Marker traits of variability induced in Triticum aestivum L. by nicotinic acid

Bogdanova, E. D.; Левитес, Е. В.; Махмудова, К. Х.

doi:10.1134/s1022795409030089

Cited by 5 publications

(6 citation statements)

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“…Chromosomes as well as other nuclear components such as RNA and DNA content variations are important somaclonal variation detection techniques that have widely been used (Bogdanova 2003;Nakano et al 2006;Fiuk et al 2010). Karyological analysis and observation of chromosomal aberration using light microscopy, oil immersion or other complex microscopy techniques have been successfully employed for detecting somaclonal variation in in vitro regenerants (Al-Zahim et al 1999;Raimondi et al 2001;Mujib et al 2007), however, it is time-consuming and often tedious especially where chromosomes are difficult to observe (Doležel 1997).…”

Section: Cytological Methodsmentioning

confidence: 98%

Somaclonal variation in plants: causes and detection methods

2010

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Plant tissue culture has become one of the fundamental tools of plant science research. It is extensively employed in the production, conservation and improvement of plant resources. The presence of somaclonal variation in populations derived from tissue culture is affecting the use of tissue culture negatively and has remained a major problem. Conversely, it is a source of new desirable clones/variants with better agronomic traits. In this review, we summarize the possible causes, detection methods and desirability of variants. Somaclonal variation is one of the most researched and reviewed topics. Hence, we restricted ourselves to outlining various examples which may be used as important references for researchers who intend to identify and/or characterize somaclonal variants while using tissue culture for research and production. Emphasis is placed on the negative effects of somaclonal variation. However, this review also includes examples of some useful variants generated as a result of somaclonal variation.

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Section: Cytological Methodsmentioning

confidence: 98%

Somaclonal variation in plants: causes and detection methods

2010

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“…Кіріспе. Ceлeкция тәжipибeciндe aлғaшқы мaтepиaл aлудың шeгiн кeңeйту үшiн бacтaпқы copттaн құнды бeлгiлepi бoйыншa epeкшeлeнeтiн мутaнтты линиялapды қoлдaнудың бoлaшaғы зop [1].…”

Section: жұмсақ бидай сорттарынан алынған мутантты линиялардың даму тunclassified

Genetic Analysis of the Types of Development of Mutant Lines From Common Wheat Varieties

Sarybay¹,

Chunetova²,

Iskakova³

et al. 2020

OF THE NATIONAL ACADEMY OF SCIENCES OF THE REPUBLIC OF KAZAKHS

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Among cereals, a special place is occupied by wheat as the main food of humanity and in improving the country's economy. The main task of breeding is to produce valuable wheat varieties that are stable to the unfavorable external environment of various natural climatic zones of Kazakhstan, consisting of a combination of valuable features. The prospects for the use of mutants in the process of hybridization are reflected in the achievements of world and domestic selection. The effect of various solutions of chemical compounds on the plant was observed from the first days during the growth of wheat sprouts. The growth of the first sprout of seeds treated with a certain solution of cadmium chloride and zinc chloride - 0.01%, the activity of cell division and structural disorders of chromosomes were manifested by variability in the varieties Kazakhstani3, Shagala, Zhenis and Lutescens 32. When treating dry wheat grain with a concentration of 0.1% cadmium chloride, it brought the growth of the Sprout to a lethal state, 0.01% inhibited the growth of the Sprout for 4 days, and in a 0.001% solution, the growth rate of the control grains was the same. On the contrary, a 0.1% vertical solution of zinc chloride inhibited the growth of the Sprout for 1 Week, 0.01% for three days. Among the studied concentrations, a 0.01% solution of cadmium chloride inhibited the growth of sprouts, causing chromosomal aberrations and morphological variability in cell division. Therefore, a concentration of 0.01% of cadmium salt was obtained as an optimal concentration to expand the limits of variability in wheat. In this regard, in our study, it was found that a 0.01% solution of cadmium chloride is an effective concentration that expands the limits of variability in wheat. The effect of this amount of solution leads to morphological changes in the plant (Binding of the stem, elongation of the earlobe, increase in the number and weight of grains in the headlobe, increase in the weight of 1000 grains, etc.), and changes in the characteristics of the M1 - M4 offspring are constantly inherited. Mutant lines L1, L2 and L3 differ in valuable breeding characteristics under the influence of a 0.01 percent solution of cadmium salt were obtained for the Kazakhstanskaya 3 and Shagala varieties. A genetic analysis of the type of development of these altered lines was carried out, as a result of which a long (16 cm) dense ear (0.80) changed from the Gull variety to a prismatic plant of the control variety (0.50), as a result of hybridization, its summer type (from the vrn gene) to the autumn type (Vrn gene). As a result of the study, Kazakhstanskaya 3, Kazakhstanskaya mutant 3 varieties contain the genes Vrn 1, Vrn 3. Well, it was found that Shagala varieties consist of the genes Vrnl, Vrn2. Varieties containing the genes Vrn1, Vrn3 show rapid maturation. However, they differed from each other in the period of intoxication.

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“…Equally with 5-azacytidin in the capacity of mater which is able to induce inheritable alterations to row of sexual generations researchers also started to use niacin acid (NA) [10][11][12]. Treatment of spring common wheat (Triticum aestivum L.) plants of the cultivar Kazakhstanskya-126 (K-126) with NA solutions resulted in appearance of the wheat form Genotroph-1 (G-1) having changed morphophysiological traits, enhanced viability and heightened resistance to extreme environmental conditions in comparison with the initial cultivar [10,11]. In crossing of these plants and the initial cultivar in hybrids F 1 the traits of changed plants were dominant.…”

Section: Introductionmentioning

confidence: 99%

“…High prices of NA of natural origin and low biological activity of synthetic NA produced from the synthetic β-picolines led to necessity of producing as NA as its analogues and derivatives from the β-picoline fraction of coal-tar pitch which are characterized with high biological activity. In the capacity of such analogues the following substances were synthesized and used in our investigations: niacin acid amid, niacin acid nitrile, isocinchomeronic acid, benzoic acid and others [10,11]. When using these analogues, appearance of tall, long-spiked genotrophs after the repeated (2 nd ) treatment of seed from generation in generation was also noted.…”

Section: Introductionmentioning

confidence: 99%

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Molecular differences in genotroph forms of common wheat (Triticum aestivum L.) and their initial cultivars

Vinichenko¹,

Bogdanova²,

Махмудова³

et al. 2010

ABB

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By the modified ISSR-amplification method the comparison of the structural organization of enzyme loci in the initial common wheat cultivars and their genotrophs induced with epimutagens: plant niacin acid and niacin acid and its derivatives produced from β-picoline fraction of coal-tar pitch (niacin acid nitrile, isocinchomeronic and benzoic acids) was carried out. It is shown that niacin acid influence causes in genotrophs specific band appearance in PCR-profiles obtained on the DNA of enzyme loci. All these before mentioned epimutagens induced in limits of each genotroph differently directed changes in number and intensity of bands of PCR-profiles

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Marker traits of variability induced in Triticum aestivum L. by nicotinic acid

Cited by 5 publications

References 5 publications

Somaclonal variation in plants: causes and detection methods

Somaclonal variation in plants: causes and detection methods

Genetic Analysis of the Types of Development of Mutant Lines From Common Wheat Varieties

Molecular differences in genotroph forms of common wheat (Triticum aestivum L.) and their initial cultivars

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