Molecular diagnosis of Eimeria stiedae in hepatic tissue of experimentally infected rabbits

Hassan, Khaled; Arafa, Waleed M.; Mousa, Waheed M.; Shokier, Khaled A. M.; Shany, Salama A. S.; Aboelhadid, Shawky M.

doi:10.1016/j.exppara.2016.07.001

Cited by 17 publications

(11 citation statements)

References 22 publications

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“…E. stiedae oocysts were found in rabbit feces 21–28 days after infection. Hassan et al [ 44 ] discovered E. stiedae in feces for the first time at 18 days PI, and the maximum OPG in feces was found between 17 and 21 days PI [ 31 ]. The reduction in the oocyst yield and the disappearance of fecal oocysts in the treated rabbits in this study may have been due to the anticoccidial effects of the AgNPs biosynthesized with L. chinensis , which may have reduced the growth and development of E. stiedae stages, resulting in a reduction in oocyst formation and fecal shedding.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae

Metwally

Alkhuriji

Barakat

et al. 2022

Animals

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The present study used Litchi chinensis peel extract to synthesize silver nanoparticles (AgNPs). This technique is eco-friendly and can be performed in a single step; thus, it has attracted great attention for NP biosynthesis. Herein, we biosynthesized AgNPs with L. chinensis peel extract and examined their anticoccidial activity in rabbit hepatic coccidiosis induced by E. stiedae infection. Thirty-five rabbits were allocated into seven groups: a healthy group (G1), an infected control group (G2), four groups infected before treatment with 10 mg/kg L. chinensis peel extract-biosynthesized AgNPs (G3, G5) or 50 mg/kg amprolium (G4, G6), and rabbits infected after two weeks of pretreatment with 10 mg/kg L. chinensis eel extract-biosynthesized AgNPs (G7). In this study, both pre-and post-treatment with AgNPs produced a substantial reduction in fecal oocyst output, liver enzyme levels, and histopathological hepatic lesions relative to the infected group. In conclusion, L. chinensis peel extract-prepared AgNPs should be considered harmless and efficient in the cure of hepatic coccidiosis in rabbits.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae

Metwally

Alkhuriji

Barakat

et al. 2022

Animals

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“…To date, a total number of 15 valid species have been associated with rabbit eimeriosis; however, 14 are parasites of the intestines, whereas only one species (Eimeria stiedae) infects bile duct epithelial cells and cause very severe and massive damages (4,5). Still, mixed infections are the most common findings, usually obtained during a routine fecal examination, although a molecular diagnosis of hepatic coccidiosis has been recently reported (6).…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Pathomorphological Investigations on Rabbits with Experimentally Induced Hepatic Eimeriosis

Petrova

Iliev

Ivanov

et al. 2022

Macedonian Veterinary Review

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The present study aimed to evaluate the changes in concentrations of some biochemical parameters, as well as macro and microscopic alterations during Eimeria stiedae infection in rabbits. The experiment was performed using 12 three-month-old healthy rabbits, randomly allocated into 2 equal groups: G1 (controls, uninfected animals) and G2 (rabbits infected with E. stiedae). Blood samples were collected at time zero (prior to the infection), 6th, 24th, and 48th hours, and also 7th, 14th, 21st, 28th days after the infection. After sampling, the blood was centrifuged, plasma was separated and frozen at −20 ºC until analyzed. Thawed plasma was used for the quantitative determination of haptoglobin (Hp), total protein (TP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), total cholesterol (TC), total bilirubin (TBIL), urea, and creatinine (CREA). The results in infected rabbits revealed a significant increase in Hp, AST, ALT, GGT, TBIL, and TC levels, as well as a significant decrease in ALP and urea. A weak hyperproteinemia was also observed. There were no changes in CREA concentration. At the end of the clinical investigation, all rabbits were humanely euthanized and necropsied. The post-mortem examination of the infected group revealed hepatomegaly, multifocal yellowish nodules diffusely spread over the liver surface and in the parenchyma, considerably dilated bile ducts, and biliary hyperplasia. Given the results obtained from this experiment, it can be affirmed that hepatic eimeriosis in rabbits is a severe parasitic disease leading to significant disturbances of liver histology and resulting changes in the biochemical profile of infected rabbits.

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“…Rabbit coccidiosis is caused by thirteen species of the genus Eimeria [3]. There are two types of rabbit coccidiosis intestinal ( Eimeria perforans , E. magna , E. media , and E. irresidua ) and hepatic ( E. steidae ) [4,5,6,7]. Intestinal coccidiosis in rabbits is associated with diarrhea, dehydration, inappetence, and weight loss [3,8].…”

Section: Introductionmentioning

confidence: 99%

Prophylactic and Therapeutic Efficacy of Prebiotic Supplementation against Intestinal Coccidiosis in Rabbits

El‐Ashram

Aboelhadid

Abdel-Kafy

et al. 2019

Animals

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Simple SummaryThere are limitations for using chemical products in meat production. The use of prebiotics to control Eimeria infections in rabbits may be of value. Prebiotics as a prophylaxis resulted in diminishing adverse effects caused by Eimeria spp. through decreasing fecal oocyst counts, retaining body weight, and reducing the number of parasitic stages in the intestinal tissues when compared with the non-supplemented controls. AbstractThis study was conducted to investigate the effect of prebiotic supplementation against intestinal coccidiosis in rabbits. Fifty male rabbits aged 35–60 days (1–1.5 kg) were divided into prophylactic and therapeutic experiments (five groups, 10 rabbits per group). Prophylactic experiment had prebiotic supplemented (PS-P), non-supplemented infected control (NI-P), and non-supplemented non-infected control (NN-P) groups. Ten days post-prebiotic supplementation (PPS), rabbits in groups PS-P and NI-P were infected orally with 5.0 × 104 sporulated oocysts of mixed Eimeria species. However, therapeutic experiment had prebiotic supplemented (PS-T) and untreated infected (UI-T) groups of naturally infected rabbits with Eimeria species. A significant reduction in oocyst count per gram feces (OPG) (p ≤ 0.05) was reported in the PS-P (57.33 × 103 ± 2.84) and NI-P (130.83 × 103 ± 43.38) groups during the experiment. Additionally, rabbits in groups (PS-P, 970.33 ± 31.79 g and NI-P, 870.66 ± 6.66 g) showed weight loss after infection. However, a significant (p ≤ 0.05) decrease in OPG was observed at day seven PPS in the PS-T group (4 × 103 ± 0.00) when compared with the UI-T group (32 × 103 ± 7.54). Furthermore, the PS-T group had a higher body weight than rabbits in the UI-T group. Histopathological findings of the intestinal tissues (duodenum, jejunum, and ileum) showed that the counts of the endogenous stages were significantly higher in the NI-P and UI-T groups than in the prebiotic-supplemented groups (PS-P and PS-T). Supplementation of the prebiotic did not have any adverse effects on biochemical parameters, such as AST, ALT, creatinine, total protein, and total cholesterol. In conclusion, prebiotic supplementation can be used to minimize the adverse effects of intestinal coccidiosis in rabbits, which in turn limits body weight loss, especially for the prophylaxis of coccidial infection.

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Molecular diagnosis of Eimeria stiedae in hepatic tissue of experimentally infected rabbits

Cited by 17 publications

References 22 publications

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae

Biochemical and Pathomorphological Investigations on Rabbits with Experimentally Induced Hepatic Eimeriosis

Prophylactic and Therapeutic Efficacy of Prebiotic Supplementation against Intestinal Coccidiosis in Rabbits

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