Molecular Epidemiology and Antibiotic Resistance Patterns of &lt;i&gt;Salmonella enterica&lt;/i&gt; from Southwestern Greece

Spiliopoulou, Iris; Zografou, Sofia; Goula, Amalia; Dimitracopoulos, G.; Christofidou, Myrto

doi:10.1159/000109768

Cited by 10 publications

(9 citation statements)

References 21 publications

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“…In our hospital alone, there were about 150e200 reported cases each year. The seasonal variation of salmonellae in Hong Kong was similar to that reported in Europe, 15 with the number of cases of salmonellosis increasing from AprileMay and decreasing after AugusteSeptember as hot and humid weather provided an optimum environment for the salmonellae to grow on food and thus more cases during this time of the year. S. Enteritidis is the most common serovar isolated followed by S. Typhimurium, both of which were responsible for more than half of all salmonellosis cases each year.…”

Section: Discussionsupporting

confidence: 75%

“…15 In contrast, it might show that PFGE analysis might not be powerful enough to discriminate between strains. Recently, other typing methods, namely multiple-locus variable-number tandemrepeat analysis (MLVA) and multilocus sequence typing (MLST), have been used to type salmonellae.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that boys tend to be more adventurous and so more likely to be exposed to more contaminated foods. 15 The lowest salmonellosis rate in age group >80 might be due to the lower prevalence of risky behaviours among the elderly. 21 Relatively more women got infected than men at age >10.…”

Section: Discussionmentioning

confidence: 99%

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Increasing quinolone resistance and multidrug resistant isolates among Salmonella enterica in Hong Kong

Lo¹,

Chu²,

Ling³

2012

Journal of Infection

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Increasing quinolone resistance and multidrug resistant isolates among Salmonella enterica in Hong Kong

Lo¹,

Chu²,

Ling³

2012

Journal of Infection

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“…The Enteritidis serovar was not assessed by PFGE because, according to the literature, these isolates have low clonal diversity (Spiliopoulou et al, 2007). …”

Section: Discussionmentioning

confidence: 99%

Characteristics of Quinolone Resistance in Salmonella spp. Isolates from the Food Chain in Brazil

et al. 2017

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Salmonella spp. is an important zoonotic pathogen related to foodborne diseases. Despite that quinolones/fluoroquinolones are considered a relevant therapeutic strategy against resistant isolates, the increase in antimicrobial resistance is an additional difficulty in controlling bacterial infections caused by Salmonella spp. Thus, the acquisition of resistance to quinolones in Salmonella spp. is worrisome to the scientific community along with the possibility of transmission of resistance through plasmids. This study investigated the prevalence of plasmid-mediated quinolone resistance (PMQR) in Salmonella spp. and its association with fluoroquinolone susceptibility in Brazil. We evaluated 129 isolates, 39 originated from food of animal sources, and 14 from environmental samples and including 9 from animals and 67 from humans, which were referred to the National Reference Laboratory of Enteric Diseases (NRLEB/IOC/RJ) between 2009 and 2013. These samples showed a profile of resistance for the tested quinolones/fluoroquinolones. A total of 33 serotypes were identified; S. Typhimurium (63) was the most prevalent followed by S. Enteritidis (25). The disk diffusion test showed 48.8% resistance to enrofloxacin, 42.6% to ciprofloxacin, 39.53% to ofloxacin, and 30.2% to levofloxacin. According to the broth microdilution test, the resistance percentages were: 96.1% to nalidixic acid, 64.3% to enrofloxacin, 56.6% to ciprofloxacin, 34.1% to ofloxacin, and 30.2% to levofloxacin. Qnr genes were found in 15 isolates (8 qnrS, 6 qnrB, and 1 qnrD), and the aac(6′)-Ib gene in 23. The integron gene was detected in 67 isolates with the variable region between ±600 and 1000 bp. The increased detection of PMQR in Salmonella spp. is a serious problem in Public Health and must constantly be monitored. Pulsed-field gel electrophoresis was performed to evaluated clonal profile among the most prevalent serovars resistant to different classes of quinolones. A total of 33 pulsotypes of S. Typhimurium were identified with a low percentage of genetic similarity (≤65%). This result demonstrates the presence of high diversity in the resistant clones evaluated in this study.

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“…In this study, the prevalence of Salmonella Enteritidis was 54%, which showed the dominance of this serovar in Iran. Recent reports from Brazil, Poland, Malaysia, China and Greece have shown the same serovar as the dominant serovar with the frequencies ranging from 34% to 86%, which reveals a coincident rise of this serovar around the world [15] , [16] , [17] , [18] , [19] .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Salmonella enterica serotype Enteritidis isolates from food and human samples by serotyping, antimicrobial resistance, plasmid profiling, (GTG)5-PCR and ERIC-PCR

Fardsanei

Nikkhahi

Bakhshi

et al. 2016

New Microbes and New Infections

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In recent years, Salmonella enterica serovar Enteritidis has been a primary cause of human salmonellosis in many countries. The major objective of this study was to investigate genetic diversity among Salmonella Enteritidis strains from different origins (food and human) by Enterobacterial Repetitive Intergenic Consensus (ERIC) -PCR, as well as to assess their plasmid profiling and antimicrobial resistance. A total of 30 Salmonella Enteritidis isolates, 15 from food samples (chicken, lamb, beef and duck meats) and 15 from clinical samples were collected in Tehran. Identification of isolates as Salmonella was confirmed by using conventional standard biochemical and serological tests. Multiplex-PCR was used for serotyping of isolates to identify Salmonella Enteritidis. Antimicrobial susceptibility testing to 16 agents founds drug resistance patterns among Salmonella Enteritidis isolates. No resistance was observed to cephalexin, ceftriaxone, ceftazidime and cefotaxime, ciprofloxacin, imipenem or meropenem, chloramphenicol and gentamicin. The highest resistance (96.7%) was observed to nitrofurantoin. Seven plasmid profiles (P1–P7) were detected, and a 68-kb plasmid was found in all isolates. Two different primers; ERIC and (GTG)5 were used for genotyping, which each produced four profiles. The majority of clinical and food isolates fell into two separate common types (CTs) with a similar percentage of 95% by ERIC-PCR. Using primer (GTG)5, 29 isolates incorporated in three CTs with 70% of isolates showing a single banding pattern. Limited genetic diversity among human and food isolates of Salmonella Enteritidis may indicate that contaminated foods were possibly the source of human salmonellosis. These results confirmed that ERIC-PCR genotyping has limited discriminatory power for Salmonella Enteritidis of different origin.

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Molecular Epidemiology and Antibiotic Resistance Patterns of <i>Salmonella enterica</i> from Southwestern Greece

Cited by 10 publications

References 21 publications

Increasing quinolone resistance and multidrug resistant isolates among Salmonella enterica in Hong Kong

Increasing quinolone resistance and multidrug resistant isolates among Salmonella enterica in Hong Kong

Characteristics of Quinolone Resistance in Salmonella spp. Isolates from the Food Chain in Brazil

Molecular characterization of Salmonella enterica serotype Enteritidis isolates from food and human samples by serotyping, antimicrobial resistance, plasmid profiling, (GTG)5-PCR and ERIC-PCR

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