Molecular differences in genotroph forms of common wheat (Triticum aestivum L.) and their initial cultivars

Abstract

By the modified ISSR-amplification method the comparison of the structural organization of enzyme loci in the initial common wheat cultivars and their genotrophs induced with epimutagens: plant niacin acid and niacin acid and its derivatives produced from β-picoline fraction of coal-tar pitch (niacin acid nitrile, isocinchomeronic and benzoic acids) was carried out. It is shown that niacin acid influence causes in genotrophs specific band appearance in PCR-profiles obtained on the DNA of enzyme loci. All these before mentioned epimutagens induced in limits of each genotroph differently directed changes in number and intensity of bands of PCR-profiles

Changes in the organization of isozyme loci Me1 and Adh1 in-duced with triton X-100 in common wheat lines

“…By means of this method differences among plants carrying the same allele of isozyme locus were shown and also differences in the structure of marker isozyme locus evincing in different tissues of the same plant were revealed [19]. The differences of PCR-profiles of isozyme loci of common wheat genotrophs obtained by means of different epimutagens including nicotinic acid were shown [20]. In the aforementioned article, it was shown that natural epimutagens act more specifically causing the appearance of certain bands in PCR-profiles of investigated genes.…”