Molecular docking analysis of Allium chinense compounds as Secreted Aspartyl Proteinase-5 (SAP5) inhibitor

Hartanto, A; Naibaho, Frans Grovy; Panjaitan, D; Lutfia, A; Munir, E

doi:10.1088/1755-1315/977/1/012017

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…There are several virulence factors of C. albicans, including phenotypic switching, morphological dimorphism, thigmotropism, adhesion proteins, secretion of hydrolase enzymes and biofilm formation [10]. The 3 main hydrolase enzymes secreted by C. albicans include Secreted Aspartyl Protease (SAP), phospholipase and hemolysin [11,12]. Secreted Aspartyl Protease (SAP) produced by C. albicans is classified into 10 types according to the coding gene.…”

Section: Introductionmentioning

confidence: 99%

Efficacy Comparison of Oral and Intravaginal Lactobacillus plantarum Administration on Secreted Aspartyl Protease-5 (SAP5) Levels in Vulvovaginal Candidiasis

Fristiyanti,

Matin,

Ratnaningrum

et al. 2023

Trends Sci

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Vulvovaginal candidiasis (VVC) is the most common fungal infection in the vaginal mucosa caused by an overgrowth of Candida albicans. The pathogenesis of C. albicans is determined by the presence of virulence secretory factors. Secreted Aspartyl Protease 5 (SAP5) is the dominant virulence factor that plays a critical role in the morphology switching of yeast cells into hyphae which causes tissue invasion and induces an immunopathological response. Levels of SAP5 are often associated with the severity various types of infection. As a potential supportive therapy to treat VVC, identification of the probiotic’s administration route determines the level of efficacy of probiotics. This study aims to compare the decrease in SAP5 levels between oral and intravaginal administration of L. plantarum. The study was true experimental using a completely randomized pretest-posttest group design. There were 32 female rats were divided into 8 groups i.e negative control, positive control, 3 groups of oral and 3 of intravaginal administration. Positive control and 6 treatment group were injected with estradiol valerate 0.5 mg and then inoculated with suspension of C. albicans 2×107 CFU to create VVC model. L. plantarum was administered orally and intravaginally with 3 concentration variants i.e. 2.25×1010, 4.5×1010 and 9×1010 CFU. Both of oral and intravaginal group were given with probiotics for 14 days. SAP5 levels were measured by ELISA from vaginal lavage sample. The statistical analysis used was the Paired T-Test to measure SAP5 levels pre-post oral and intravaginal administration of L. plantarum. The results showed a significant decrease in SAP5 levels in oral administration (p = 0.001 < 0.05) and intravaginal administration (p = 0.000 < 0.05). From the results of the statistical analysis, it can be concluded that both oral and intravaginal administration of the probiotic L. plantarum has the same efficacy in reducing the virulence factor of C. albicans in cases of VVC. HIGHLIGHTS Lactobacillus plantarum is potential supportive therapy for Vulvovaginal Candidiasis Efficacy plantarum identified through oral and intravaginal administration SAP5 is an important virulence factor in pathogenesis of Vulvovaginal Candidiasis Efficacy’s rate plantarum was analyzed based on SAP5 levels in vaginal fluid GRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Efficacy Comparison of Oral and Intravaginal Lactobacillus plantarum Administration on Secreted Aspartyl Protease-5 (SAP5) Levels in Vulvovaginal Candidiasis

Fristiyanti,

Matin,

Ratnaningrum

et al. 2023

Trends Sci

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show abstract

“…There are several virulence factors for C. albicans, including phenotypic transitions, morphological dimorphism, thigmotropism, adhesion proteins, secretion of hydrolase enzymes, and biofilm formation [12]. The three main hydrolase enzymes secreted by C. albicans include Secreted Aspartyl Protease (SAP), phospholipase, and hemolysin [13,14]. Secreted Aspartyl Protease (SAP) produced by C. albicans is the main invasive protease enzyme and has an important role in the pathogenesis of C. albicans [15].…”

Section: Introductionmentioning

confidence: 99%

Correlation between Secreted Aspartyl Protease-5 (SAP5) on Increasing Vaginal Acidity in Vulvovaginal Candidiasis

Nur Sophia Matin,

Regina Ayu Fristiyanti,

Safrina Dewi Ratnaningrum

et al. 2023

a-jhr

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Introduction: Vulvovaginal candidiasis (VVC) is an infection of the vaginal mucosa that extends to the vulva due to excessive growth of Candida albicans, causing a progressive decrease of Lactobacillus spp., as the main of normal flora. Decrease in the number of Lactobacillus spp. causes a reduction of lactic acid production that it was causing increasing vaginal pH. The increase in pH is due to the progressive growth of C. albicans colonies in the vaginal mucosa. In addition, the mechanism of candidiasis is also influenced by the secretion of virulence factors by C. albicans. Secreted Aspartyl Protease-5 (SAP5) is the dominant virulence factor that plays a role in changing the morphology of yeast cells into hyphae. This study aims to identify the correlation between SAP5 levels and vaginal pH. Material and Methods: This research was conducted in vivo using a sample of Rattus norvegicus with a posttest randomized experimental group design approach. This study has two groups, i.e., Negative Control (NC) and Positive Control group (PC). SAP5 and pH samples were taken from vaginal fluids and then measured using ELISA and a digital pH meter. The statistical analysis used was Pearson Correlation to analyze the correlation between SAP5 and pH levels. Results: There was a strong correlation (R=0.846) that means the increase of SAP5 is related to the increase of vaginal acidity in the PC group. Conclusion: The increase in SAP5 levels is directly followed by an increase in the pH value of vaginal fluids,

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Molecular docking analysis of Allium chinense compounds as Secreted Aspartyl Proteinase-5 (SAP5) inhibitor

Cited by 2 publications

References 18 publications

Efficacy Comparison of Oral and Intravaginal Lactobacillus plantarum Administration on Secreted Aspartyl Protease-5 (SAP5) Levels in Vulvovaginal Candidiasis

Efficacy Comparison of Oral and Intravaginal Lactobacillus plantarum Administration on Secreted Aspartyl Protease-5 (SAP5) Levels in Vulvovaginal Candidiasis

Correlation between Secreted Aspartyl Protease-5 (SAP5) on Increasing Vaginal Acidity in Vulvovaginal Candidiasis

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