Molecular epidemiology of Acinetobacter baumannii in different hospitals in Tripoli, Lebanon using bla
              OXA-51-like sequence based typing

Rafei, Rayane; Pailhoriès, Hélène; Hamzé, Monzer; Eveillard, Matthieu; Mallat, Hassan; Dabboussi, Fouad; Joly-Guillou, Marie-Laure; Kempf, Marie

doi:10.1186/s12866-015-0441-5

Cited by 66 publications

(57 citation statements)

References 49 publications

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“…In accordance with other studies performed in Lebanon, IC II was by far the most prevalent clone among the A. baumannii isolates (Rafei et al, 2014) and OXA-23-like is the most disseminated (Rafei et al, 2015). These findings are also similar to those reported from other Mediterranean countries (Di Popolo et al, 2011).…”

Section: Discussionsupporting

confidence: 91%

“…In 2012, 88% of 724 A. baumannii isolates recovered from various Lebanese hospitals were found to be resistant to imipenem (Hammoudi et al, 2015a). Although OXA-24-like (Hammoudi et al, 2015b) and OXA-58-like (Zarrilli et al, 2008) have been detected in this country, OXA-23-like seems to be the most prevalent among CRAB isolates (Rafei et al, 2015). …”

Section: Introductionmentioning

confidence: 97%

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Acinetobacter baumannii Isolated from Lebanese Patients: Phenotypes and Genotypes of Resistance, Clonality, and Determinants of Pathogenicity

Hajjar

Suárez

Daoud

2016

Front. Cell. Infect. Microbiol.

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Introduction: Acinetobacter baumannii is a nosocomial pathogen that usually affects critically ill patients. High mortality rates have been associated with MDR A. baumannii infections. Carbapenem resistance among these isolates is increasing worldwide and is associated with certain International Clones (ICs) and oxacillinases (OXAs). Moreover, this organism possesses a wide range of virulence factors, whose expression is not yet fully understood. In this study, clinical A. baumannii isolates are characterized in terms of antibiotic resistance, mechanisms of carbapenem resistance, clonality, and virulence.Materials and Methods: A. baumannii clinical isolates (n = 90) where obtained from a tertiary care center in Beirut, Lebanon. API 20NE strips in addition to the amplification of blaOXA−51−like were used for identification. Antibiotic susceptibility testing by disk diffusion was then performed in addition to PCRs for the detection of the most commonly disseminated carbapenemases. Clonality was determined by tri-locus PCR typing and doubling times were determined for isolates with varying susceptibility profiles. Biofilm formation, hemolysis, siderophore production, proteolytic activity, and surface motility was then determined for all the isolates. Statistical analysis was then performed for the determination of associations.Results and Discussion: 81 (90%) of the isolates were resistant to carbapenems. These high rates are similar to other multi-center studies in the country suggesting the need of intervention on a national level. 74 (91.3%) of the carbapenem resistant isolates harbored blaOXA−23−like including two that also harbored blaOXA−24−like. 88.9% of the A. baumannii isolates pertained to ICII and three other international clones were detected, showing the wide dissemination of clones into geographically distinct locations. Virulence profiles were highly diverse and no specific pattern was observed. Nevertheless, an association between motility, siderophore production, and biofilm formation was detected (p < 0.05).Conclusions: A very high rate of carbapenem resistance was detected, showing the need for immediate intervention. IC II and OXA-23-like were the most disseminated, reflecting their international dissemination. No specific associations were made between virulence and resistance, but instead associations among certain virulence factors were found. Investigating a more clonally diverse pool of isolates could help in the determination of associations between virulence and resistance.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Acinetobacter baumannii Isolated from Lebanese Patients: Phenotypes and Genotypes of Resistance, Clonality, and Determinants of Pathogenicity

Hajjar

Suárez

Daoud

2016

Front. Cell. Infect. Microbiol.

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“…2226C was isolated in Turkey in 2009 while ABC002 was collected in Egypt in 2011 (https ://pubml st.org/). ST158 isolates were also reported in Lebanon between 2011 and 2013 [42]. The recognition of Ab-Pak-cluster-7 in Pakistan has then expanded the geographic distribution of ST158 beyond the Middle East [43][44][45][46].…”

Section: Ab-pak-cluster-7 a New Member Of Clone St158mentioning

confidence: 99%

“…ST1 and ST1106 shared identical alleles in 5/7 of the loci. Ab-Pak-cluster-7 was assigned to ST158 (41,42,13,1,5,4,14). The individual isolates Ab-Pak-Pesh-04 and Ab-Pak-Pesh-22 belonged to ST25 (3, 3, 2, 4, 7, 2, 4) and ST23 (1,3,10,1,4,4,4), respectively.…”

