Molecular diversity of the entomopathogenic fungal Metarhizium community within an agroecosystem

Steinwender, Bernhardt M.; Enkerli, J.; Widmer, Franco; Eilenberg, Jørgen; Thorup‐Kristensen, Kristian; Meyling, Nicolai V.

doi:10.1016/j.jip.2014.09.002

Cited by 68 publications

(66 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plates were repeatedly observed through a low magnifying stereomicroscope (Olympus SZX9, 40X magnification) and, if any emergence of nematodes were observed, they were discarded no matter if a fungal growth was present or absent. Any possibility of cross-contamination or external contamination was carefully monitored as described by Steinwender et al (2014). No colony forming units (CFUs) were observed in any of the tests for contaminations.…”

Section: Fungal Isolation and Screeningmentioning

confidence: 99%

“…Since then it has been used quite often in numerous studies as the only method for EPF isolations, in the past three decades (Chandler et al 1997, Bidochka et al 1998, Ali-Shtayeh et al 2003, Meyling and Eilenberg 2006, Quesada-Moraga et al 2007, Sevim et al 2009, Fisher et al 2011, Muñiz-Reyes et al 2014, Pérez-González et al 2014, Fernández-Salas et al 2017, Gan and Wickings 2017, Kirubakaran et al 2018. Similarly, in few other studies, insect baiting using T. molitor is the only method used for the EPF recovery (Sánchez-Peña et al 2011, Steinwender et al 2014). …”

Section: Insects Baiting Of Soils For Epf Recoverymentioning

confidence: 99%

See 1 more Smart Citation

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Sharma

Oliveira

Torres

et al. 2018

View full text Add to dashboard Cite

Entomopathogenic fungi (EPF) are the natural enemies of insect-pests. However, EPF recoveries can be influenced by the soil habitat-type(s) incorporated and/or the bait-insect(s) used. Galleria mellonella (GM) as bait-insect, i.e. 'Galleria-bait', is arguably the most common methodology, which is sometimes used solely, to isolate EPF from soils. Insect baiting using Tenebrio molitor (TM) has also been employed occasionally. Here 183 soils were used to estimate the functional diversity of EPF in Portuguese Douro vineyards (cultivated habitat) and adjacent hedgerows (semi-natural habitat), using the TM bait method. Moreover, to study the effect of insect baiting on EPF recovery, 81 of these 183 soil samples were also tested for EPF occurrences using the GM bait method. Twelve species were found in 44.26% ± 3.67% of the total of 183 soils. Clonostachys rosea f. rosea was found in maximum soils (30.05% ± 3.38%), followed by Beauveria bassiana (12.57% ± 2.37%), Purpureocillium lilacinum (9.29% ± 2.14%) and Metarhizium robertsii (6.01% ± 1.75%). Beauveria pseudobassiana (P < 0.001), C. rosea f. rosea (P = 0.006) and RESEARCH ARTICLELav Sharma et al. / MycoKeys 38: 1-23 (2018) 2 Cordyceps cicadae (P=0.023) were isolated significantly more from hedgerows, highlighting their sensitivities towards agricultural disturbances. Beauveria bassiana (P = 0.038) and M. robertsii (P = 0.003) were isolated significantly more using GM and TM, respectively. Principal component analysis revealed that M. robertsii was associated both with TM baiting and cultivated habitats, however, B. bassiana was slightly linked with GM baiting only. Ecological profiles of B. bassiana and P. lilacinum were quite similar while M. robertsii and C. rosea f. rosea were relatively distant and distinct. To us, this is the first report on (a) C. cicadae isolation from Mediterranean soils, (b) Purpureocillium lavendulum as an EPF worldwide; and (c) significant recoveries of M. robertsii using TM over GM. Overall, a 'Galleria-Tenebrio-bait method' is advocated to study the functional diversity of EPF in agroecosystems.

