Molecular determination of antimicrobial resistance in Escherichia coli isolated from raw meat in Addis Ababa and Bishoftu, Ethiopia

Messele, Yohannes Equar; Abdi, Reta D.; Yalew, Shimels Tikuye; Tegegne, Desiye Tesfaye; Emeru, Bezina Arega; Werid, Gebremeskel Mamu

doi:10.1186/s12941-017-0233-x

Cited by 49 publications

(46 citation statements)

References 31 publications

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“…Besides, low doses of tetracycline have been used to control weaning diarrhoea and also included in the feed as antibiotic feed additives (AFAs) for growth promotion [5]. The results observed in this study are in agreement with studies done in Ethiopia, Iran, and Pakistan [41–44]. Meanwhile, our findings contradict findings of earlier studies done in South Africa and Poland that reported lower prevalences [25,29].…”

Section: Discussionsupporting

confidence: 87%

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Jaja

Oguttu

Green

et al. 2019

Preprint

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28Objective: This study aimed to characterise antibiotics resistance of Escherichia coli isolates 29 from the formal meat sector (FMS) and informal meat sectors (INMS). 30Method: A total of 162 and 102 E. coli isolates from the FMS, and INMS respectively were 31 isolated by standard culture-based, and biochemical reactions. The isolates were further 32 confirmed by polymerase chain reaction (PCR). The disc diffusion method was used to 33 screen for antimicrobial susceptibility against 19 different antibiotics. The presence of class 34 1-2 integrons in each E. coli isolates was assessed using 3-CS and 5-CS regions specific 35 primers. 36Result: Among the 19 antimicrobials, resistance to tetracyclines, aminoglycosides, 37 cephalosporins, and nitrofurans were found to be more frequent than carbapenems and 38 phenicol with a noticeable increase in the number of multi-drug resistance ranging from three 39 to ten antimicrobials. A total of 20 resistance determinants were assessed with their 40 prevalence and distributions obtained as follows for FMS and INMS respectively; 41 [aminoglycosides: aadA (40.6%; 31.9%), aacC2 (21.4%; 31%), aphA1 (20.8%; 15.1%), 42 aphA2 (37.7%; 18.9%) and strA (6.5%; 9.4%)], [β-lactams: ampC (20%; 45%), blaTEM, 43 (4.4%; 13.3), and blaZ (8.9%; 2.2%)], [Chloramphenicol: catI (1.7%; 1.7%), and cmIA1 44 (1.7%; 1.7%)] and [tetracyclines: tetA (7.7%; 15.4%), tetB (11.5%; 24%), and tetM, (1.9%; 45 8.7%)], and [sulfonamides: sul1 (22.2%; 26.7%), sul2 (17.8%; 6.7%)]. 46 Conclusion: Multiple antibiotic resistance (MAR) indexes ranged from 0.2 -0.5. The results 47 reveal a high prevalence of multidrug-resistant E. coli isolates and resistance determinants 48 suggesting that consumers and handlers of such meat are at risk of contracting antibiotic 49 resistant E. coli-related foodborne disease. 50 51 1 Background 53Meat is a high-risk food, and many studies have shown a causal relationship between meat 54 consumption and disease outbreaks. Meat-related foodborne diseases frequently occur due to 55 the consumption of Escherichia coli contaminated raw or poorly processed meat. The United 56 States, Center for Disease Control and Prevention (CDC) estimates for the year 2011 that one 57 in six or forty-eight million people are infected with a foodborne illness each year, resulting 58 in 3000 deaths. Of this number, E. coli O157: H7 caused an estimated 73480 illnesses each 59 year, resulting in more than 2000 hospitalisations and 60 deaths [1]. 60 Even though raw food hygiene and food safety epidemiology is still in its infancy in Africa, 61 foodborne disease (FBD) is common in developing countries. For instance, 10200 cases of 62 Shiga toxin E.coli (STEC) related FBD was reported in WHO subregion AFR D and E circa 3 63 2012 [2]. The actual prevalence of food-borne infections is difficult to determine, primarily 64 because only a small percentage of incidence is officially reported. Even when cases of 65 foodborne infections are reported, only in a limited number is the aetiology determined [3]. 6...

