Molecular diagnosis of COVID-19 in different biologic matrix, their diagnostic validity and clinical relevance: A systematic review

Mahendiratta, Saniya; Batra, Gitika; Sarma, Phulen; Kumar, Harish; Bansal, Seema; Kumar, Subodh; Prakash, Ajay; Sehgal, Rakesh; Medhi, Bikash

doi:10.1016/j.lfs.2020.118207

Cited by 17 publications

(10 citation statements)

References 74 publications

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“…Dust et al [ 16 ] reported the performance of different commercial SARS-CoV-2 RT-PCR assays testing clinical samples and reference material, ranging the sensitivity from 24 copies/mL to 574/mL specimen. However, the RT-PCR sensitivity, specificity, and positive or negative predictive values are still very difficult to determine without clear gold standard tests for SARS-COV-2 [ 17 ]. Previous studies have described positive RT-PCR in asymptomatic pregnant women rates ranging between 50% and 89% [ 8 , 9 , 11 , 12 ], our 66.7 % in our small sample seems to fit well within reported ranges.…”

Section: Discussionmentioning

confidence: 99%

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery

Savirón-Cornudella¹,

Villalba²,

Zapardiel³

et al. 2021

European Journal of Obstetrics & Gynecology and Reproductiv

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To screen pregnant women at risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during delivery using reverse-transcription polymerase chain reaction (RT-PCR) test and serum immunoglobulin (Ig) testing. Method: Between March 31 st and August 31 st of 2020, consecutive pregnant women admitted for labor and delivery in a single hospital were screened for SARS-CoV-2 with nasopharyngeal RT-PCR swab tests and detection of serum IgG and IgM. Results: We studied 266 pregnant women admitted for labor and delivery. The prevalence of acute or past SARS-CoV-2 infection was 9.0 %, including (i) two cases with respiratory symptoms of SARS-Co-V-2 infection and positive RT-PCR; (ii) four asymptomatic women with positive RT-PCR without clinical symptoms and negative serological tests between two and 15 weeks later; and (iii) two women with false positive RT-PCR due to technical problems. All newborns of the 6 pregnant women with RT-PCR positive had negative RT-PCR and did not require Neonatal Intensive Care Unit admission. There were eighteen asymptomatic women with positive serological IgG tests and negative RT-PCR. Conclusion: In our cohort of gravids, we found 2.2 % of women with positive RT-PRC tests and 6.7 % with positive serological tests during the first wave of the SARS-CoV-2 pandemic.

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Section: Discussionmentioning

confidence: 99%

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery

Savirón-Cornudella¹,

Villalba²,

Zapardiel³

et al. 2021

European Journal of Obstetrics & Gynecology and Reproductiv

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“…RT-PCR-based molecular methods represent the gold standard techniques used worldwide to make a confirmatory diagnosis of COVID-19 infection ( 46 ). Since the complete sequencing of the SARS-CoV-2 genome ( 26 ), researchers of different countries have begun to design molecular primers and probes specific to SARS-CoV-2 RNA sequences in order to perform differential diagnosis between COVID-19 infections and other pathologies with similar symptoms, such as seasonal flu or bacterial infections ( 47 , 48 ).…”

Section: Rt-pcr-based Molecular Testsmentioning

confidence: 99%

Current and innovative methods for the diagnosis of COVID‑19 infection (Review)

et al. 2021

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The Coronavirus Disease 2019 (COVID-19) pandemic has forced the scientific community to rapidly develop highly reliable diagnostic methods in order to effectively and accurately diagnose this pathology, thus limiting the spread of infection. Although the structural and molecular characteristics of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were initially unknown, various diagnostic strategies useful for making a correct diagnosis of COVID-19 have been rapidly developed by private research laboratories and biomedical companies. At present, rapid antigen or antibody tests, immunoenzymatic serological tests and molecular tests based on RT-PCR are the most widely used and validated techniques worldwide. Apart from these conventional methods, other techniques, including isothermal nucleic acid amplification techniques, clusters of regularly inter-spaced short palindromic repeats/Cas (CRISPR/Cas)-based approaches or digital PCR methods are currently used in research contexts or are awaiting approval for diagnostic use by competent authorities. In order to provide guidance for the correct use of COVID-19 diagnostic tests, the present review describes the diagnostic strategies available which may be used for the diagnosis of COVID-19 infection in both clinical and research settings. In particular, the technical and instrumental characteristics of the diagnostic methods used are described herein. In addition, updated and detailed information about the type of sample, the modality and the timing of use of specific tests are also discussed.

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“…Metoda detekcije, koja predstavlja zlatni standard za dijagnozu SARS-KoV-2 virusa je RT-PCR test iz nazofaringealnog brisa [42]. Do lažno negativnog nalaza RT-PCR testiranja na SARS-KoV-2 može doći zbog nedovoljne količine virusa, kada se uzorak prerano ili prekasno uzme u toku bolesti, kao i zbog tehničkih grešaka prilikom rukovanja ili slanja materijala.…”

Section: Clinical Presentationunclassified

COOVID-19: What have we learned since the beginning of the epidemic until today?

Milošević¹,

Vujović²

2021

Srpski medicinski časopis Lekarske komore

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In late December 2019, in the city of Wuhan, in China, the appearance of unknown viral pneumonia was recorded in a large number of patients. The cause of this infection was soon discovered - a new coronavirus, called SARS-CoV-2, due to its genetic similarity to the virus that causes severe acute respiratory syndrome (SARS-CoV). The infection then spread rapidly to other continents, and the pandemic is still ongoing. The clinical presentation varies from the asymptomatic form to symptoms of upper respiratory tract infection, and finally to pneumonia and acute respiratory distress syndrome (ARDS). The elderly, immunocompromised patients, and patients suffering from chronic, internal medicine diseases are at risk of the severe form of the COVID-19 disease. The virus enters cells via angiotensin converting enzyme 2 (ACE2) receptors, which are present in practically all tissues in the body. In addition to interstitial pneumonia, pathological changes are also found in other organ systems. The first case in Serbia was recorded on March 6, 2020. A large number of patients required the engagement of health workers of all profiles as well as the introduction of a large number of health institutions into the COVID system. The emergence of a new virus necessitated a new antiviral drug. Based on previous experience with the SARS-CoV virus, previously known antiviral drugs have been used, with varying degrees of success. The therapy changed in accordance with new knowledge, and since the beginning of the epidemic in Serbia, the National Protocol of the Republic of Serbia for the Treatment of the COVID-19 Infection has been established, which has kept apace with the recommendations of the world's leading institutions. The most significant event during the pandemic was the development of the vaccine against COVID-19, with vaccination in Serbia beginning in December 2020. How quickly the epidemic will end depends directly on the speed and efficiency of vaccination, along with other epidemiological measures.

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Molecular diagnosis of COVID-19 in different biologic matrix, their diagnostic validity and clinical relevance: A systematic review

Cited by 17 publications

References 74 publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) universal screening in gravids during labor and delivery

Current and innovative methods for the diagnosis of COVID‑19 infection (Review)

COOVID-19: What have we learned since the beginning of the epidemic until today?

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