2005
DOI: 10.1007/s10096-005-0039-1
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Molecular diagnostics in tuberculosis

Abstract: Molecular diagnostics in tuberculosis has enabled rapid detection of Mycobacterium tuberculosis complex in clinical specimens, identification of mycobacterial species, detection of drug resistance, and typing for epidemiological investigation. In the laboratory diagnosis of tuberculosis, the nucleic acid amplification (NAA) test is rapid and specific but not as sensitive as culture of mycobacteria. The primary determinant of successful NAA testing for tuberculosis depends on the shedding of mycobacterial DNA i… Show more

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Cited by 119 publications
(113 citation statements)
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“…According to Cheng et al (30), early studies on nucleic acid amplification tests were carried out in laboratories that did not use clinical information in the decision-making process. However, clinical suspicion of tuberculosis is very important in determining the utility of these tests.…”
Section: Discussionmentioning
confidence: 99%
“…According to Cheng et al (30), early studies on nucleic acid amplification tests were carried out in laboratories that did not use clinical information in the decision-making process. However, clinical suspicion of tuberculosis is very important in determining the utility of these tests.…”
Section: Discussionmentioning
confidence: 99%
“…Detection of AFB in a smear requires more than 10,000 organisms/mL; nucleic acid amplification test could be very helpful in establishing the diagnosis of TB in smear-negative samples [47]. Nucleic acid amplification tests (NAAT) such as polymerase chain reaction (PCR) are rapid and specific but suffer from low sensitivity especially in AFB smear-negative cases.…”
Section: Tissue Diagnosismentioning
confidence: 99%
“…Several nucleic acid amplification and nucleic acid hybridization techniques have been introduced, but they were found to be less sensitive than culture-based techniques and are often complicated due to the presence of inhibitors (Nancy et al 2000, Cheng et al 2005. Alternatively, common techniques, such as the acid-fast bacillus (AFB) smear and löwenstein-Jensen (lJ) cultures, have limitations, such as low sensitivity, and require prolonged incubation (typically weeks).…”
mentioning
confidence: 99%