduce host range (50,64). Epidemiological characteristics are highly dependent on host range, and the emergence of new diseases is sometimes correlated with broadened host ranges (74).…”

mentioning

confidence: 99%

From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

Ngoc

Vernière

Jarne

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

Asiatic citrus canker is a major disease worldwide, and its causal agent, Xanthomonas citri pv. citri, is listed as a quarantine organism in many countries. Analysis of the molecular epidemiology of this bacterium is hindered by a lack of molecular typing techniques suitable for surveillance and outbreak investigation. We report a comparative evaluation of three typing techniques, amplified fragment length polymorphism (AFLP) analysis, insertion sequence ligation-mediated PCR (IS-LM-PCR) typing, and multilocus variable-number tandem-repeat analysis (MLVA), with 234 strains originating from Asia, the likely center of origin of the pathogen, and reference strains of pathotypes A, A*, and A w , which differ in host range. The typing techniques were congruent in describing the diversity of this strain collection, suggesting that the evolution pattern of the bacterium may be clonal. Based on a hierarchical analysis of molecular variance, the AFLP method best described the genetic variation found among pathotypes whereas MLVA best described the variation found among individual strains from the same countries or groups of neighboring countries. IS-LM-PCR data suggested that the transposition of insertion sequences in the genome of X. citri pv. citri occurs rarely enough not to disturb the phylogenetic signal. This technique may be useful for the global surveillance of nonepidemiologically related strains. Although pathological characteristics of strains could be most often predicted from genotyping data, we report the occurrence in the Indian peninsula of strains genetically related to pathotype A* strains but with a host range similar to that of pathotype A, which makes the classification of this bacterium even more complicated.

show abstract

“…Bacterial bright is a vascular disease in which X. oryzae pv. oryzae grows and colonizes the xylem vessels, eventually clogging them; several virulence-associated determinants have been found, including exopolysaccharide production, hypersensitive response and pathogenicity (hrp) genes (Cho et al, 2008;Shen & Ronald, 2002). Although bacterial blight is the most important disease of rice from an economic point of view, there are currently no effective antibacterial agents against X. oryzae pv.…”

Section: Introductionmentioning

confidence: 99%

Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit fromXanthomonas oryzaepv.oryzae

et al. 2009

View full text Add to dashboard Cite

Topoisomerase IV is involved in topological changes in the bacterial genome using the free energy from ATP hydrolysis. Its functions are the decatenation of daughter chromosomes following replication by DNA relaxation and doublestrand DNA breakage. In this study, the N-terminal fragment of the topoisomerase IV ParE subunit from Xanthomonas oryzae pv. oryzae was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.15 Å resolution using a synchrotron-radiation source. The crystal belonged to space group P4 2 2 1 2, with unit-cell parameters a = b = 105.30, c = 133.76 Å . The asymmetric unit contains one molecule, with a corresponding V M of 4.21 Å 3 Da À1 and a solvent content of 69.6%.

show abstract

Molecular determinants of disease and resistance in interactions of Xanthomonas oryzae pv. oryzae and rice

Abstract: Xanthomonas oryzae pv. oryzae is the causal agent of rice bacterial blight disease. Numerous genes critical for virulence have been identified. This article reviews current knowledge on the molecular mechanisms of X. oryzae pv. oryzae virulence.

Cited by 84 publications

References 48 publications

OryR Is a LuxR-Family Protein Involved in Interkingdom Signaling between PathogenicXanthomonas oryzaepv. oryzae and Rice

OryR Is a LuxR-Family Protein Involved in Interkingdom Signaling between PathogenicXanthomonas oryzaepv. oryzae and Rice

From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit fromXanthomonas oryzaepv.oryzae

Contact Info

Product

Resources

About