show abstract

Section: Fungal Isolation and Screeningmentioning

confidence: 99%

Section: Insects Baiting Of Soils For Epf Recoverymentioning

confidence: 99%

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Sharma

Oliveira

Torres

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Similar abundance and diversity of Metarhizium spp. has been reported for soils of different habitats and geographical regions around the world (Steinwender et al 2014;Enkerli et al 2016;Kepler et al 2015;Castro et al 2016). Metarhizium strains were re-isolated from about one third of the wireworm cadavers recovered from FCBK treatments.…”

Section: Discussionmentioning

confidence: 92%

Preventive application of an entomopathogenic fungus in cover crops for wireworm control

et al. 2017

Self Cite

View full text Add to dashboard Cite

Efficacy of the Metarhizium brunneum Petch (Hypocreales: Clavicipitaceae) strain ART2825 for control of wireworms (Agriotes obscurus (L.), Coleoptera: Elateridae) was examined in a semi-field pot experiment. Pots were treated in late summer during sowing of spring oat as a cover crop. Survival of wireworms was assessed four weeks after their release in October 2013, and 30 weeks after release in April 2014. Viability and persistence of the fungus was determined by counting colony forming units from substrate samples and microsatellite analyses of recovered Metarhizium isolates. The number of colonies detected in the substrate in October 2013 increased with increasing concentrations of applied conidia, and no significant reduction was observed at the second evaluation date in April 2014. Increasing conidia application rates significantly increased mycosis and reduced wireworm survival, to a level comparable to that of treatment using insecticide-coated oat seeds. The preventive application of M. brunneum conidia to reduce wireworm populations in cover crops, preceding a damage-sensitive crop like potatoes, may be a promising biocontrol strategy.

show abstract

“…LEGER et al, 1992;TIGANO-MILANI;GOMES;SOBRAL, 1995;YANAKA-SCHAFER et al, 2008); (3) isolados originados de uma mesma espécie de hospedeiros ou de grupos de espécies semelhantes (LUAN et al, 2013) e (4) isolados de um mesmo tipo de ambiente agrícola (FEGAN et al, 1993;STEINWENDER et al, 2011STEINWENDER et al, , 2014 (LEAL et al, 1994(LEAL et al, , 1997PIPE et al, 1995;ST. LEGER et al, 1992 Oxford, v. 18, n. 9, p. 865-901, 2008.…”

Section: Importância Do Gênerounclassified

“…haplotypes in the soil may be useful for maximizing its use as biological control agent. MILNER; TRUEMAN, 2000;ENKERLI et al, 2005ENKERLI et al, , 2009FEGAN et al, 1993;LEAL et al, 1994;LUAN et al, 2013;PIPE et al, 1995;STEINWENDER et al, 2011STEINWENDER et al, , 2014VELÁSQUEZ et al, 2007). No Brasil, tais estudos se restringem a isolados que se encontram depositados em coleções de entomopatógenos ou que foram coletados em um determinado tipo de habitat/hospedeiro associado a uma região ou estado, como é o caso do CentroOeste brasileiro (LOPES et al, 2013a(LOPES et al, , 2013bLOPES et al, 2014;REZENDE et al, 2015;ROCHA et al, 2013;SOSA-GÓMEZ et al, 2009;TIGANO-MILANI;GOMEZ;SOBRAL, 1995;YANAKA-SCHAFER et al, 2008).…”

Section: Introductionunclassified

Abundância de fungos entomopatogênicos da ordem Hypocreales e diversidade genética de Metarhizium spp. isolados de amostras de solo de áreas representativas de cinco biomas brasileiros

Zanardo¹

View full text Add to dashboard Cite

Molecular diversity of the entomopathogenic fungal Metarhizium community within an agroecosystem

Cited by 68 publications

References 27 publications

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

Preventive application of an entomopathogenic fungus in cover crops for wireworm control

Abundância de fungos entomopatogênicos da ordem Hypocreales e diversidade genética de Metarhizium spp. isolados de amostras de solo de áreas representativas de cinco biomas brasileiros

Contact Info

Product

Resources

About