Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum β-lactamase-producing Klebsiella pneumoniae isolated in Portugal

Mendonça, Nuno; Ferreira, Eugénia; Louro, Deolinda; Caniça, Manuela

doi:10.1016/j.ijantimicag.2008.11.014

Cited by 46 publications

(38 citation statements)

References 31 publications

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“…In this set of strains, 69.4% (134/193) were multidrug-resistant (MDR), as presenting a reduced susceptibility to 3 or more structurally unrelated antibiotics, among which were fluoroquinolones (97%), aminoglycosides (91.8%), and trimethoprim-sulfamethoxazole (72.4%). Clinical isolates were tested for the presence of bla ESBL and bla PMAβ genes, by using polymerase chain reaction and sequencing, as described previously (Mendonça et al, 2009;Pérez-Pérez and Hanson, 2002). Overall, the presence of ESBLs was confirmed in 167 of 193 isolates and the bla CTX-M genes in 154 of 167 isolates (CTX-M-ESBL group), such as bla CTX-M-1 (6, 3.9%), bla CTX-M-3 (13, 8.4%), bla CTX-M-9 (2, 1.3%), bla CTX-M-14 (4, 2.6%), bla CTX-M-15 (123, 79.9%), and bla CTX-M-32 (6, 3.9%).…”

Section: R (%) Ir (%) R (%) Ir (%) R (%) Ir (%) R (%) Ir (%) R (%) Irmentioning

confidence: 99%

Emergence and risk factors of β-lactamase–mediated resistance to oxyimino-β-lactams in Enterobacteriaceae isolates

Manageiro

Ferreira

Jones-Dias

et al. 2012

Diagnostic Microbiology and Infectious Disease

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We studied 193 Enterobacteriaceae isolates presenting diminished susceptibility to oxyimino-cephalosporins recovered in a Portuguese hospital (2004)(2005)(2006)(2007)(2008). CTX-M-3 producers, firstly detected in Portugal, were associated with a Klebsiella pneumoniae microepidemic clone. Production of CTX-M-type enzymes (CTX-M-1/-3/-9/-14/-15/-32), age ≥65 years, and nosocomial infection were risk factors for higher nonsusceptibility to oxyimino-β-lactams. CMY-2 and DHA-1 β-lactamases were only identified in 1% of isolates.

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Section: R (%) Ir (%) R (%) Ir (%) R (%) Ir (%) R (%) Ir (%) R (%) Irmentioning

confidence: 99%

Emergence and risk factors of β-lactamase–mediated resistance to oxyimino-β-lactams in Enterobacteriaceae isolates

Manageiro

Ferreira

Jones-Dias

et al. 2012

Diagnostic Microbiology and Infectious Disease

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“…Currently, the ESBL CTX-M are the most frequently isolated in western and eastern Algeria (Ahmed et al, 2012;Nedjai et al, 2012), Tunisia (Elhani et al, 2011), Portugal (Mendonça et al, 2009), China (Liu et al, 2009), in Taiwan (Shu et al, 2010 and many countries worldwide. In our study, the phenotype of the DHA-1-producing strains was characterized in three cefoxitin-resistant strains, two strains (Kp18, Kp55.2) were isolated in the women medicine service, and one strain (kp73) was isolated in the intensive care unit (ICU).…”

Section: Discussionmentioning

confidence: 99%

First characterization of CTX-M-15 and DHA-1 -lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

Lagha¹,

Abdelouahid²,

Hassaïne³

et al. 2014

Afr. J. Microbiol. Res.

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Extended-spectrum β-lactamases (ESBL) are a problem of great concern owing to the potential transmission of resistance to others bacterial species. Our study aims to investigate the ESBL and plasmid-mediated β-lactamases produced by Klebsiella pneumoniae strains isolated from 2010 to 2012, among patients hospitalized in "Ahemida Ben Adjila" hospital, Laghouat, Algeria, and to seek a possible clonal dissemination. Antimicrobial susceptibility testing was performed by the agar diffusion method, the characterization of resistance genes to β-lactam antibiotics was performed by PCR amplification and gene sequencing, and molecular typing was performed by ERIC-PCR. In total, of 112 clinical strains of K. pneumoniae isolated, nine isolates produced an ESBL. Antibiotics susceptibility testing showed a complete resistance to the majority of third-generation cephalosporins and a very frequent resistance to aminoglycosides and fluoroquinolones resistance. PCR analysis and sequencing showed that all isolates produced ESBL CTX-M-15; three strains of them also produced the cephalosporinase DHA-1. Molecular typing showed that most strains were not related; only three strains that produced CTX-M-15 and DHA-1 had identical profiles suggesting a clonality link. This study revealed the dissemination of ESBL CTX-M-15 and plasmid-mediated AmpC cephalosporinase DHA-1 in K. pneumoniae. This is a first report of these enzymes at the Laghouat hospital.

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“…K. pneumoniae INSRA6884 was isolated in 2006 from the sputum of a 92-year-old woman in an internal medicine service at the Centro Hospitalar in Coimbra, Portugal (28). The Escherichia coli DH5␣ ⌬ampC strain and E. coli BL21(DE3) were used for initial cloning experiments and overexpression of the ␤-lactamase-encoding gene, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, many recent reports have described the emergence of class A ESBLs belonging to other families, such as GES, VEB, PER, and the worldwidedisseminated CTX-M family (2,21). The GES family, specifically, GES-1, has also been reported in many areas including Portugal (28).…”

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Characterization of the Inhibitor-Resistant SHV β-Lactamase SHV-107 in a Clinical Klebsiella pneumoniae Strain Coproducing GES-7 Enzyme

Manageiro

Ferreira

Cougnoux

et al. 2012

Antimicrob Agents Chemother

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The clinical Klebsiella pneumoniae INSRA6884 strain exhibited nonsusceptibility to all penicillins tested (MICs of 64 to >2,048 g/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2,048 to 512 g/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genes bla GES-7 and a new ␤-lactamase gene, bla SHV-107 , which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producing Escherichia coli strain exhibited only a ␤-lactam resistance phenotype with respect to amoxicillin, ticarcillin, and amoxicillinclavulanate combination. The kinetic parameters of the purified SHV-107 enzyme revealed a high affinity for penicillins. However, catalytic efficiency for these antibiotics was lower for SHV-107 than for SHV-1. No hydrolysis was detected against oxyimino-␤-lactams. The 50% inhibitory concentration (IC 50 ) for clavulanic acid was 9-fold higher for SHV-107 than for SHV-1, but the inhibitory effects of tazobactam were unchanged. Molecular dynamics simulation suggested that the Thr235Ala substitution affects the accommodation of clavulanate in the binding site and therefore its inhibitory activity.

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Molecular epidemiology and antimicrobial susceptibility of extended- and broad-spectrum β-lactamase-producing Klebsiella pneumoniae isolated in Portugal

Cited by 46 publications

References 31 publications

Emergence and risk factors of β-lactamase–mediated resistance to oxyimino-β-lactams in Enterobacteriaceae isolates

Emergence and risk factors of β-lactamase–mediated resistance to oxyimino-β-lactams in Enterobacteriaceae isolates

First characterization of CTX-M-15 and DHA-1 -lactamases among clinical isolates of Klebsiella pneumoniae in Laghouat Hospital, Algeria

Characterization of the Inhibitor-Resistant SHV β-Lactamase SHV-107 in a Clinical Klebsiella pneumoniae Strain Coproducing GES-7 Enzyme

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