Molecular epidemiology and evolution of drug-resistant genes in the malaria parasite Plasmodium falciparum in southwestern Nigeria

Oboh, Mary Aigbiremo; Singh, Upasana Shyamsunder; Antony, Hiasindh Ashmi; Ndiaye, Daouda; Badiane, Aïda Sadikh; Ali, Nazia; Bharti, Praveen K.; Das, Ashim

doi:10.1016/j.meegid.2018.10.007

Cited by 13 publications

(16 citation statements)

References 54 publications

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“…The frequency of the Pfdhfr triple-mutant haplotype (IRNI) was significantly higher (95.7%, 155/162) than was that of double-mutant haplotypes (ICNI, 2.5%; NRNI, 0.6%) and wild-type haplotypes (NCSI, 1.8%) (p<0.05). This haplotype profiling was consistent with neighbouring countries, including Gabon, 37 Cameroon 34 and Nigeria 38 (Figure 1). IRNI and NRNI are the two most common Pfdhfr haplotypes worldwide.…”

Section: Frequency Of Pfdhfr and Pfdhps Haplotypessupporting

confidence: 82%

“…A significant difference in haplotype profiles was found between East African countries and West African countries along the Atlantic Ocean (Figure 2). SGKAA has been reported to be the dominant haplotype in most African countries along the Atlantic Ocean, including Cameroon, 33 Nigeria, 38 Gabon, 37 Angola, 35 Democratic Republic of Congo 41 and Bioko Island, and SGEAA is the major haplotype in East African countries, such as Uganda, 29 Tanzania, 29 Kenya 36 and Malawi. 32 The wild-type haplotype (SAKAA) is rare in almost all African and Asian countries but prevalent in Haiti 42 and Papua New Guinea.…”

Section: Frequency Of Pfdhfr and Pfdhps Haplotypesmentioning

confidence: 99%

“…To assess the relationship among Pfdhfr sequences between Bioko and global P.falciparum isolates, 603 sequences, including 352 from this study and our previous study 25 and 251 of other African and Asian countries (India, Congo, and Nigeria) downloaded from NCBI, 34,38,41 were used to construct a haplotype network (Figure 3). For all SNPs (nonsynonymous and synonymous mutations) located in the Pfdhfr gene, 21 distinct haplotypes (H_1 to H_21) were found worldwide ( Figure 3).…”

Section: Relationship Among Pfdhfr and Pfdhps Sequences Between Biokomentioning

confidence: 99%

“…These sequences included 327 from this study and our previous study 25 and 262 of other African and Asian countries (India, Congo, and Nigeria) downloaded from NCBI. 34,38,41 For all SNPs (nonsynonymous and synonymous mutations) in the Pfdhps gene, a total of 23 distinct haplotypes (H_1 to H_23) were identified (Figure 4). Eight haplotypes are shared by Pfdhps sequences from at least two countries; 15 haplotypes were found for one sequence.…”

Section: Dovepressmentioning

confidence: 99%

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Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011–2017

Lin¹,

Li²,

Huang

et al. 2020

IDR

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Antimalarial drug resistance is one of the major challenges in global efforts to control and eliminate malaria. In 2006, sulfadoxine-pyrimethamine (SP) replaced with artemisinin-based combination therapy (ACT) on Bioko Island, Equatorial Guinea, in response to increasing SP resistance, which is associated with mutations in the dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes. Patients and Methods: To evaluate the trend of molecular markers associated with SP resistance on Bioko Island from 2011 to 2017, 179 samples collected during active case detection were analysed by PCR and DNA sequencing. Results: Pfdhfr and Pfdhps gene sequences were obtained for 90.5% (162/179) and 77.1% (138/179) of the samples, respectively. For Pfdhfr, 97.5% (158/162), 95.7% (155/162) and 98.1% (159/162) of the samples contained N51I, C59R and S108N mutant alleles, respectively. And Pfdhps S436A, A437G, K540E, A581G, and A613S mutations were observed in 25.4% (35/138), 88.4% (122/138), 5.1% (7/138), 1.4% (2/138), and 7.2% (10/138) of the samples, respectively. Two classes of previously described Pfdhfr-Pfdhps haplotypes associated with SP resistance and their frequencies were identified: partial (IRNI-SGKAA, 59.4%) and full (IRNI-SGEAA, 5.5%) resistance. Although no significant difference was observed in different time periods (p>0.05), our study confirmed a slowly increasing trend of the frequencies of these SP-resistance markers in Bioko parasites over the 7 years investigated. Conclusion: The findings reveal the general existence of SP-resistance markers on Bioko Island even after the replacement of SP as a first-line treatment for uncomplicated malaria. Continuous molecular monitoring and additional control efforts in the region are urgently needed.

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Section: Frequency Of Pfdhfr and Pfdhps Haplotypessupporting

confidence: 82%

Section: Frequency Of Pfdhfr and Pfdhps Haplotypesmentioning

confidence: 99%

Section: Relationship Among Pfdhfr and Pfdhps Sequences Between Biokomentioning

confidence: 99%

Section: Dovepressmentioning

confidence: 99%

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Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among Plasmodium falciparum Isolates on Bioko Island, Equatorial Guinea: 2011–2017

Lin¹,

Li²,

Huang

et al. 2020

IDR

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“…E mergence and spread of antimalarial resistance in Plasmodium falciparum parasites have been major obstacles to global malaria control and eradication in past decades (1). Because chloroquine (CQ) resistance has spread throughout Africa since the early 1990s, the antifolate combination of sulfadoxine-pyrimethamine (SP) was widely introduced for the treatment of uncomplicated P. falciparum malaria (2). Unfortunately, an increase of parasite resistance to SP occurred, leading to a situation similar to that involving CQ resistance.…”

mentioning

confidence: 99%

Mutation Profile of pfdhfr and pfdhps in Plasmodium falciparum among Returned Chinese Migrant Workers from Africa

Sun

Wei

et al. 2019

Antimicrob Agents Chemother

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We evaluated markers of sulfadoxine-pyrimethamine (SP) resistance in Plasmodium falciparum among 254 returned migrant workers in China from Africa from 2013 to 2016. High prevalences of pfdhfr (97.2%) and pfdhps (96.5%) mutations were observed. The partially resistant genotype was homogeneously distributed in Africa with a modestly high prevalence (48%), whereas the super resistant genotype was only found in West Africa with a very low frequency (1.2%). The findings provided baseline data about the molecular markers of SP resistance.

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Application of machine and deep learning algorithms in optical microscopic detection of Plasmodium: A malaria diagnostic tool for the future

Ikerionwu

Ugwuishiwu²,

Okpala³

et al. 2022

Photodiagnosis and Photodynamic Therapy

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Molecular epidemiology and evolution of drug-resistant genes in the malaria parasite Plasmodium falciparum in southwestern Nigeria

Cited by 13 publications

References 54 publications

<p>Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among <em>Plasmodium falciparum</em> Isolates on Bioko Island, Equatorial Guinea: 2011–2017</p>

<p>Trends in Molecular Markers Associated with Resistance to Sulfadoxine-Pyrimethamine (SP) Among <em>Plasmodium falciparum</em> Isolates on Bioko Island, Equatorial Guinea: 2011–2017</p>

Mutation Profile of pfdhfr and pfdhps in Plasmodium falciparum among Returned Chinese Migrant Workers from Africa

Application of machine and deep learning algorithms in optical microscopic detection of Plasmodium: A malaria diagnostic tool for the future

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