Molecular diversity of bacteria in Yunnan yellow cattle (Bos taurs) from Nujiang region, China

Abstract

The rumen content of four Yunnan Yellow Cattle (Bos taurs) were collected to determine the bacteria diversity by using 16S rRNA gene sequence analysis. A total of 129 sequences were examined and the sequences were referred as 107 OTU (Operational Taxonomy Unit) according to the similarity level of 97% in gene sequence. Similarity analysis revealed that Yunnan Yellow Cattle had 12 sequences (10 OTU) shared 97% or greater similarity with cultured rumen bacteria Butyrivibrio fibrisolvens, Succiniclasticum ruminis, Ruminococcus bromii, Clostridium proteoclasticum, Ruminococcus flavefaciens, Pseudobutyrivibrio ruminis, Jeotgalicoccus psychrophilus, and Prevotella ruminicola, which accounting for 9.3% of the total clones (9.2% of the total OTU). The further 12 sequences (9 OTU) shared 90-97% similarity with cultured bacteria Clostridium aminobutyricum, butyrate-producing bacterium, Schwartzia succinivorans, Prevotella ruminicola, Eubacterium ruminantium, Ruminococcus albus, and Clostridium termitidis, also accounting for 9.3% of the total sequences (8.3% of the total OTU). The remaining 105 sequences (90 OTU) shared less than 90% similarity with cultured bacteria, accounting for 81.4% of the total sequences (82.5% of the total OTU). According to the phylogenetic analysis, all sequences were phylogenetically placed within phyla of low G+C subdivision (accounting for 72.1 and 72.5% of the total clones and OTU, respectively) and CFB subdivision (Cytophaga-Flexibacter-Bacteroides; accounting for 27.9 and 27.5% of the total clones and OTU, respectively). Among the examined clones, rare bacteria Jeotgalicoccus psychrophilus was detected in the rumen of cattle.

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

“…The prime function of Succiniclasticum ruminis is to ferment succinate and quantitatively convert it to propionate. Succiniclasticum has been shown to exist in many ruminants, including yaks [17,23], cow [73], cattle [39,53], buffaloes [85] and goat [47]. In the present study, it was also found in Tibetan sheep.…”

The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus

“…and Lactococcus sp. Sequences belonging to Bacteroidetes, typical ruminant foregut bacteria [ 60 – 63 ], were identified in adult worms, but not L3 or eggs ( Fig 1 and Table 1 ). Rumen bacteria can remain alive in abomasal contents, particularly when the pH is raised by the presence of abomasal parasites [ 64 ] and either dead or live rumen bacterial cells could contribute to the DNA and the sequences subsequently identified in the adult worm samples.…”

Biliary Microbiota, Gallstone Disease and Infection with Opisthorchis felineus

“…felineus lead to the modification of composition of the bile microbiome. Specifically, most of the phylotypes that differed were detected in higher abundance in bile during opisthorchiasis although some phylotypes decreased; Jeotgalicoccus psychrophilus , a Gram-positive halophile [ 51 , 52 ] was included among the latter. Lactobacillus spp.…”