By the modified ISSR-amplification method the comparison of the structural organization of enzyme loci in the initial common wheat cultivars and their genotrophs induced with epimutagens: plant niacin acid and niacin acid and its derivatives produced from β-picoline fraction of coal-tar pitch (niacin acid nitrile, isocinchomeronic and benzoic acids) was carried out. It is shown that niacin acid influence causes in genotrophs specific band appearance in PCR-profiles obtained on the DNA of enzyme loci. All these before mentioned epimutagens induced in limits of each genotroph differently directed changes in number and intensity of bands of PCR-profiles
The study of PCR-profiles of isozyme loci Me1 and Adh1 in common wheat lines obtained by means of treatment of initial cultivar Alem with detergent Triton X-100 was carried out by the modified ISSRamplification method. It was demonstrated that exposure to Triton X-100 causes changes of PCR-profiles of enzyme loci. The obtained data are suggestive of the role of chromatin and nuclear membrane interaction in structural-functional genome organization.
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