Molecular diversity of<i>Helicoverpa armigera</i>Hubner (Noctuidae: Lepidoptera) in India

Asokan, R.; Nagesha, S. N.; Manamohan, M.; Krishnakumar, N.; Mahadevaswamy, H. M.; Rebijith, K. B.; Prakash, M.N.; Chandra, G. Sharath

doi:10.1080/00305316.2012.699264

Cited by 15 publications

(11 citation statements)

References 22 publications

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“…The H. armigera and H. zea population genetics were not structured according to space, time (winter and summer crops), or host (crops). Unstructured genetic networks have been reported for other populations of these two pest species in other parts of the world, which were based on several molecular markers, including mtDNA, allozymes, and microsatellites [7] , [24] , [25] , [28] , [29] , [30] . Both species showed wide spatial haplotype distributions, and no genetic relationships were identified using a haplotype network analysis or an AMOVA.…”

Section: Discussionmentioning

confidence: 99%

Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

et al. 2014

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Helicoverpa armigera is one of the primary agricultural pests in the Old World, whereas H. zea is predominant in the New World. However, H. armigera was first documented in Brazil in 2013. Therefore, the geographical distribution, range of hosts, invasion source, and dispersal routes for H. armigera are poorly understood or unknown in Brazil. In this study, we used a phylogeographic analysis of natural H. armigera and H. zea populations to (1) assess the occurrence of both species on different hosts; (2) infer the demographic parameters and genetic structure; (3) determine the potential invasion and dispersal routes for H. armigera within the Brazilian territory; and (4) infer the geographical origin of H. armigera. We analyzed partial sequence data from the cytochrome c oxidase subunit I (COI) gene. We determined that H. armigera individuals were most prevalent on dicotyledonous hosts and that H. zea were most prevalent on maize crops, based on the samples collected between May 2012 and April 2013. The populations of both species showed signs of demographic expansion, and no genetic structure. The high genetic diversity and wide distribution of H. armigera in mid-2012 are consistent with an invasion period prior to the first reports of this species in the literature and/or multiple invasion events within the Brazilian territory. It was not possible to infer the invasion and dispersal routes of H. armigera with this dataset. However, joint analyses using sequences from the Old World indicated the presence of Chinese, Indian, and European lineages within the Brazilian populations of H. armigera. These results suggest that sustainable management plans for the control of H. armigera will be challenging considering the high genetic diversity, polyphagous feeding habits, and great potential mobility of this pest on numerous hosts, which favor the adaptation of this insect to diverse environments and control strategies.

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Section: Discussionmentioning

confidence: 99%

Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

et al. 2014

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“…[ 28 ] and the binding modes were generated by PyMOL V0.99 (Schrödinger, New York, NY, USA.) [ 29 ]. The polar hydrogen atoms were added to the crystal structures of the AMPK via the biopolymer module and the Gasteiger-Huckel charges were loaded on the atoms of proteins.…”

Section: Methodsmentioning

confidence: 99%

Identification of Direct Activator of Adenosine Monophosphate-Activated Protein Kinase (AMPK) by Structure-Based Virtual Screening and Molecular Docking Approach

Huang

Sun

Zhou

et al. 2017

IJMS

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Adenosine monophosphate-activated protein kinase (AMPK) plays a critical role in the regulation of energy metabolism and has been targeted for drug development of therapeutic intervention in Type II diabetes and related diseases. Recently, there has been renewed interest in the development of direct β1-selective AMPK activators to treat patients with diabetic nephropathy. To investigate the details of AMPK domain structure, sequence alignment and structural comparison were used to identify the key amino acids involved in the interaction with activators and the structure difference between β1 and β2 subunits. Additionally, a series of potential β1-selective AMPK activators were identified by virtual screening using molecular docking. The retrieved hits were filtered on the basis of Lipinski's rule of five and drug-likeness. Finally, 12 novel compounds with diverse scaffolds were obtained as potential starting points for the design of direct β1-selective AMPK activators.

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“…For the synthesis of dsRNA template, specific primers having T7 promoter sequence at 5′ ends were synthesized (Table S1). The previously cloned recombinant plasmids harboring individual HaTry1 and HaTry13 genes of H. armigera [24] were used for in vitro dsRNA synthesis. Additionally, to silence both the genes, pyramided dsRNA of the both genes was used.…”

Section: Synthesis Of Double-stranded Rnamentioning

confidence: 99%

Double-Stranded RNA-Mediated Suppression of Trypsin-Like Serine Protease (t-SP) Triggers Over-Expression of Another t-SP Isoform in Helicoverpa armigera

Chandra

Asokan

Manamohan

et al. 2017

Appl Biochem Biotechnol

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High diversity of digestive proteases is considered to be the key factor in the evolution of polyphagy in Helicoverpa armigera. Serine proteases (SPs) contribute ~85% of the dietary protein digestion in H. armigera. We investigated the dynamics of SP regulation in the polyphagous pest, H. armigera using RNA interference (RNAi). HaTry1, an isoform of SP, expressed irrespective of the composition of the diet, and its expression levels were directly proportional to the larval growth rate. Therefore, HaTry1 was silenced by delivering 10 and 20 μg concentrations of double-stranded RNA through semi-synthetic diet. This led to a drastic reduction in the target gene transcript levels that manifested in a significant reduction in the larval weight initially, but the larvae recovered in later stages despite continuous dsRNA treatment. This was probably due to the compensatory effect by over-expression of HaTry13 (31-folds), another isoform of SP. Phylogenetic analysis of H. armigera SPs revealed that the over-expressed isoform was closely related to the target gene as compared to the other tested isoforms. Further, silencing of both the isoforms (HaTry1 and HaTry13) caused the highest reduction in the larval weight and there was no larval growth recovery. These findings provide a new evidence of the existence of compensatory effect to overcome the effect of silencing individual gene with RNAi. Hence, the study emphasizes the need for simultaneous silencing of multiple isoforms.

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Molecular diversity ofHelicoverpa armigeraHubner (Noctuidae: Lepidoptera) in India

Cited by 15 publications

References 22 publications

Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

Identification of Direct Activator of Adenosine Monophosphate-Activated Protein Kinase (AMPK) by Structure-Based Virtual Screening and Molecular Docking Approach

Double-Stranded RNA-Mediated Suppression of Trypsin-Like Serine Protease (t-SP) Triggers Over-Expression of Another t-SP Isoform in Helicoverpa armigera

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