Molecular dissection of the tobacco Rb7 matrix attachment region (MAR): Effect of 5′ half on gene expression in rice

Verma, Deepika; Verma, Meenu; Dey, Moul; Jain, R. K.; Wü, Ray

doi:10.1016/j.plantsci.2005.05.021

Cited by 12 publications

(4 citation statements)

References 29 publications

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“…Matrix attachments regions are natural and abundant regions found in genomic DNA that are thought to attach to the matrix or scaffold of the nucleus. When positioned on the flanking ends of gene cassettes, some MARs have been shown to increase expression of transgenes and to reduce the incidence of gene silencing (Han et al, 1997;Abranches et al, 2005;Verma et al, 2005). It is hypothesized that MARs may act as a buffer to protect transgenes from neighboring chromosomal sequences that could destabilize their expression (Allen et al, 1993;Allen et al, 2000).…”

Section: Rb7 Marmentioning

confidence: 99%

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate

Braxton¹,

Richburg²,

York³

et al. 2017

Weed Technol

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Release of InformationDow AgroSciences LLC (DAS) is submitting the information in this petition for deregulation to USDA-APHIS as part of the regulatory process. By submitting this information, DAS does not authorize its release to any third party. If USDA receives a Freedom of Information Act request pursuant to 5 U.S.C., § 552 and 7 CFR Part 1 regarding this information, DAS expects that USDA will provide DAS with a copy of the material proposed to be released and the opportunity to object to the release of any information based on appropriate legal grounds (e.g., responsiveness, confidentiality, and/or competitive concerns). DAS understands that a copy of this information may be made available to the public in a reading room and upon individual request as part of a public comment period. Except in accordance with the foregoing, DAS does not authorize the release, publication or other distribution of this information (including website posting) without prior notice and consent from DAS.© 2013 Dow AgroSciences LLC. All Rights Reserved. This document is property of Dow AgroSciences LLC and is for use by the regulatory authority to which it has been submitted, and only in support of actions requested by Dow AgroSciences. All data and information herein must not be used, reproduced or disclosed to third parties for any other purpose without the written consent of Dow AgroSciences. USDA-APHIS Petition for Nonregulated Status of DAS-8191Ø-7 CottonPage 3 of 214 Dow AgroSciences LLC CertificationThe undersigned certifies that, to the best knowledge and belief of the undersigned, this petition includes all information and views on which to base a determination and that it includes all relevant data and information known to the petitioner, unfavorable as well as favorable, associated with DAS-8191Ø-7 cotton. SummaryDow AgroSciences LLC (herein referred to as "DAS") is submitting a Petition for Determination of Nonregulated Status for DAS-8191Ø-7 cotton. DAS requests from USDA Animal and Plant Health Inspection Service (APHIS) that cotton transformation event DAS-8191Ø-7 and any cotton lines derived from crosses between DAS-8191Ø-7 cotton and conventional cotton or biotechnology-derived cotton granted nonregulated status by APHIS, no longer be considered regulated articles under 7 CFR Part 340.DAS has developed transgenic cotton plants containing aad-12 and pat, which confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate. DAS-8191Ø-7 cotton will provide growers with greater flexibility in selection of herbicides for the improved control of economically important weeds; allow an increased application window for effective weed control; and provide an effective weed resistance management solution to the growing incidence of glyphosate resistant weeds.DAS-8191Ø-7 cotton plants have been genetically modified to express aryloxyalkanoate dioxygenase-12 (AAD-12). AAD-12 is an enzyme with an alpha ketoglutarate-dependent dioxygenase activity which results in metabolic inactivation of the herbi...

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Section: Rb7 Marmentioning

confidence: 99%

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate

Braxton¹,

Richburg²,

York³

et al. 2017

Weed Technol

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“…One of T-DNA region contains the chimeric gene pGt1-AmA1-nos, carrying the 915 bp desired AmA1 coding region (13), 1314 bp seed-specifi c Gt1 promoter (22) and nopaline synthase (nos) terminator. The chimeric gene is fl anked by 1.0 kb tobacco Rb 7 matrix attachment regions (MARs) for high-level transgene expression in rice (23), the other T-DNA region carrying a hygromycin resistance gene (hpt) driven by Caulifl ower mosaic virus (CaMV 35S) promoter. The pCDMAR-AmA1-hpt was transferred into Agrobacterium tumefaciens strain EHA105 by the freeze-thaw method (24), for use in rice transformation as follows.…”

Section: Plasmid Constructmentioning

confidence: 99%

Production of Marker-free Transgenic Rice (Oryza sativa L.) with Improved Nutritive Quality Expressing AmA1

Zhao

Zhang

et al. 2017

Iran. J. Biotechnol.

