This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

Penggunaan pestisida sintetik yang tidak terkontrol dapat menyebabkan pencemaran lingkungan karena meningggalkan senyawa pestisida di tanah dan perairan. Bioremediasi adalah salah satu cara untuk mengurangi cemaran senyawa kimia berbahaya di lingkungan dengan menggunakan mikroorganisme. Tujuan penelitian ini ialah untuk memperoleh isolat bakteri potensial  penghasil biosurfaktan dari laut Belawan Sumatera Utara dan untuk mengetahui aktivitas biosurfaktannya. Isolasi bakteri dilakukan dengan menggunakan media selektif Bushnell-Hass Agar (BHA) yang mengandung 12 ppm pestisida berbahan aktif karbosulfan sebagai sumber karbon. Aktivitas biosurfaktan dilakukan dengan metode Drop collapsing test dan konsentrasi biosurfaktan dilakukan dengan metose orsinol yang dimodifikasi. Hasil penelitian menunjukkan 9 isolat berhasil diisolasi dan mampu tumbuh pada media yang mengandung karbosulfan. Aktivitas biosurfaktan tertinggi dihasilkan oleh isolat FB6 yang ditandai dengan pembentukan emulsi sebesar 5,627 mL dan produksi biosurfaktan tertinggi ditunjukkan oleh isolat FB7 dengan konsentrasi 54,6 ppm.

Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method.  Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.

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