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confidence: 99%

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

Karah

Khalid

Wai

et al. 2020

Ann Clin Microbiol Antimicrob

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Background: Acinetobacter baumannii is a Gram-negative opportunistic pathogen with a notorious reputation of being resistant to antimicrobial agents. The capability of A. baumannii to persist and disseminate between healthcare settings has raised a major concern worldwide. Methods: Our study investigated the antibiotic resistance features and molecular epidemiology of 52 clinical isolates of A. baumannii collected in Pakistan between 2013 and 2015. Antimicrobial susceptibility patterns were determined by the agar disc diffusion method. Comparative sequence analyses of the ampC and bla OXA-51-like alleles were used to assign the isolates into clusters. The whole genomes of 25 representative isolates were sequenced using the MiSeq Desktop Sequencer. Free online applications were used to determine the phylogeny of genomic sequences, retrieve the multilocus sequence types (ST), and detect acquired antimicrobial resistance genes.Results: Overall, the isolates were grouped into 7 clusters and 3 sporadic isolates. The largest cluster, Ab-Pak-cluster-1 (bla OXA-66 and ISAba1-ampC-19) included 24 isolates, belonged to ST2 and International clone (IC) II, and was distributed between two geographical far-off cities, Lahore and Peshawar. Ab-Pak-clusters-2 (bla OXA-66 , ISAba1-ampC-2), and -3 (bla OXA-66 , ISAba1-ampC-20) and the individual isolate Ab-Pak-Lah-01 (ISAba1-bla OXA-66 , ISAba1-ampC-2) were also assigned to ST2 and IC II. On the other hand, Ab-Pak-clusters-4 (bla OXA-69 , ampC-1), -5 (bla OXA-69 , ISAba1-ampC-78), and -6A (bla OXA-371 , ISAba1-ampC-3) belonged to ST1, while Ab-Pak-cluster-6B (bla OXA-371 , ISAba1-ampC-8) belonged to ST1106, with both ST1 and ST1106 being members of IC I. Five isolates belonged to Ab-Pak-cluster-7 (bla . This cluster corresponded to ST158, showed a well-delineated position on the genomic phylogenetic tree, and was equipped with several antimicrobial resistance genes including bla OXA-23 and bla . Conclusions:Our study detected the occurrence of 7 clusters of A. baumannii in Pakistan. Altogether, 6/7 of the clusters and 45/52 (86.5%) of the isolates belonged to IC I (n = 9) or II (n = 36), making Pakistan no exception to the global domination of these two clones. The onset of ST158 in Pakistan marked a geographical dispersal of this clone beyond the Middle East and brought up the need for a detailed characterization.

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“…In Lebanon, numerous recent studies reported an increasing high rates of resistant bacteria isolates from clinical and commensal sources to most antimicrobials [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. However, few data about the bacterial ear pathogens in Lebanon is available.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic susceptibility of ear pathogens isolated from patients in Tripoli, north of Lebanon

Hamzé

Osman²,

Mallat³

et al. 2017

int. arab. j antimicrob. agents

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Background: Ear infection is one of the most widespread illness usually caused by bacteria or viruses, and which can be extremely painful. They are the most common reason for parents to bring their children to clinicians. The aim of this study is to determine the prevalence and antibiotic susceptibility pattern of common bacterial ear pathogens isolated from patients in Tripoli, North Lebanon during the period of 7 years. Methods:A total of 481 bacterial ear pathogens were isolated from deep ear swab specimens of patients who came to Nini hospital clinical laboratory in Tripoli, North Lebanon. Culture, identification and antibiotic susceptibility of suspected isolates were carried out through conventional tests. API identification and serotyping were performed according to standard protocols proposed by the Référentiel en Microbiologie Médicale and the recommendations of the European Committee on Antimicrobial Susceptibility Testing.Results: Out of 481, non-Enterobacteriaceae Gram negative rods were predominant (36.4%) followed by Staphylococcus aureus (24.8%), Enterobacteriace ae (16.8%), Haemophilus influenzae (10%), β-hemolytic streptococci (6.2%), Streptococcus pneumoniae (5.4%) and Moraxella catarrhalis (0.4%). Susceptibility testing showed the prevalence rate of methicillin-resistant S. aureus was 18.5% and of penicillin-non-susceptible pneumococci was 30.8%. Enterobacteriaceae and H. influenzae had susceptibilities of 18.5% and 80% to ampicillin, respectively. P. aeruginosa susceptibility to piperacillin-tazobactam and imipenem were very low, with 9.4% and 6.6%, respectively. Overall, H. influenzae and S. pyogenes isolates were also among the more susceptible isolates to antibiotics.

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Molecular epidemiology of Acinetobacter baumannii in different hospitals in Tripoli, Lebanon using bla OXA-51-like sequence based typing

Cited by 66 publications

References 49 publications

Acinetobacter baumannii Isolated from Lebanese Patients: Phenotypes and Genotypes of Resistance, Clonality, and Determinants of Pathogenicity

Acinetobacter baumannii Isolated from Lebanese Patients: Phenotypes and Genotypes of Resistance, Clonality, and Determinants of Pathogenicity

Molecular epidemiology and antimicrobial resistance features of Acinetobacter baumannii clinical isolates from Pakistan

Prevalence and antibiotic susceptibility of ear pathogens isolated from patients in Tripoli, north of Lebanon

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