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Section: Discussionsupporting

confidence: 87%

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Jaja

Oguttu

Green

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…According to the World Organization for Animal Health (OIE, 2018), penicillin G, tetracycline, and amoxicillin-clavulanic acid are "critically important antimicrobial agents," while cefazolin and cefalotin are categorized as "highly important antimicrobial agents" in veterinary medicine (OIE, 2018). High levels of resistance to such antimicrobials have also been reported by other authors in bacteria isolated from beef (Cameron & McAllister, 2016;EFSA & ECDC, 2019;Hiroi et al, 2012;Jaja, Bhembe, Green, Oguttu, & Muchenje, 2019;Messele et al, 2017). The high prevalence of resistance or reduced susceptibility to antibiotics noted in the present work is, nonetheless, surprising…”

Section: Antibiotic Resistance In E Coli Isolates From Beef Preparsupporting

confidence: 86%

Microbial load and antibiotic resistance in raw beef preparations from northwest Spain

González-Gutiérrez

García‐Fernández

Alonso‐Calleja

et al. 2019

Food Science & Nutrition

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Beef preparations (meatballs, minced meat, hamburgers, white sausages, and red sausages) from northwest Spain were tested. Microbial counts ranged from 0.70 ± 0.00 log10 cfu/g (enterococci) to 9.57 ± 0.37 log10 cfu/g (psychrotrophs). In 73.3% of cases, total aerobic counts were higher than the microbiological limits set for the end of the manufacturing process in the European Union (EU Regulation 2073/2005). Forty Escherichia coli isolates were tested against thirteen clinically important antibiotics (disk diffusion method; CLSI). Three of the strains (7.5%) were susceptible to all the antibiotics, four (10.0%) showed resistance or reduced susceptibility to one antibiotic, and 33 (82.5%) were multiresistant (with resistance or reduced susceptibility to between two and eight antibiotics), with an average of 1.85 resistances per strain. The highest rates of resistance were observed for two antimicrobials widely used on cattle farms (cefalotin and tetracycline). The findings in this research emphasize the need to correctly handle beef preparations with the aim of reducing risks to consumers.

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“…Boost incidence of resistance toward chloramphenicol is mostly owing to an excessive application of this forbidden antibioticin poultry fields. Boost incidence of resistance toward chloramphenicol was also conveyed in investigations conducted on the United States [27], Ethiopia [28] and Iran [29]. Similar phenotypic profiles of antibiotic resistance were also conveyed from South Africa [30] (boost incidence of ampicillin and tetracycline), India [31] (boost incidence of resistance toward gentamicin, cephalothin, erythromycin, kanamycin andamikacin), Mexico [32] (boost incidence of resistance toward ampicillin, chloramphenicol, trimethoprimsulfamethoxazole and cephalothin) and South Korea [33] (boost incidence of resistance toward streptomycin, ampicillin, amikacin and tetracycline).…”

Section: Discussionmentioning

confidence: 99%

Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

2020

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E scherichia coli (E. coli) O157 issubstantial foodborne pathogen. The existing survey was addressed to assess the incidence, phenotypic resistance profile and incidence of virulence markers amongst the E. coli O157 isolates recovered from poultry meat. Five-hundred poultry meat samples were collected from Isfahan, Iran.Poultrymeatsamples were cultured and phenotypic antibiotic resistance pattern was deliberated using disk diffusion. PCR was applied to identify the incidence of virulence markers. Forty-four out of 500 (8.80%) poultry meat samples were contaminated with E. coli O157. Duck meat (16%) harbored the uppermost incidence of E. coli O157. Incidence of ehlyA, stx2, stx1and eaeA were 100%, 27.27%, 100% and 100%, respectively. E. coli O157 bacteria exhibited the maximum incidence of resistance against ampicillin (95.45%), tetracycline (88.63%), gentamycin (84.09%)and trimethoprim (38.63%) antibiotics. Incidence of resistance toward imipenem and chloramphenicol were 6.81% and 27.27%, respectively. Poultry meat samples, particularly duck, were considered as reservoir of virulence and antibiotic resistant O157 bacteria. Thoughtful antibiotic prescription and courtesies to the ideologies of food security can condense the hazard of resistant and virulent E. coli O157 in poultry meat.

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Molecular determination of antimicrobial resistance in Escherichia coli isolated from raw meat in Addis Ababa and Bishoftu, Ethiopia

Cited by 49 publications

References 31 publications

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Prevalence and distribution of antimicrobial resistance determinants ofEscherichia coliisolates obtained from meat in South Africa

Microbial load and antibiotic resistance in raw beef preparations from northwest Spain

Incidence and Profiles of Antibiotic Resistance and Virulence Markers of The Escherichia coli O157 Bacteria Recovered From Poultry Meat

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