Molecular diversity study within holy basil species (<i>Ocimum tenuiflorum</i>L.) using ISSR and RAPD markers

Malav, Pavan Kumar; Singh, Rakesh; Pandey, Anjula; Bhatt, KC

doi:10.5958/0974-0112.2015.00097.3

Cited by 2 publications

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“…ISSR markers also have the ability to amplify DNA fragments that are present at an amplifiable distance between two identical microsatellite repeat regions orientated in opposite direction (Reddy et al 2002). High polymorphism of ISSR was also reported in many previous studies, as in the case of Mentha arvensis, Ocimum (Malav et al 2015;Dhawan et al, 2016;Chaturvedi et al 2018;Alves et al 2019;Gupta et al 2021). Previous studies have also recommended that SCoT markers work better performance than other molecular marker systems in other plants (Luo et al, 2011).…”

Section: Speciessupporting

confidence: 72%

Detection of genetic variation among five basil species by using ISSR, SCOT and SRAP markers

Ahmed

Hussein

El-Maaty

et al. 2022

ijhs

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Background: Eight inter-simple sequence repeats (ISSR), 14 Start codon-targeted (SCoT), and eleven sequence-related amplified polymorphisms were used to determine genetic diversity among five basil species (Ocimum tenuiflorum, Ocimum kilimandscharicum Guerk, Ocimum basilicum L., Ocimum americanum, and Ocimum basilicum var. minimum). Results: All of the markers' PCR products can be used to discriminate between the different basil species. The ISSR has the highest polymorphism percentage (73.1%), followed by SCoT (57.2%) and SRAP (55.6%). According to the combined data from the three markers, the basil species used were clustered into four groups, each with extremely excellent coordination between the similarity matrix and dendrograms. Species-specific markers have been identified. The SCoT analysis generates the highest number of species-specific marker (17) compared with ISSR and SRAP analysis which generated 14 and 13 markers respectively. Conclusions: These markers can be considered as a useful marker to improve Ocimum characteristics.

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Section: Speciessupporting

confidence: 72%

Detection of genetic variation among five basil species by using ISSR, SCOT and SRAP markers

Ahmed

Hussein

El-Maaty

et al. 2022

ijhs

View full text Add to dashboard Cite

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Diversity of Sweet Basil Accessions from Croatian National Plant Gene Bank Based on Amplified Fragment Length Polymorphism Markers

Varga,

Vidak,

Liber

et al. 2024

Agronomy

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This study investigates genetic diversity among five morphotypes and five chemotypes of Ocimum basilicum L. (sweet basil) using amplified fragment length polymorphism (AFLP) markers. Conducted on 80 basil accessions from the Collection of Medicinal and Aromatic Plants of the National Plant Gene Bank of the Republic of Croatia, this research aims to enhance the conservation and utilization of sweet basil’s genetic resources. AFLP analysis using extracted genomic DNA revealed high levels of polymorphism, particularly within the True basil morphotype, which displayed 95.6% polymorphic markers. The results showed genetic differentiation between the morphotypes, particularly between the ’green’ and ’purple’ groups, and within certain chemotypes, such as the High-linalool chemotype (Chemotype A). Principal coordinate analysis (PCoA) and Bayesian clustering further highlighted the genetic structures, with some admixture observed, particularly in the Purple basil B morphotype. Analysis of molecular variance (AMOVA) indicated that most of the genetic diversity was between accessions, emphasizing the value of individual variability. These findings underscore the genetic potential within sweet basil accessions, offering valuable insights for future breeding programs aimed at selecting basil cultivars with tailored biochemical and morphological traits suited for pharmaceutical, culinary, and ornamental applications. The study provides an important basis for the conservation and improvement of basil genetic resources.

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Molecular diversity study within holy basil species (Ocimum tenuiflorumL.) using ISSR and RAPD markers

Cited by 2 publications

References 0 publications

Detection of genetic variation among five basil species by using ISSR, SCOT and SRAP markers

Detection of genetic variation among five basil species by using ISSR, SCOT and SRAP markers

Diversity of Sweet Basil Accessions from Croatian National Plant Gene Bank Based on Amplified Fragment Length Polymorphism Markers

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