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Rice seed proteins are lacking essential amino acids (EAAs). Genetic engineering offers a fast and sustainable method to solve this problem as it allows the specific expression of heterologous EAA-rich proteins. The use of selectable marker gene is essential for generation of transgenic crops, but might also lead to potential environmental and food safety problems. Therefore, the production of marker-free transgenic crops is becoming an extremely attractive alternative and could contribute to the public acceptance of transgenic crops. The present study was conducted to examine whether can be expressed specifically in rice seeds, and generate marker-free transgenic rice with improved nutritive value. was transferred into rice using-mediated co-transformation system with a twin T-DNA binary vector and its integration in rice genome was confirmed by southern blot. Transcription of was analyzed by Real-Time PCR and its expression was verified by western analysis. Protein and amino acid content were measured by the Kjeldahl method and the high-speed amino acid analyzer, respectively. Five selectable marker-free homozygous transgenic lines were obtained from the progeny. The expression of recombinant was confirmed by the observation of a 35 kDa band in SDS-PAGE and western blot. Compared to the wild-type control, the total protein contents in the seeds of five homozygous lines were increased by 1.06~12.87%. In addition, the content of several EAAs, including lysine, threonine, and valine was increased significantly in the best expressing line. The results indicated that the amino acid composition of rice grain could be improved by seed-specific expression of .

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“…For example, the 3 0 MAR associated with the tobacco Rb7 gene was found to significantly augment the expression of a flanked transgene when compared to controls lacking this MAR (Allen et al 1996;Cheng et al 2001;Ü lker et al 1999), and in some instances was also able to lessen variability between transgenic lines through a reduction in transgene silencing (Halweg et al 2005;Mankin et al 2003;Verma et al 2005). Similarly, the presence of chicken lysozyme A MAR elements (Phi- Van and Strätling 1988) flanking transgenes in tobacco and rice has been found to reduce variability by reducing silencing effects (Mlynárová et al 1994;Oh et al 2005).…”

Section: Barrier Insulators In Transgenic Plantsmentioning

confidence: 99%

Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators

2011

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The genetic transformation of plants has become a necessary tool for fundamental plant biology research, as well as the generation of engineered plants exhibiting improved agronomic and industrial traits. However, this technology is significantly hindered by the fact that transgene expression is often highly variable amongst independent transgenic lines. Two of the major contributing factors to this type of inconsistency are inappropriate enhancer-promoter interactions and chromosomal position effects, which frequently result in mis-expression or silencing of the transgene, respectively. Since the precise, often tissue-specific, expression of the transgene(s) of interest is often a necessity for the successful generation of transgenic plants, these undesirable side effects have the potential to pose a major challenge for the genetic engineering of these organisms. In this review, we discuss strategies for improving foreign gene expression in plants via the inclusion of enhancer-blocking insulators, which function to impede enhancer-promoter communication, and barrier insulators, which block the spread of heterochromatin, in transgenic constructs. While a complete understanding of these elements remains elusive, recent studies regarding their use in genetically engineered plants indicate that they hold great promise for the improvement of transgene expression, and thus the future of plant biotechnology.

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Molecular dissection of the tobacco Rb7 matrix attachment region (MAR): Effect of 5′ half on gene expression in rice

Cited by 12 publications

References 29 publications

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate

Production of Marker-free Transgenic Rice (Oryza sativa L.) with Improved Nutritive Quality Expressing AmA1

Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators

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Molecular dissection of the tobacco Rb7 matrix attachment region (MAR): Effect of 5′ half on gene expression in rice

Cited by 12 publications

References 29 publications

Resistance of Enlist™(AAD-12) Cotton to Glufosinate

Resistance of Enlist™(AAD-12) Cotton to Glufosinate

Production of Marker-free Transgenic Rice (Oryza sativa L.) with Improved Nutritive Quality Expressing AmA1

Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators

Contact Info

Product

Resources

About

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate

Resistance of Enlist^™(AAD-12) Cotton to Glufosinate