Molecular Epidemiology and Virulence Characteristics of Klebsiella pneumoniae Strains Isolated from Hospital-Associated Infections

Abstract

Abstract:Purpose: The present study aimed to confirm by classical and molecular laboratory methods hospitalassociated outbreaks due to virulent Klebsiella pneumoniae strains.Methods: Eighty three Klebsiella pneumoniae strains isolated from new-born patients, adults and hospital environment and devices in five hospital units, were analyzed for resistance to antibiotics, including last generation cephalosporins, sensitivity to bacteriophages and pulsed-field gel electrophoresis profiles in order to evaluate the epidemiological relatedness and their clonal spreading. Polymerase chain reaction targeting fur genes and several subtractive sequences (SL 002, SL 003, SL 019, SL 020, SL 021 and SL 025) was used for virulence assessment.Results: More than 50% of strains were resistant to third generation cephalosporins and among them 69% were extended spectrum beta-lactamase producers. Phage typing associated with pulse field gel electrophoresis documented the clonal dispersion of strains. Studying the distribution of virulence sequence, our results reveal that fur gene is present in all strains and among the subtractive sequences the most frequent is SL 020 followed by SL 019. None of the analyzed sequences are present in all clinical isolates and none of the bacterial strains carry all these sequences pointing out the heterogeneity of Klebsiella pneumoniae population. Conclusions:Phage typing method associated with pulse field gel electrophoresis typing and genetic profile for virulence indicated the occurrence of hospital associated-infections produced by Klebsiella pneumoniae strains. Moreover, the results reveal that virulence pattern could be used as a molecular marker in order to define strains which are involved in the process of the development of infectious diseases.

ESBL Detection and Comparison of Antibiotics Resistance Pattern of Klebsiella Pneumoniae Isolated from Healthy and Acute Respiratory Tract Infected Camels

“…The results from this investigation is similar to that of (Damian et al, 2009;Lim et al, 2009) who reported 33.34% and 23.5% ESBL producing strains from human clinical cases respectively. Similarly (Khanfar et al, 2009) reported 17% ESBL positive K. pneumoniae strains from nosocomial and community-acquired infections and all ESBL positive isolates were sensitive to imipenem while in contrast to present study resistant to gentamicin and ciprofloxacin.…”

“…(Parasakthi et al, 2000) studied nosocomial outbreak of multidrug-resistant Klebsiella pneumoniae and reported that ESBL producing isolates were completely resistant to ceftazidime and sensitive to imipenem and ciprofloxacin, which is similar to the present study. In the present study, we were able to identify different resistotypes similar to that of (Damian et al, 2009) that of (Mansour et al, 2014) who studied the antibiotic resistance pattern of K. pneumoniae obtained from nasopharyngeal swab of respiratory tract infected camels and other domestic animals. They reported that K. pneumoniae isolates were resistant to β-lactams antibiotics like cefotaxime, ceftazidime, ceftriaxone and aztreonam with 100% resistance to amoxicillin and ampicillin.…”

Neonatal sepsis caused by Klebsiella

“…Формирование «госпитальных штаммов» клебсиелл в основном ассоциируется с их способностью продуцировать β-лактамазы расширенного спектра и карбапенемазы [11]. Инфекции, вызванные этими штаммами бактерий, отличаются резистентностью возбудителя к большинству широко используемых в практике цефалоспоринов и, как следствие, значимой летальностью [13,14]. К причинам неэффективности антибиотикотерапии относят также способность клебсиелл к формированию биопленки на поверхности различных медицинских изделий, особенно на сосудистых и мочевых катетерах, эндотрахеальных трубках [15,16].…”

Pathogenicity of Klebsiella pneumonia isolate from meat, meat products and Human in experimentally infected in rabbits

“…Other infections caused by this organism include urinary tract infections, wound infections, abscesses, sepsis, infections of the blood stream, inflammation and diarrhea (Hackstein et al, 2013). K. pneumonia strains exhibit different virulence factors which give the bacteria the ability to invade the host, including the structures of capsular polysaccharides (CPS; the K antigen), lipopolysaccharide (LPS; the O antigen), secreted toxins, drug resistance and genetics , serum resistance, production of urea and enterotoxin, type 1 and type 3 adhesions, factors involved in aggregative adhesions and siderophores (Damian et al, 2009;Abbott, 2003;Eisenstein and Zaleznik, 2000;Chang et al, 2000;Podschun and Ullman, 1998;Cheng et al, 1991). Capsule plays a very important role in virulence (Sikarwar and Batra, 2011).…”